RT21_HUMAN
ID RT21_HUMAN Reviewed; 87 AA.
AC P82921; A0A075B746; Q5TB11; Q9BST6;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 3.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=28S ribosomal protein S21, mitochondrial;
DE Short=MRP-S21;
DE Short=S21mt;
DE AltName: Full=Mitochondrial small ribosomal subunit protein bS21m {ECO:0000303|PubMed:25838379};
GN Name=MRPS21; Synonyms=RPMS21; ORFNames=MDS016;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ARG-50.
RX PubMed=11402041; DOI=10.1074/jbc.m103236200;
RA Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A.,
RA Watanabe K.;
RT "Proteomic analysis of the mammalian mitochondrial ribosome. Identification
RT of protein components in the 28S small subunit.";
RL J. Biol. Chem. 276:33181-33195(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-50.
RC TISSUE=Hematopoietic stem cell;
RA Huang C., Qian B., Tu Y., Gu W., Wang Y., Han Z., Chen Z.;
RT "Novel genes expressed in hematopoietic stem/progenitor cells from
RT myelodysplastic syndrome patients.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-50.
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-50.
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-50.
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-50.
RC TISSUE=Lung, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION.
RX PubMed=11279123; DOI=10.1074/jbc.m100727200;
RA Koc E.C., Burkhart W., Blackburn K., Moseley A., Spremulli L.L.;
RT "The small subunit of the mammalian mitochondrial ribosome: identification
RT of the full complement of ribosomal proteins present.";
RL J. Biol. Chem. 276:19363-19374(2001).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [10]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=25838379; DOI=10.1126/science.aaa1193;
RA Amunts A., Brown A., Toots J., Scheres S.H., Ramakrishnan V.;
RT "Ribosome. The structure of the human mitochondrial ribosome.";
RL Science 348:95-98(2015).
CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-
CC SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small
CC (28S) and a large (39S) subunit. The 28S small subunit contains a 12S
CC ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large
CC subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial
CC valine transfer RNA (mt-tRNA(Val)), which plays an integral structural
CC role, and 52 different proteins. {ECO:0000269|PubMed:25838379}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:25838379}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bS21 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG14953.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAG14953.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB049955; BAB41008.1; -; mRNA.
DR EMBL; AF182417; AAG14953.1; ALT_INIT; mRNA.
DR EMBL; AK289467; BAF82156.1; -; mRNA.
DR EMBL; AC242988; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL138795; CAI22820.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW53558.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW53559.1; -; Genomic_DNA.
DR EMBL; BC004566; AAH04566.2; -; mRNA.
DR EMBL; BC006554; AAH06554.1; -; mRNA.
DR CCDS; CCDS950.1; -.
DR RefSeq; NP_061870.1; NM_018997.3.
DR RefSeq; NP_114107.1; NM_031901.5.
DR PDB; 3J9M; EM; 3.50 A; AQ=1-87.
DR PDB; 6NU2; EM; 3.90 A; AQ=2-87.
DR PDB; 6NU3; EM; 4.40 A; AQ=2-87.
DR PDB; 6RW4; EM; 2.97 A; Q=2-87.
DR PDB; 6RW5; EM; 3.14 A; Q=2-87.
DR PDB; 6VLZ; EM; 2.97 A; AQ=1-87.
DR PDB; 6VMI; EM; 2.96 A; AQ=1-87.
DR PDB; 6ZM5; EM; 2.89 A; AQ=2-87.
DR PDB; 6ZM6; EM; 2.59 A; AQ=2-87.
DR PDB; 6ZS9; EM; 4.00 A; AQ=2-87.
DR PDB; 6ZSA; EM; 4.00 A; AQ=2-87.
DR PDB; 6ZSB; EM; 4.50 A; AQ=2-87.
DR PDB; 6ZSC; EM; 3.50 A; AQ=2-87.
DR PDB; 6ZSD; EM; 3.70 A; AQ=1-87.
DR PDB; 6ZSE; EM; 5.00 A; AQ=2-87.
DR PDB; 6ZSG; EM; 4.00 A; AQ=1-87.
DR PDB; 7A5F; EM; 4.40 A; Q6=1-87.
DR PDB; 7A5G; EM; 4.33 A; Q6=1-87.
DR PDB; 7A5I; EM; 3.70 A; Q6=1-87.
DR PDB; 7A5K; EM; 3.70 A; Q6=1-87.
DR PDB; 7L08; EM; 3.49 A; AQ=1-87.
DR PDB; 7OG4; EM; 3.80 A; AQ=1-87.
DR PDBsum; 3J9M; -.
DR PDBsum; 6NU2; -.
DR PDBsum; 6NU3; -.
DR PDBsum; 6RW4; -.
DR PDBsum; 6RW5; -.
DR PDBsum; 6VLZ; -.
DR PDBsum; 6VMI; -.
DR PDBsum; 6ZM5; -.
DR PDBsum; 6ZM6; -.
DR PDBsum; 6ZS9; -.
DR PDBsum; 6ZSA; -.
DR PDBsum; 6ZSB; -.
DR PDBsum; 6ZSC; -.
DR PDBsum; 6ZSD; -.
DR PDBsum; 6ZSE; -.
DR PDBsum; 6ZSG; -.
DR PDBsum; 7A5F; -.
DR PDBsum; 7A5G; -.
DR PDBsum; 7A5I; -.
DR PDBsum; 7A5K; -.
DR PDBsum; 7L08; -.
DR PDBsum; 7OG4; -.
DR AlphaFoldDB; P82921; -.
DR SMR; P82921; -.
DR BioGRID; 119966; 140.
DR ComplexPortal; CPX-5225; 28S mitochondrial small ribosomal subunit.
DR CORUM; P82921; -.
DR IntAct; P82921; 37.
DR MINT; P82921; -.
DR STRING; 9606.ENSP00000461930; -.
DR iPTMnet; P82921; -.
DR MetOSite; P82921; -.
DR PhosphoSitePlus; P82921; -.
DR SwissPalm; P82921; -.
DR BioMuta; MRPS21; -.
DR EPD; P82921; -.
DR jPOST; P82921; -.
DR MassIVE; P82921; -.
DR PaxDb; P82921; -.
DR PeptideAtlas; P82921; -.
DR PRIDE; P82921; -.
DR ProteomicsDB; 57721; -.
DR Antibodypedia; 74489; 116 antibodies from 22 providers.
DR DNASU; 54460; -.
DR Ensembl; ENST00000581066.2; ENSP00000461930.1; ENSG00000266472.6.
DR Ensembl; ENST00000614145.5; ENSP00000480129.1; ENSG00000266472.6.
DR GeneID; 54460; -.
DR KEGG; hsa:54460; -.
DR MANE-Select; ENST00000614145.5; ENSP00000480129.1; NM_031901.6; NP_114107.2.
DR UCSC; uc001euk.5; human.
DR CTD; 54460; -.
DR DisGeNET; 54460; -.
DR GeneCards; MRPS21; -.
DR HGNC; HGNC:14046; MRPS21.
DR HPA; ENSG00000266472; Low tissue specificity.
DR MIM; 611984; gene.
DR neXtProt; NX_P82921; -.
DR OpenTargets; ENSG00000266472; -.
DR PharmGKB; PA31009; -.
DR VEuPathDB; HostDB:ENSG00000266472; -.
DR eggNOG; ENOG502S4BS; Eukaryota.
DR GeneTree; ENSGT00500000045038; -.
DR InParanoid; P82921; -.
DR OMA; MRHAQFL; -.
DR OrthoDB; 1512634at2759; -.
DR PhylomeDB; P82921; -.
DR TreeFam; TF313903; -.
DR PathwayCommons; P82921; -.
DR Reactome; R-HSA-5368286; Mitochondrial translation initiation.
DR Reactome; R-HSA-5389840; Mitochondrial translation elongation.
DR Reactome; R-HSA-5419276; Mitochondrial translation termination.
DR SignaLink; P82921; -.
DR SIGNOR; P82921; -.
DR BioGRID-ORCS; 54460; 678 hits in 1048 CRISPR screens.
DR ChiTaRS; MRPS21; human.
DR GeneWiki; MRPS21; -.
DR GenomeRNAi; 54460; -.
DR Pharos; P82921; Tdark.
DR PRO; PR:P82921; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; P82921; protein.
DR Bgee; ENSG00000266472; Expressed in cardiac muscle of right atrium and 188 other tissues.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0003735; F:structural constituent of ribosome; ISS:UniProtKB.
DR GO; GO:0032543; P:mitochondrial translation; ISS:UniProtKB.
DR GO; GO:0006412; P:translation; NAS:UniProtKB.
DR Gene3D; 1.20.5.1150; -; 1.
DR InterPro; IPR001911; Ribosomal_S21.
DR InterPro; IPR038380; S21_sf.
DR PANTHER; PTHR21109; PTHR21109; 1.
DR Pfam; PF01165; Ribosomal_S21; 1.
DR TIGRFAMs; TIGR00030; S21p; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Mitochondrion; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein.
FT CHAIN 1..87
FT /note="28S ribosomal protein S21, mitochondrial"
FT /id="PRO_0000178413"
FT VARIANT 50
FT /note="C -> R (in dbSNP:rs10480)"
FT /evidence="ECO:0000269|PubMed:11402041,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:16710414, ECO:0000269|Ref.2,
FT ECO:0000269|Ref.5"
FT /id="VAR_051825"
FT VARIANT 51
FT /note="R -> Q (in dbSNP:rs4845)"
FT /id="VAR_024610"
SQ SEQUENCE 87 AA; 10689 MW; BCFAEB58D9F7ECAA CRC64;
MAKHLKFIAR TVMVQEGNVE SAYRTLNRIL TMDGLIEDIK HRRYYEKPCC RRQRESYERC
RRIYNMEMAR KINFLMRKNR ADPWQGC
