ID   RT2OK_NIDOK             Reviewed;          30 AA.
AC   C0HL07;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2018, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Ranatuerin-2OK {ECO:0000303|PubMed:15629529};
OS   Nidirana okinavana (Kampira Falls frog) (Babina okinavana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Nidirana.
OX   NCBI_TaxID=156870 {ECO:0000303|PubMed:15629529};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND DISULFIDE BOND.
RC   TISSUE=Skin {ECO:0000303|PubMed:15629529};
RX   PubMed=15629529; DOI=10.1016/j.peptides.2004.08.008;
RA   Conlon J.M., Sonnevend A., Jouenne T., Coquet L., Cosquer D., Vaudry H.,
RA   Iwamuro S.;
RT   "A family of acyclic brevinin-1 peptides from the skin of the Ryukyu brown
RT   frog Rana okinavana.";
RL   Peptides 26:185-190(2005).
CC   -!- FUNCTION: Antimicrobial peptide. Active against Gram-negative bacterium
CC       E.coli (MIC=12.5 uM) and against Gram-positive bacterium S.aureus
CC       (MIC=50 uM). {ECO:0000269|PubMed:15629529}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P86161}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:15629529}.
CC   -!- MASS SPECTROMETRY: Mass=3183.7; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15629529};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; C0HL07; -.
DR   SMR; C0HL07; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Secreted.
FT   PEPTIDE         1..30
FT                   /note="Ranatuerin-2OK"
FT                   /evidence="ECO:0000269|PubMed:15629529"
FT                   /id="PRO_0000443441"
FT   DISULFID        23..30
FT                   /evidence="ECO:0000269|PubMed:15629529"
SQ   SEQUENCE   30 AA;  3188 MW;  80E7BB37FA458FDE CRC64;
     SFLNFFKGAA KNLLAAGLDK LKCKISGTQC