RT33_HUMAN
ID RT33_HUMAN Reviewed; 106 AA.
AC Q9Y291;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=28S ribosomal protein S33, mitochondrial;
DE Short=MRP-S33;
DE Short=S33mt;
DE AltName: Full=Mitochondrial small ribosomal subunit protein mS33 {ECO:0000303|PubMed:25838379};
GN Name=MRPS33; ORFNames=CGI-139, PTD003;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2] {ECO:0000312|EMBL:AAH15462.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pituitary tumor;
RA Dai M., Peng Y., Song H., Huang Q., Mao Y., Zhang Q., Mao M., Fu G.,
RA Luo M., Chen J., Hu R.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=11279123; DOI=10.1074/jbc.m100727200;
RA Koc E.C., Burkhart W., Blackburn K., Moseley A., Spremulli L.L.;
RT "The small subunit of the mammalian mitochondrial ribosome: identification
RT of the full complement of ribosomal proteins present.";
RL J. Biol. Chem. 276:19363-19374(2001).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [11]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS), AND SUBUNIT.
RX PubMed=25838379; DOI=10.1126/science.aaa1193;
RA Amunts A., Brown A., Toots J., Scheres S.H., Ramakrishnan V.;
RT "Ribosome. The structure of the human mitochondrial ribosome.";
RL Science 348:95-98(2015).
CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-
CC SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small
CC (28S) and a large (39S) subunit. The 28S small subunit contains a 12S
CC ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large
CC subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial
CC valine transfer RNA (mt-tRNA(Val)), which plays an integral structural
CC role, and 52 different proteins. {ECO:0000269|PubMed:25838379}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:11279123}.
CC -!- SIMILARITY: Belongs to the mitochondrion-specific ribosomal protein
CC mS33 family. {ECO:0000305}.
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DR EMBL; AF151897; AAD34134.1; -; mRNA.
DR EMBL; AF078858; AAD44490.1; -; mRNA.
DR EMBL; AC006006; AAD43190.1; -; Genomic_DNA.
DR EMBL; BC015462; AAH15462.1; -; mRNA.
DR CCDS; CCDS5864.1; -.
DR RefSeq; NP_057155.1; NM_016071.3.
DR RefSeq; NP_444263.1; NM_053035.2.
DR PDB; 3J9M; EM; 3.50 A; AZ=1-106.
DR PDB; 6NU2; EM; 3.90 A; AZ=5-91.
DR PDB; 6NU3; EM; 4.40 A; AZ=1-106.
DR PDB; 6RW4; EM; 2.97 A; Z=1-106.
DR PDB; 6RW5; EM; 3.14 A; Z=1-106.
DR PDB; 6VLZ; EM; 2.97 A; AZ=1-106.
DR PDB; 6VMI; EM; 2.96 A; AZ=1-106.
DR PDB; 6ZM5; EM; 2.89 A; AZ=1-106.
DR PDB; 6ZM6; EM; 2.59 A; AZ=1-106.
DR PDB; 6ZS9; EM; 4.00 A; AZ=1-106.
DR PDB; 6ZSA; EM; 4.00 A; AZ=1-106.
DR PDB; 6ZSB; EM; 4.50 A; AZ=1-106.
DR PDB; 6ZSC; EM; 3.50 A; AZ=1-106.
DR PDB; 6ZSD; EM; 3.70 A; AZ=1-106.
DR PDB; 6ZSE; EM; 5.00 A; AZ=1-106.
DR PDB; 6ZSG; EM; 4.00 A; AZ=1-106.
DR PDB; 7A5F; EM; 4.40 A; Z6=1-106.
DR PDB; 7A5G; EM; 4.33 A; Z6=1-106.
DR PDB; 7A5I; EM; 3.70 A; Z6=1-106.
DR PDB; 7A5K; EM; 3.70 A; Z6=1-106.
DR PDB; 7L08; EM; 3.49 A; AZ=1-106.
DR PDB; 7OG4; EM; 3.80 A; AZ=1-106.
DR PDBsum; 3J9M; -.
DR PDBsum; 6NU2; -.
DR PDBsum; 6NU3; -.
DR PDBsum; 6RW4; -.
DR PDBsum; 6RW5; -.
DR PDBsum; 6VLZ; -.
DR PDBsum; 6VMI; -.
DR PDBsum; 6ZM5; -.
DR PDBsum; 6ZM6; -.
DR PDBsum; 6ZS9; -.
DR PDBsum; 6ZSA; -.
DR PDBsum; 6ZSB; -.
DR PDBsum; 6ZSC; -.
DR PDBsum; 6ZSD; -.
DR PDBsum; 6ZSE; -.
DR PDBsum; 6ZSG; -.
DR PDBsum; 7A5F; -.
DR PDBsum; 7A5G; -.
DR PDBsum; 7A5I; -.
DR PDBsum; 7A5K; -.
DR PDBsum; 7L08; -.
DR PDBsum; 7OG4; -.
DR AlphaFoldDB; Q9Y291; -.
DR SMR; Q9Y291; -.
DR BioGRID; 119658; 155.
DR ComplexPortal; CPX-5225; 28S mitochondrial small ribosomal subunit.
DR CORUM; Q9Y291; -.
DR IntAct; Q9Y291; 42.
DR MINT; Q9Y291; -.
DR STRING; 9606.ENSP00000376732; -.
DR iPTMnet; Q9Y291; -.
DR PhosphoSitePlus; Q9Y291; -.
DR BioMuta; MRPS33; -.
DR DMDM; 24212388; -.
DR EPD; Q9Y291; -.
DR jPOST; Q9Y291; -.
DR MassIVE; Q9Y291; -.
DR MaxQB; Q9Y291; -.
DR PaxDb; Q9Y291; -.
DR PeptideAtlas; Q9Y291; -.
DR PRIDE; Q9Y291; -.
DR ProteomicsDB; 85701; -.
DR TopDownProteomics; Q9Y291; -.
DR Antibodypedia; 32470; 117 antibodies from 21 providers.
DR DNASU; 51650; -.
DR Ensembl; ENST00000324787.10; ENSP00000320567.5; ENSG00000090263.16.
DR Ensembl; ENST00000393008.7; ENSP00000376732.3; ENSG00000090263.16.
DR Ensembl; ENST00000469351.1; ENSP00000417287.1; ENSG00000090263.16.
DR Ensembl; ENST00000496958.5; ENSP00000418451.1; ENSG00000090263.16.
DR GeneID; 51650; -.
DR KEGG; hsa:51650; -.
DR MANE-Select; ENST00000324787.10; ENSP00000320567.5; NM_053035.3; NP_444263.1.
DR UCSC; uc003vwd.5; human.
DR CTD; 51650; -.
DR DisGeNET; 51650; -.
DR GeneCards; MRPS33; -.
DR HGNC; HGNC:16634; MRPS33.
DR HPA; ENSG00000090263; Low tissue specificity.
DR MIM; 611993; gene.
DR neXtProt; NX_Q9Y291; -.
DR OpenTargets; ENSG00000090263; -.
DR PharmGKB; PA31020; -.
DR VEuPathDB; HostDB:ENSG00000090263; -.
DR eggNOG; KOG4104; Eukaryota.
DR GeneTree; ENSGT00390000011401; -.
DR InParanoid; Q9Y291; -.
DR OMA; EVYSWYP; -.
DR OrthoDB; 1612570at2759; -.
DR PhylomeDB; Q9Y291; -.
DR TreeFam; TF313928; -.
DR PathwayCommons; Q9Y291; -.
DR Reactome; R-HSA-5368286; Mitochondrial translation initiation.
DR Reactome; R-HSA-5389840; Mitochondrial translation elongation.
DR Reactome; R-HSA-5419276; Mitochondrial translation termination.
DR SignaLink; Q9Y291; -.
DR SIGNOR; Q9Y291; -.
DR BioGRID-ORCS; 51650; 142 hits in 1047 CRISPR screens.
DR ChiTaRS; MRPS33; human.
DR GeneWiki; MRPS33; -.
DR GenomeRNAi; 51650; -.
DR Pharos; Q9Y291; Tdark.
DR PRO; PR:Q9Y291; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9Y291; protein.
DR Bgee; ENSG00000090263; Expressed in mucosa of transverse colon and 200 other tissues.
DR ExpressionAtlas; Q9Y291; baseline and differential.
DR Genevisible; Q9Y291; HS.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; NAS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; NAS:UniProtKB.
DR GO; GO:0032543; P:mitochondrial translation; IC:ComplexPortal.
DR GO; GO:0006412; P:translation; NAS:UniProtKB.
DR InterPro; IPR013219; Ribosomal_S27/S33_mit.
DR PANTHER; PTHR13362; PTHR13362; 1.
DR Pfam; PF08293; MRP-S33; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Mitochondrion; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895,
FT ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712"
FT CHAIN 2..106
FT /note="28S ribosomal protein S33, mitochondrial"
FT /id="PRO_0000087727"
FT REGION 82..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..106
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22223895,
FT ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712"
SQ SEQUENCE 106 AA; 12629 MW; E755EDAFB6AF6A46 CRC64;
MSSLSEYAFR MSRLSARLFG EVTRPTNSKS MKVVKLFSEL PLAKKKETYD WYPNHHTYAE
LMQTLRFLGL YRDEHQDFMD EQKRLKKLRG KEKPKKGEGK RAAKRK
