RT34_MOUSE
ID RT34_MOUSE Reviewed; 218 AA.
AC Q9JIK9; Q9D2B0; Q9D957;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=28S ribosomal protein S34, mitochondrial;
DE Short=MRP-S34;
DE Short=S34mt;
DE AltName: Full=T-complex expressed gene 2 protein;
GN Name=Mrps34; Synonyms=Tce2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=10899141; DOI=10.1101/gr.10.7.916;
RA Kargul G.J., Nagaraja R., Shimada T., Grahovac M.J., Lim M.K.,
RA Nakashima H., Waeltz P., Ma P., Chen E., Schlessinger D., Ko M.S.H.;
RT "Eleven densely clustered genes, six of them novel, in 176 kb of mouse T-
RT complex DNA.";
RL Genome Res. 10:916-923(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Kidney, Pancreas, and Pituitary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, MUTAGENESIS OF LEU-68, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=25816300; DOI=10.1371/journal.pgen.1005089;
RA Richman T.R., Ermer J.A., Davies S.M., Perks K.L., Viola H.M.,
RA Shearwood A.M., Hool L.C., Rackham O., Filipovska A.;
RT "Mutation in MRPS34 compromises protein synthesis and causes mitochondrial
RT dysfunction.";
RL PLoS Genet. 11:E1005089-E1005089(2015).
CC -!- FUNCTION: Required for mitochondrial translation, plays a role in
CC maintaining the stability of the small ribosomal subunit and the 12S
CC rRNA that are required for mitoribosome formation.
CC {ECO:0000269|PubMed:25816300}.
CC -!- SUBUNIT: Component of the mitochondrial ribosome small subunit (28S)
CC which comprises a 12S rRNA and about 30 distinct proteins.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:25816300}.
CC -!- TISSUE SPECIFICITY: Widely expressed (at protein liver).
CC {ECO:0000269|PubMed:25816300}.
CC -!- SIMILARITY: Belongs to the mitochondrion-specific ribosomal protein
CC mS34 family. {ECO:0000305}.
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DR EMBL; AF220294; AAF69478.1; -; Genomic_DNA.
DR EMBL; AK002285; BAB21988.1; -; mRNA.
DR EMBL; AK004049; BAB23142.1; -; mRNA.
DR EMBL; AK007337; BAB24970.1; -; mRNA.
DR EMBL; AK019922; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC024336; AAH24336.1; -; mRNA.
DR CCDS; CCDS28502.1; -.
DR RefSeq; NP_075749.1; NM_023260.1.
DR AlphaFoldDB; Q9JIK9; -.
DR SMR; Q9JIK9; -.
DR BioGRID; 219729; 5.
DR ComplexPortal; CPX-5301; 28S mitochondrial small ribosomal subunit.
DR STRING; 10090.ENSMUSP00000045111; -.
DR iPTMnet; Q9JIK9; -.
DR PhosphoSitePlus; Q9JIK9; -.
DR SwissPalm; Q9JIK9; -.
DR EPD; Q9JIK9; -.
DR jPOST; Q9JIK9; -.
DR MaxQB; Q9JIK9; -.
DR PaxDb; Q9JIK9; -.
DR PeptideAtlas; Q9JIK9; -.
DR PRIDE; Q9JIK9; -.
DR ProteomicsDB; 260863; -.
DR Antibodypedia; 23234; 309 antibodies from 27 providers.
DR DNASU; 79044; -.
DR Ensembl; ENSMUST00000043907; ENSMUSP00000045111; ENSMUSG00000038880.
DR Ensembl; ENSMUST00000154236; ENSMUSP00000120985; ENSMUSG00000038880.
DR GeneID; 79044; -.
DR KEGG; mmu:79044; -.
DR UCSC; uc008ayy.1; mouse.
DR CTD; 65993; -.
DR MGI; MGI:1930188; Mrps34.
DR VEuPathDB; HostDB:ENSMUSG00000038880; -.
DR eggNOG; ENOG502QSI0; Eukaryota.
DR GeneTree; ENSGT00390000008964; -.
DR HOGENOM; CLU_116794_0_0_1; -.
DR InParanoid; Q9JIK9; -.
DR OMA; IPKHMEQ; -.
DR OrthoDB; 1475824at2759; -.
DR PhylomeDB; Q9JIK9; -.
DR TreeFam; TF320386; -.
DR Reactome; R-MMU-5389840; Mitochondrial translation elongation.
DR Reactome; R-MMU-5419276; Mitochondrial translation termination.
DR BioGRID-ORCS; 79044; 25 hits in 75 CRISPR screens.
DR ChiTaRS; Mrps34; mouse.
DR PRO; PR:Q9JIK9; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9JIK9; protein.
DR Bgee; ENSMUSG00000038880; Expressed in proximal tubule and 128 other tissues.
DR Genevisible; Q9JIK9; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003735; F:structural constituent of ribosome; ISO:MGI.
DR GO; GO:0032543; P:mitochondrial translation; IMP:UniProtKB.
DR InterPro; IPR032053; MRP-S34.
DR PANTHER; PTHR28589; PTHR28589; 1.
DR Pfam; PF16053; MRP-S34; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Reference proteome; Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..218
FT /note="28S ribosomal protein S34, mitochondrial"
FT /id="PRO_0000087731"
FT MUTAGEN 68
FT /note="L->P: Decreases protein expression. Decreases
FT mitochondrial ribosomal subunits and translationally
FT competent mitoribosomes. Mutant mice show heart hypertrophy
FT and liver steatosis."
FT /evidence="ECO:0000269|PubMed:25816300"
FT CONFLICT 5
FT /note="K -> E (in Ref. 2; BAB24970)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 218 AA; 25827 MW; 1C8D03A1F13BD85B CRC64;
MARKKVRPRL IAELARRVRA LREQRNQPRD SQLYALDYET LTRPHSGRRL PVRAWADVRR
ESRLLQLLAR LPLFGLGRLV TRKSWLWQHD EPCYWRLTRV RPDYTAQNLD HGRAWGILTF
KGKSEDTARE IEQVMYHDWR LVPKHEEEAF TAFTAKPEDR LNSVPYPPLL RAMILAERQK
NGDTSVQEPL LNLERTRMRP WDYPAKQETK GRAKGTPV
