RT4I1_CAEEL
ID RT4I1_CAEEL Reviewed; 366 AA.
AC Q4W4Z2;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Reticulon-4-interacting protein 1, mitochondrial {ECO:0000250|UniProtKB:Q8WWV3};
DE EC=1.-.-.- {ECO:0000303|PubMed:21895890};
DE Flags: Precursor;
GN Name=rad-8 {ECO:0000312|WormBase:F56H1.6};
GN ORFNames=F56H1.6 {ECO:0000312|WormBase:F56H1.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=7152245; DOI=10.1093/genetics/102.2.159;
RA Hartman P.S., Herman R.K.;
RT "Radiation-sensitive mutants of Caenorhabditis elegans.";
RL Genetics 102:159-178(1982).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8350650; DOI=10.1016/0047-6374(93)90135-e;
RA Ishi N., Suzuki N., Hartman P.S., Suzuki K.;
RT "The radiation-sensitive mutant rad-8 of Caenorhabditis elegans is
RT hypersensitive to the effects of oxygen on aging and development.";
RL Mech. Ageing Dev. 68:1-10(1993).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8169328; DOI=10.1093/geronj/49.3.b117;
RA Ishii N., Suzuki N., Hartman P.S., Suzuki K.;
RT "The effects of temperature on the longevity of a radiation-sensitive
RT mutant rad-8 of the nematode Caenorhabditis elegans.";
RL J. Gerontol. 49:B117-B120(1994).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9989496; DOI=10.1016/s0092-8674(00)80958-7;
RA Mahajan-Miklos S., Tan M.-W., Rahme L.G., Ausubel F.M.;
RT "Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas
RT aeruginosa-Caenorhabditis elegans pathogenesis model.";
RL Cell 96:47-56(1999).
RN [6] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21895890; DOI=10.1111/j.1365-2443.2011.01547.x;
RA Fujii M., Yasuda K., Hartman P.S., Ayusawa D., Ishii N.;
RT "A mutation in a mitochondrial dehydrogenase/reductase gene causes an
RT increased sensitivity to oxidative stress and mitochondrial defects in the
RT nematode Caenorhabditis elegans.";
RL Genes Cells 16:1022-1034(2011).
CC -!- FUNCTION: Plays a role in oxygen metabolism in the mitochondria by
CC regulating the levels of reactive oxygen species (ROS) thereby
CC conferring resistance to oxidative stress (PubMed:7152245,
CC PubMed:8350650, PubMed:8169328, PubMed:21895890). Involved in
CC resistance to P.aeruginosa PA14 infection (PubMed:9989496). Regulates
CC lifespan (PubMed:8169328). {ECO:0000269|PubMed:21895890,
CC ECO:0000269|PubMed:7152245, ECO:0000269|PubMed:8169328,
CC ECO:0000269|PubMed:8350650, ECO:0000269|PubMed:9989496}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:21895890}.
CC -!- TISSUE SPECIFICITY: Expressed in pharynx, muscles and intestine.
CC {ECO:0000269|PubMed:21895890}.
CC -!- DISRUPTION PHENOTYPE: Under hyperoxic conditions or low temperature (16
CC degrees Celsius), lifespan, body size and fertility are severely
CC reduced and sexual maturity and egg hatching are delayed
CC (PubMed:8350650, PubMed:8169328). Slight increase in the number of
CC apoptotic cells at the embryonic comma stage (PubMed:21895890). Also
CC shows strong susceptibility to UV irradiation and paraquat treatment.
CC Does not show increased sensitivity to X-ray radiation (PubMed:7152245,
CC PubMed:8350650). Increased sensitivity to P.aeruginosa PA14-mediated
CC killing (PubMed:9989496). Body wall muscle mitochondria appear enlarged
CC and abnormal with less developed cristae in hypoxic or hyperoxic
CC conditions (PubMed:21895890). {ECO:0000269|PubMed:21895890,
CC ECO:0000269|PubMed:7152245, ECO:0000269|PubMed:8169328,
CC ECO:0000269|PubMed:8350650, ECO:0000269|PubMed:9989496}.
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. Quinone oxidoreductase subfamily. {ECO:0000305}.
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DR EMBL; BX284601; CCD62972.1; -; Genomic_DNA.
DR RefSeq; NP_001021512.2; NM_001026341.2.
DR AlphaFoldDB; Q4W4Z2; -.
DR SMR; Q4W4Z2; -.
DR STRING; 6239.F56H1.6; -.
DR EPD; Q4W4Z2; -.
DR PaxDb; Q4W4Z2; -.
DR PeptideAtlas; Q4W4Z2; -.
DR PRIDE; Q4W4Z2; -.
DR EnsemblMetazoa; F56H1.6.1; F56H1.6.1; WBGene00044305.
DR GeneID; 3565366; -.
DR KEGG; cel:CELE_F56H1.6; -.
DR UCSC; F56H1.6; c. elegans.
DR CTD; 3565366; -.
DR WormBase; F56H1.6; CE45632; WBGene00044305; rad-8.
DR eggNOG; KOG1198; Eukaryota.
DR GeneTree; ENSGT00880000138028; -.
DR HOGENOM; CLU_026673_3_3_1; -.
DR InParanoid; Q4W4Z2; -.
DR OMA; PCGATTS; -.
DR OrthoDB; 727365at2759; -.
DR PhylomeDB; Q4W4Z2; -.
DR PRO; PR:Q4W4Z2; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00044305; Expressed in adult organism and 3 other tissues.
DR GO; GO:0005739; C:mitochondrion; IDA:WormBase.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0045087; P:innate immune response; IMP:WormBase.
DR GO; GO:0032504; P:multicellular organism reproduction; IMP:WormBase.
DR GO; GO:0040010; P:positive regulation of growth rate; IMP:WormBase.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IMP:WormBase.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR GO; GO:0006979; P:response to oxidative stress; IMP:WormBase.
DR GO; GO:0070482; P:response to oxygen levels; IMP:WormBase.
DR GO; GO:0000303; P:response to superoxide; IMP:WormBase.
DR GO; GO:0009411; P:response to UV; IMP:WormBase.
DR CDD; cd08248; RTN4I1; 1.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR037397; RTN4I1.
DR Pfam; PF08240; ADH_N; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Mitochondrion; Oxidoreductase; Reference proteome; Stress response;
KW Transit peptide.
FT TRANSIT 1..20
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 21..366
FT /note="Reticulon-4-interacting protein 1, mitochondrial"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436534"
SQ SEQUENCE 366 AA; 40111 MW; 4B700B7A2EFCEC9A CRC64;
MIEKMILRRF FSTKSSTMRA WVSENGSGVE LKEVPLPVIN KPGQVLLKVK AASVNPIDVD
MSQGYGREFL GTWKKIESCD AAASRFPLIP GRDCTAVVES VGGDVHNLAP GDEVMAVVPV
ILPGTHAEFV VTDSKYCSKK PSNLSFVSAA ALPYVASTAY SAFTIARVSQ RNAKQQRVLI
HGGAGGVGSM AIQLLKAWGC EKIVATCAKG SFDIVKQLGA IPVDYTSDQA TQELIEHAPF
EVILDTVDSQ LAKWSDNVMG VWRNCVHVSI VSPLMREMDK NGVPLGLVTT AMKHFERSFQ
SHLRGRWFSY AFFRPSSDLM SQLSRFAEDG KIVPVVEQVM GFEELEKAYE KVSQLNGRGK
TVIKYD
