RT65_MYXXA
ID RT65_MYXXA Reviewed; 427 AA.
AC P23071;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Retron Mx65 reverse transcriptase {ECO:0000305};
DE Short=Mx65-RT;
DE EC=2.7.7.49 {ECO:0000255|PROSITE-ProRule:PRU00405};
DE AltName: Full=RNA-directed DNA polymerase from retron Mx65;
OS Myxococcus xanthus.
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC Cystobacterineae; Myxococcaceae; Myxococcus.
OX NCBI_TaxID=34;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DZF9;
RX PubMed=1689062; DOI=10.1073/pnas.87.3.942;
RA Inouye S., Herzer P.J., Inouye M.;
RT "Two independent retrons with highly diverse reverse transcriptases in
RT Myxococcus xanthus.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:942-945(1990).
CC -!- FUNCTION: Reverse transcriptase (RT) responsible for synthesis of
CC msDNA-Mx65 (a branched molecule with RNA linked by a 2',5'-
CC phosphodiester bond to ssDNA). The retron transcript serves as primer
CC (from a conserved internal G residue) and template for the reaction,
CC and codes for the RT (By similarity). The retron is involved in
CC antiviral defense (By similarity). {ECO:0000250|UniProtKB:P23072,
CC ECO:0000250|UniProtKB:P71276}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00405};
CC -!- MISCELLANEOUS: M.xanthus contains two independent and unlinked retrons:
CC Mx65 and Mx162. {ECO:0000269|PubMed:1689062}.
CC -!- MISCELLANEOUS: Retrons may be the ancestors of retrovirus.
CC -!- SIMILARITY: Belongs to the bacterial reverse transcriptase family.
CC {ECO:0000305}.
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DR EMBL; M30609; AAA88323.1; -; Genomic_DNA.
DR PIR; A34864; RRYC65.
DR AlphaFoldDB; P23071; -.
DR SMR; P23071; -.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR CDD; cd03487; RT_Bac_retron_II; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000123; Reverse_transcriptase_msDNA.
DR InterPro; IPR000477; RT_dom.
DR Pfam; PF00078; RVT_1; 1.
DR PRINTS; PR00866; RNADNAPOLMS.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 3: Inferred from homology;
KW Antiviral defense; Magnesium; Metal-binding; Nucleotidyltransferase;
KW RNA-directed DNA polymerase; Transferase; Transposable element.
FT CHAIN 1..427
FT /note="Retron Mx65 reverse transcriptase"
FT /id="PRO_0000097507"
FT DOMAIN 136..366
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT BINDING 219
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT BINDING 315
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT BINDING 316
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
SQ SEQUENCE 427 AA; 48025 MW; C96436BF86E4D0D7 CRC64;
MSWFDTTLSR LKGLFSRPVT RSTTGLDVPL DAHGRPQDVV TETVSTSGPL KPGHLRQVRR
DARLLPKGVR RYTPGRKKWM EAAEARRLFS ATLRTRNRNL RDLLPDEAQL ARYGLPVWRT
EEDVAAALGV SVGVLRHYSI HRPRERVRHY VTFAVPKRSG GVRLLHAPKR RLKALQRRML
ALLVSKLPVS PQAHGFVPGR SIKTGAAPHV GRRVVLKLDL KDFFPSVTFA RVRGLLIALG
YGYPVAATLA VLMTESERQP VELEGILFHV PVGPRVCVQG APTSPALCNA VLLRLDRRLA
GLARRYGYTY TRYADDLTFS GDDVTALERV RALAARYVQE EGFEVNREKT RVQRRGGAQR
VTGVTVNTTL GLSREERPRL RAMLHQEARS EDVEAHRAHL DGLLAYVKML NPEQAERLAR
RRKPRGT
