RTA1_YEAST
ID RTA1_YEAST Reviewed; 317 AA.
AC P53047; D6VUZ6; E9P922;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein RTA1;
GN Name=RTA1; OrderedLocusNames=YGR213C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX PubMed=8660468; DOI=10.1007/s002940050110;
RA Soustre I., Letourneux Y., Karst F.;
RT "Characterization of the Saccharomyces cerevisiae RTA1 gene involved in 7-
RT aminocholesterol resistance.";
RL Curr. Genet. 30:121-125(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Involved in 7-aminocholesterol resistance.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the lipid-translocating exporter (LTE) (TC
CC 9.A.26.1) family. {ECO:0000305}.
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DR EMBL; X84736; CAA59227.1; -; Genomic_DNA.
DR EMBL; Z72998; CAA97240.1; -; Genomic_DNA.
DR EMBL; AY693143; AAT93162.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08307.1; -; Genomic_DNA.
DR PIR; S64536; S64536.
DR RefSeq; NP_011729.1; NM_001181342.1.
DR AlphaFoldDB; P53047; -.
DR SMR; P53047; -.
DR BioGRID; 33466; 17.
DR DIP; DIP-1599N; -.
DR IntAct; P53047; 1.
DR MINT; P53047; -.
DR STRING; 4932.YGR213C; -.
DR TCDB; 9.A.26.1.1; the lipid-translocating exporter (lte) family.
DR PaxDb; P53047; -.
DR EnsemblFungi; YGR213C_mRNA; YGR213C; YGR213C.
DR GeneID; 853127; -.
DR KEGG; sce:YGR213C; -.
DR SGD; S000003445; RTA1.
DR VEuPathDB; FungiDB:YGR213C; -.
DR eggNOG; ENOG502QURG; Eukaryota.
DR GeneTree; ENSGT00940000176387; -.
DR HOGENOM; CLU_033465_3_1_1; -.
DR InParanoid; P53047; -.
DR OMA; ISKKWMT; -.
DR BioCyc; YEAST:G3O-30895-MON; -.
DR PRO; PR:P53047; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53047; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR InterPro; IPR007568; RTA1.
DR PANTHER; PTHR31465; PTHR31465; 1.
DR Pfam; PF04479; RTA1; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..317
FT /note="Protein RTA1"
FT /id="PRO_0000097510"
FT TOPO_DOM 1..16
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..99
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 234
FT /note="I -> V (in Ref. 5; AAT93162)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 317 AA; 35486 MW; D4CF78080D129B4A CRC64;
MAKDGFELYR YTPELGASIL FTVLFAVSGV AFVILLFHYS VKSKRRVGSL MKSQPVLRYY
GTVNLAGAYI PFIFGCFVEC VGFAFRCKSS KDTTLLNPYI IQTVFLLVSP TLYAASIYMI
FGRMATLLFA ENLMIMPARF NTTIFVIGDV GSLLLQAIGG AMMSKVTSAS SGSHLVTAGL
FIQIAFFGLF IINEVLFIFK MSKKPTNVSV RYGSWKYLNI ALLVNSFLIL IRSIVRAVEF
IQGYDGEIAS HEWYLYIFDG LPMFLLVLIF IVAFPLINIF RIHEESIQAQ QSARFDGTDY
PDVEVTSIEE DLASKSE
