ID   RTBS_DROME              Reviewed;         906 AA.
AC   Q95SX7;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Probable RNA-directed DNA polymerase from transposon BS;
DE            EC=2.7.7.49;
DE   AltName: Full=Reverse transcriptase;
GN   Name=RTase {ECO:0000312|FlyBase:FBgn0044506};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1] {ECO:0000312|PIR:S55543}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7753626; DOI=10.1093/nar/23.8.1354;
RA   Udomkit A., Forbes S., Dalgleish G., Finnegan D.J.;
RT   "BS a novel LINE-like element in Drosophila melanogaster.";
RL   Nucleic Acids Res. 23:1354-1358(1995).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:AAL25477.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley {ECO:0000312|EMBL:AAL25477.1};
RC   TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49;
CC         Evidence={ECO:0000250|UniProtKB:P21328, ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
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DR   EMBL; AY060438; AAL25477.1; -; mRNA.
DR   PIR; S55543; S55543.
DR   AlphaFoldDB; Q95SX7; -.
DR   SMR; Q95SX7; -.
DR   FlyBase; FBgn0044506; BS\RTase.
DR   PRO; PR:Q95SX7; -.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IMP:UniProtKB.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IMP:UniProtKB.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR000477; RT_dom.
DR   Pfam; PF14529; Exo_endo_phos_2; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   2: Evidence at transcript level;
KW   Nucleotidyltransferase; RNA-directed DNA polymerase; Transferase;
KW   Transposable element.
FT   CHAIN           1..906
FT                   /note="Probable RNA-directed DNA polymerase from transposon
FT                   BS"
FT                   /id="PRO_0000076305"
FT   DOMAIN          482..758
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
SQ   SEQUENCE   906 AA;  103874 MW;  C0FB1DF6366B4511 CRC64;
     MNPLKILIWN ANGISRKAKD VELFAHNKKV DILLVTELRL KRGETVKIYG YAYYPAYRPS
     LNNNSVGGVA VFVRTTLRHF PQRVIETRHI QLSSVKVATG LGDLQFSAIY CSPSTRIEER
     HFTDIIRACG QRYLVGGDWN ARHWLWGDTC NSPRGRELAE ALSVTGAKIL ATGSPTRYPY
     VPSHTPSCID FAVYHGIPDH LATITQSWDL DSDHLPLIIS IETDSTHVNP SPRLVTKHTD
     LLAFSRQLES LISLNTTLNS GEEIEMAVDN LTESIHRAAA VSTSPVPRIG TTYGIVLTRE
     ARELLTQKRR LRRRAIRSQD PWDRLLWNRA AKQLRNVLRE LRSNFFEQKL ASMDYTVDAG
     YSLWKCTKSL KRQPFRQVPI RCPGGELAKN EEEQANCFAN HLETRFTHFQ FATTEQYQET
     LDSLETPLQM SLPIKPIRVE EIVEAIKSLP LKKSPGIDNV CNATLKALPV RAILYLALIY
     NAILRVQFFP KQWKMAAILM IHKPGKPEES PESYRPISLL SSLSKLWERL IANRLNDIMT
     ERRILPDHQF GFRQGHSTVE QVHRLTKHIL QAFDDKEYCN AVFIDMQQAF DRVWHDGLIS
     KVKKLFPAPY YGVLKSYLED RRFMVRVRNS YSIPRVMRAG VPQGSVLGPL LYSVFTADLP
     CPNAYHMADP RKALLATYAD DIALLYSSNC CNEAARGLQE YLTTLAAWCK RWILKVNPQK
     TINPCFTLKT LSPVTAPIEL EGVILDQPSQ AKYLGITLDK RLTFGPHLKA TTRRCYQRMQ
     QLRWLLNRKS TMTLRAKRAV YVHCVAPIWL YGIQIWGIAA KSNYNRIQVL QNRAMRAITD
     CPYYVRGTTL HRDLNLHTVE EQISRHTSRY SDRLRRHHSI LARRLLPARP LRRLKRKGFA
     KTLGQP