RTC6_YEAST
ID RTC6_YEAST Reviewed; 93 AA.
AC O14464; D6W3I5;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=54S ribosomal protein RTC6, mitochondrial;
DE AltName: Full=Mitochondrial large ribosomal subunit protein bL36m {ECO:0000303|PubMed:24675956};
DE AltName: Full=Restriction of telomere capping protein 6;
DE AltName: Full=Translation associated element 4;
DE Flags: Precursor;
GN Name=RTC6; Synonyms=TAE4; OrderedLocusNames=YPL183W-A; ORFNames=YPL183BW;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP PREDICTION OF FUNCTION.
RX PubMed=9392081;
RX DOI=10.1002/(sici)1097-0061(199711)13:14<1363::aid-yea182>3.0.co;2-8;
RA Andrade M.A., Daruvar A., Casari G., Schneider R., Termier M., Sander C.;
RT "Characterization of new proteins found by analysis of short open reading
RT frames from the full yeast genome.";
RL Yeast 13:1363-1374(1997).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=18845848; DOI=10.1534/genetics.108.092577;
RA Addinall S.G., Downey M., Yu M., Zubko M.K., Dewar J., Leake A.,
RA Hallinan J., Shaw O., James K., Wilkinson D.J., Wipat A., Durocher D.,
RA Lydall D.;
RT "A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied
RT cellular processes influencing telomere capping in Saccharomyces
RT cerevisiae.";
RL Genetics 180:2251-2266(2008).
RN [8]
RP FUNCTION.
RX PubMed=20691087; DOI=10.1186/1472-6769-10-6;
RA Alamgir M., Erukova V., Jessulat M., Azizi A., Golshani A.;
RT "Chemical-genetic profile analysis of five inhibitory compounds in yeast.";
RL BMC Chem. Biol. 10:6-6(2010).
RN [9]
RP SUBCELLULAR LOCATION.
RX PubMed=25609543; DOI=10.1038/ncomms7019;
RA Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT "Organization of the mitochondrial translation machinery studied in situ by
RT cryoelectron tomography.";
RL Nat. Commun. 6:6019-6019(2015).
RN [10]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX PubMed=24675956; DOI=10.1126/science.1249410;
RA Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., Long F.,
RA Murshudov G., Scheres S.H., Ramakrishnan V.;
RT "Structure of the yeast mitochondrial large ribosomal subunit.";
RL Science 343:1485-1489(2014).
CC -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC mitochondrial genome-encoded proteins, including at least some of the
CC essential transmembrane subunits of the mitochondrial respiratory
CC chain. The mitoribosomes are attached to the mitochondrial inner
CC membrane and translation products are cotranslationally integrated into
CC the membrane (PubMed:25609543, PubMed:24675956). bL36m may be involved
CC in a process influencing telomere capping (PubMed:18845848,
CC PubMed:20691087). {ECO:0000269|PubMed:18845848,
CC ECO:0000269|PubMed:20691087, ECO:0000305|PubMed:24675956,
CC ECO:0000305|PubMed:25609543}.
CC -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC and a large (54S) subunit. The 37S small subunit contains a 15S
CC ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC bL36m has a zinc binding site. {ECO:0000269|PubMed:24675956}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095}.
CC Note=Mitoribosomes are tethered to the mitochondrial inner membrane and
CC spatially aligned with the membrane insertion machinery through two
CC distinct membrane contact sites, formed by the 21S rRNA expansion
CC segment 96-ES1 and the inner membrane protein MBA1.
CC {ECO:0000269|PubMed:25609543}.
CC -!- MISCELLANEOUS: Present with 500 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL36 family.
CC {ECO:0000305}.
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DR EMBL; Z73540; CAA97895.1; -; Genomic_DNA.
DR EMBL; Z73539; CAA97893.1; -; Genomic_DNA.
DR EMBL; AY558522; AAS56848.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11251.1; -; Genomic_DNA.
DR PIR; S72254; S72254.
DR RefSeq; NP_015141.1; NM_001184317.1.
DR PDB; 3J6B; EM; 3.20 A; 0=1-93.
DR PDB; 5MRC; EM; 3.25 A; 0=56-93.
DR PDB; 5MRE; EM; 3.75 A; 0=56-93.
DR PDB; 5MRF; EM; 4.97 A; 0=56-93.
DR PDBsum; 3J6B; -.
DR PDBsum; 5MRC; -.
DR PDBsum; 5MRE; -.
DR PDBsum; 5MRF; -.
DR AlphaFoldDB; O14464; -.
DR SMR; O14464; -.
DR BioGRID; 35999; 270.
DR ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR DIP; DIP-6800N; -.
DR IntAct; O14464; 4.
DR STRING; 4932.YPL183W-A; -.
DR PaxDb; O14464; -.
DR PRIDE; O14464; -.
DR EnsemblFungi; YPL183W-A_mRNA; YPL183W-A; YPL183W-A.
DR GeneID; 855918; -.
DR KEGG; sce:YPL183W-A; -.
DR SGD; S000007224; RTC6.
DR VEuPathDB; FungiDB:YPL183W-A; -.
DR eggNOG; KOG4122; Eukaryota.
DR HOGENOM; CLU_135723_1_2_1; -.
DR InParanoid; O14464; -.
DR BioCyc; YEAST:G3O-34348-MON; -.
DR PRO; PR:O14464; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; O14464; protein.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; ISA:SGD.
DR GO; GO:0003735; F:structural constituent of ribosome; ISA:SGD.
DR GO; GO:0032543; P:mitochondrial translation; ISA:SGD.
DR GO; GO:0042254; P:ribosome biogenesis; IMP:SGD.
DR HAMAP; MF_00251; Ribosomal_L36; 1.
DR InterPro; IPR000473; Ribosomal_L36.
DR InterPro; IPR035977; Ribosomal_L36_sp.
DR Pfam; PF00444; Ribosomal_L36; 1.
DR SUPFAM; SSF57840; SSF57840; 1.
DR TIGRFAMs; TIGR01022; rpmJ_bact; 1.
DR PROSITE; PS00828; RIBOSOMAL_L36; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Mitochondrion; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..93
FT /note="54S ribosomal protein RTC6, mitochondrial"
FT /id="PRO_0000030531"
SQ SEQUENCE 93 AA; 10698 MW; A26DF14CA6E2F645 CRC64;
MFLQTLRLTM PRMFLHMKPS PITITRACTV PSLLSVAAPQ PALVAANRPL VFNRGFKVRT
SVKKFCSDCY LVRRKGRVYI YCKSNKKHKQ RQG
