RTEL1_CAEEL
ID RTEL1_CAEEL Reviewed; 994 AA.
AC Q93575; P90854; P90855;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Regulator of telomere elongation helicase 1 homolog {ECO:0000255|HAMAP-Rule:MF_03065};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_03065};
GN Name=rtel-1 {ECO:0000255|HAMAP-Rule:MF_03065};
GN Synonyms=bch-1 {ECO:0000255|HAMAP-Rule:MF_03065}; ORFNames=F25H2.13;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18957201; DOI=10.1016/j.cell.2008.08.016;
RA Barber L.J., Youds J.L., Ward J.D., McIlwraith M.J., O'Neil N.J.,
RA Petalcorin M.I.R., Martin J.S., Collis S.J., Cantor S.B., Auclair M.,
RA Tissenbaum H., West S.C., Rose A.M., Boulton S.J.;
RT "RTEL1 maintains genomic stability by suppressing homologous
RT recombination.";
RL Cell 135:261-271(2008).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20203049; DOI=10.1126/science.1183112;
RA Youds J.L., Mets D.G., McIlwraith M.J., Martin J.S., Ward J.D., Oneil N.J.,
RA Rose A.M., West S.C., Meyer B.J., Boulton S.J.;
RT "RTEL-1 enforces meiotic crossover interference and homeostasis.";
RL Science 327:1254-1258(2010).
CC -!- FUNCTION: ATP-dependent DNA helicase implicated in DNA repair and the
CC maintenance of genomic stability. Acts as an anti-recombinase to
CC counteract toxic recombination and limit crossover during meiosis.
CC Regulates meiotic recombination and crossover homeostasis by physically
CC dissociating strand invasion events and thereby promotes noncrossover
CC repair by meiotic synthesis dependent strand annealing (SDSA) as well
CC as disassembly of D loop recombination intermediates.
CC {ECO:0000255|HAMAP-Rule:MF_03065, ECO:0000269|PubMed:18957201,
CC ECO:0000269|PubMed:20203049}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03065};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03065}.
CC -!- DISRUPTION PHENOTYPE: Viable, although the brood size is reduced and
CC the life cycle is retarded (at 20 degrees Celsius, worms take 24 hours
CC longer to reach the gravid adult stage). The smaller brood size may
CC result from replicative stress, which is often associated with a
CC persistence of unrepaired DNA damage during development. Mutants show
CC defective crossover interference with all double-strand breaks becoming
CC crossovers and a compromised homeostasis after ionizing radiation.
CC Synthetic lethality in worms lacking both rtel-1 and him-6, with a
CC marked increase in the number of foci representing unresolved
CC recombination intermediates. {ECO:0000269|PubMed:18957201,
CC ECO:0000269|PubMed:20203049}.
CC -!- SIMILARITY: Belongs to the helicase family. RAD3/XPD subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_03065}.
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DR EMBL; Z79754; CAB02102.1; -; Genomic_DNA.
DR EMBL; Z83224; CAB02102.1; JOINED; Genomic_DNA.
DR PIR; T21356; T21356.
DR RefSeq; NP_492769.1; NM_060368.4.
DR AlphaFoldDB; Q93575; -.
DR SMR; Q93575; -.
DR BioGRID; 38360; 2.
DR IntAct; Q93575; 2.
DR MINT; Q93575; -.
DR STRING; 6239.F25H2.13.2; -.
DR EPD; Q93575; -.
DR PaxDb; Q93575; -.
DR PRIDE; Q93575; -.
DR EnsemblMetazoa; F25H2.13.1; F25H2.13.1; WBGene00009124.
DR EnsemblMetazoa; F25H2.13.2; F25H2.13.2; WBGene00009124.
DR GeneID; 172946; -.
DR KEGG; cel:CELE_F25H2.13; -.
DR UCSC; F25H2.13; c. elegans.
DR CTD; 172946; -.
DR WormBase; F25H2.13; CE15894; WBGene00009124; rtel-1.
DR eggNOG; KOG1132; Eukaryota.
DR GeneTree; ENSGT00950000182970; -.
DR HOGENOM; CLU_006515_4_0_1; -.
DR InParanoid; Q93575; -.
DR OMA; GNCATIV; -.
DR OrthoDB; 186062at2759; -.
DR PhylomeDB; Q93575; -.
DR PRO; PR:Q93575; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00009124; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; ISS:UniProtKB.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; ISS:UniProtKB.
DR GO; GO:0070182; F:DNA polymerase binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR GO; GO:0045910; P:negative regulation of DNA recombination; IBA:GO_Central.
DR GO; GO:1904430; P:negative regulation of t-circle formation; IBA:GO_Central.
DR GO; GO:0010569; P:regulation of double-strand break repair via homologous recombination; IGI:UniProtKB.
DR GO; GO:0090657; P:telomeric loop disassembly; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_03065; RTEL1; 1.
DR InterPro; IPR006555; ATP-dep_Helicase_C.
DR InterPro; IPR010614; DEAD_2.
DR InterPro; IPR045028; DinG/Rad3-like.
DR InterPro; IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
DR InterPro; IPR006554; Helicase-like_DEXD_c2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013020; Rad3/Chl1-like.
DR InterPro; IPR030845; RTEL1.
DR PANTHER; PTHR11472; PTHR11472; 1.
DR Pfam; PF06733; DEAD_2; 1.
DR Pfam; PF13307; Helicase_C_2; 1.
DR SMART; SM00488; DEXDc2; 1.
DR SMART; SM00491; HELICc2; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00604; rad3; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51193; HELICASE_ATP_BIND_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase;
KW Hydrolase; Iron; Iron-sulfur; Metal-binding; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..994
FT /note="Regulator of telomere elongation helicase 1 homolog"
FT /id="PRO_0000370616"
FT DOMAIN 15..300
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03065"
FT REGION 876..895
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 251..254
FT /note="DEAH box"
FT BINDING 50..57
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03065"
FT BINDING 142
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03065"
FT BINDING 160
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03065"
FT BINDING 169
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03065"
FT BINDING 208
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03065"
SQ SEQUENCE 994 AA; 112945 MW; 244F62A5E973B7C2 CRC64;
MPPKASNSTE VWINPKLSVK FPFEPYECQR IFMKNVVDVL DRKLDAALES PTGTGKTLSL
LCSTLAWVQR QKETKPLDFA TWQTSGAGGA EKTDEKLKSA YVPTIFYASR THSQLEQVVH
ELNRTEYKWV KTTILGSREH FCINQKVKKI KESNRQAHVC RGLVSKRACH YYNKFDACTT
DKMTEFLDKG EAMDIEDFVK LGTQNSLCPY FMSRQRSETA ELILLPYNYI IDPKMRRRYK
LDLKNSIVIF DEAHNLESIC ESNASAELTS TSIALCIEEL KKVLALLVDE EETARSEADA
ETGAFGSAKI DLTKKLIENL RTEDLMTVLE KIFSLEEEMD KLFGSSQLKS VPPLSGKASD
GEILLETLAK AGFDANSVER LVDVLRDAIS YLLSKNEEVA LTEKGDGMEK VADFLLSIYS
THAQDVAAAV GEETVKLVDR VDPKTVARNC KLYIQKDKDN EKLTIKYFCF QASISMRMLK
MRGVRNVLLA SGTLSPIQAF TYNMGLNFGA ILENEHALKQ VPVLTSIVTR GKRGGLAGSF
QNRKNLDYVT GVAEALLRVM EVIPQGILIF FSSYSQMDEL VATWKTTKWS SNSNESFWEK
MEKTKRVVVE PRAKEELAAI RLRYTQGVSE QHGAALLAVC RGKVSEGIDF CDAESRAVII
IGIPYPPIHD ERVVLKKMYL DDLMGRKDTK SERQSSQDWY QMEAFRAVNQ AIGRVLRHKD
DFGTVVLMDT RYASAKPEMF PKWLRNTISR SDTDGCALKT SRFFKERGHL IENSKTEYIK
KQAKQCKSFR QVKQTAASDS KDDIIEITLE DMFSPANMKL EKKEITKLRP PQLIPSTSSV
FSLPTNEDEL KIKKWEQEND IQCLSSIPLE SNKRKFKIET PGPSTSTLTQ KSEPPKKKKI
LLLTRNTLPD EYQKAIEIPT SELLKDMSDD NKKQFATTLR SYKAESIRWD EVFQRFRPIF
VPHKADLFIA CSNVLRSEDK MKYLKKALES KIHT