RTF1_CAEEL
ID RTF1_CAEEL Reviewed; 613 AA.
AC G5EBY0;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=RNA polymerase-associated protein RTF1 homolog {ECO:0000250|UniProtKB:P53064};
GN Name=rtfo-1 {ECO:0000312|WormBase:F25B3.6};
GN ORFNames=F25B3.6 {ECO:0000312|WormBase:F25B3.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:CAA94759.1};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=24721716; DOI=10.1016/j.ydbio.2014.04.002;
RA Kubota Y., Tsuyama K., Takabayashi Y., Haruta N., Maruyama R., Iida N.,
RA Sugimoto A.;
RT "The PAF1 complex is involved in embryonic epidermal morphogenesis in
RT Caenorhabditis elegans.";
RL Dev. Biol. 391:43-53(2014).
CC -!- FUNCTION: Component of the PAF1 complex which is a multifunctional
CC complex involved in transcription initiation via genetic interactions
CC with TATA-binding proteins, elongation and transcription-coupled
CC histone modification. {ECO:0000250|UniProtKB:P53064}.
CC -!- SUBUNIT: Component of the PAF1 complex which consists of at least cdc-
CC 73, ctr-9, leo-1, pafo-1 and rtfo-1. {ECO:0000250|UniProtKB:P53064}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24721716}.
CC Note=Localization is independent of the other PAF1 complex components.
CC {ECO:0000269|PubMed:24721716}.
CC -!- DEVELOPMENTAL STAGE: Expressed in somatic cells, but not in germ cells,
CC after the 30 cell stage. {ECO:0000269|PubMed:24721716}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knock-down is mostly embryonic
CC lethal. Embryogenesis proceeds more slowly, with embryos displaying
CC defects in the positioning and shape of epidermal cells.
CC {ECO:0000269|PubMed:24721716}.
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DR EMBL; Z70752; CAA94759.1; -; Genomic_DNA.
DR EMBL; Z81101; CAA94759.1; JOINED; Genomic_DNA.
DR PIR; T21325; T21325.
DR RefSeq; NP_505473.1; NM_073072.4.
DR AlphaFoldDB; G5EBY0; -.
DR SMR; G5EBY0; -.
DR ComplexPortal; CPX-966; PAF1 complex.
DR IntAct; G5EBY0; 1.
DR STRING; 6239.F25B3.6; -.
DR EPD; G5EBY0; -.
DR PaxDb; G5EBY0; -.
DR PeptideAtlas; G5EBY0; -.
DR PRIDE; G5EBY0; -.
DR EnsemblMetazoa; F25B3.6.1; F25B3.6.1; WBGene00009103.
DR GeneID; 179345; -.
DR KEGG; cel:CELE_F25B3.6; -.
DR CTD; 179345; -.
DR WormBase; F25B3.6; CE09619; WBGene00009103; rtfo-1.
DR eggNOG; KOG2402; Eukaryota.
DR GeneTree; ENSGT00940000168478; -.
DR HOGENOM; CLU_018644_0_1_1; -.
DR InParanoid; G5EBY0; -.
DR OMA; NVDATDC; -.
DR OrthoDB; 1056075at2759; -.
DR PhylomeDB; G5EBY0; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR PRO; PR:G5EBY0; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00009103; Expressed in embryo and 4 other tissues.
DR GO; GO:0016593; C:Cdc73/Paf1 complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:1990269; F:RNA polymerase II C-terminal domain phosphoserine binding; IBA:GO_Central.
DR GO; GO:0016571; P:histone methylation; IC:ComplexPortal.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR Gene3D; 3.90.70.200; -; 1.
DR InterPro; IPR004343; Plus-3_dom.
DR InterPro; IPR036128; Plus3-like_sf.
DR Pfam; PF03126; Plus-3; 1.
DR SMART; SM00719; Plus3; 1.
DR SUPFAM; SSF159042; SSF159042; 1.
DR PROSITE; PS51360; PLUS3; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..613
FT /note="RNA polymerase-associated protein RTF1 homolog"
FT /id="PRO_0000431508"
FT DOMAIN 252..383
FT /note="Plus3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00693"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 121..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 485..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..613
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 175..209
FT /evidence="ECO:0000255"
FT COILED 425..462
FT /evidence="ECO:0000255"
FT COMPBIAS 8..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..145
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 155..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..546
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 568..589
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 599..613
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 613 AA; 67803 MW; 198487053A5F78BB CRC64;
MSSSESASSD EETKRRAPAT SDSDSDSDAG PKPGKPLSTD SSASDSDAEK PQAKPAKKKT
LTKRKRRATG SSDDDQVDDD LFADKEDKAR WKKLTELEKE QEIFERMEAR ENAIAREEIA
QQLAKKAKKS SEKGVKTEKR RKMNSGGSDA GSPKRKASSD SDSEMDAAFH RPSDINRKHK
EKNAMDALKN KRKEIEKKNA KNEALSIDAV FGANSGSSSS SSSSESSRSS SSSRESSPER
VSEKDKIVKK DVDGLSELRR ARLSRHKLSL MIHAPFFDST VVGCYVRLGQ GQMSGSGSKY
RIWKIVGVEE SNKVYELEGK KTNKIIKCQN GGSERPFRMQ FVSNADFEQI EFDEWLLACK
RHGNLPTVDI MDKKKQDIEK AINHKYSDKE VDLMIKEKSK YQTVPRNFAM TKANWSKQKE
LAQQRGDIRE AEQIQTKIDE IERQADELEK ERSKSISAIA FINHRNRSKI KDQVLSGQLK
IEENSQDDPF TRKKGGMRVV SGSKSRLDGT LSASSSTTNL SDGGKDKSSS LAKPTQPPPS
TQIKKKTDIS SLHDFDLDID LGKLKDFSTP ESSGNKRPSI SSSKGVSLSD YRMRRSGGGD
AGSSTSAAPS SAV
