RTJK_DROFU
ID RTJK_DROFU Reviewed; 916 AA.
AC P21329;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=RNA-directed DNA polymerase from mobile element jockey;
DE EC=2.7.7.49;
DE AltName: Full=Reverse transcriptase;
GN Name=jockey\pol; Synonyms=pol;
OS Drosophila funebris (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila.
OX NCBI_TaxID=7221;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1911.1;
RX PubMed=1701254; DOI=10.1073/pnas.87.23.9216;
RA Mizrokhi L.J., Mazo A.M.;
RT "Evidence for horizontal transmission of the mobile element jockey between
RT distant Drosophila species.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:9216-9220(1990).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00405};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC -!- ACTIVITY REGULATION: Inactivated by sulphydryl reagent.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Temperature dependence:
CC Optimum temperature is 26 degrees Celsius.;
CC -!- MISCELLANEOUS: Prefers poly(rC) and poly(rA) as template and activated
CC DNA is not effective.
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DR EMBL; M38437; AAA28649.1; -; Genomic_DNA.
DR PIR; A38418; A38418.
DR AlphaFoldDB; P21329; -.
DR SMR; P21329; -.
DR FlyBase; FBgn0019258; Dfun\jockey\pol.
DR GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.60.10.10; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR InterPro; IPR000477; RT_dom.
DR Pfam; PF14529; Exo_endo_phos_2; 1.
DR Pfam; PF00078; RVT_1; 1.
DR SUPFAM; SSF56219; SSF56219; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 1: Evidence at protein level;
KW Nucleotidyltransferase; RNA-directed DNA polymerase; Transferase;
KW Transposable element.
FT CHAIN 1..916
FT /note="RNA-directed DNA polymerase from mobile element
FT jockey"
FT /id="PRO_0000097513"
FT DOMAIN 483..757
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT REGION 890..916
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 916 AA; 103470 MW; A3BDBEE261B61462 CRC64;
MTQPTIKIGL WMHRGLTRGS EELRLFLSVH NIDVMLVTES HMREGQRIFL PGYSTYHVFH
PSGNSRGGAS VIVRSSISHS PQPPISTNDR QIAYIQLQTA EGPVVLAAVY LPPRERWIRA
EFESLFAVLG NKFIAGGDYN AKHAWWGNSR ACARGKVLQE VVANGQYQIL ATGEPTFYSY
NPLVSPSALD FFVVNGYDMR RLNVQTLHEL SSDHTPLLAD LHAMPINKPP RSCLLARGAD
IERFKAYLTQ HIDLSVGIQG TDDIDNAIDS FMDILKRAAI RSAPSHQQNV ESSRQLQLPP
IVASLIRLKR KVRREYARTG DARIQQIHSR LANRLHKVLN RRKQSQIDNL LENLDTDGST
NFSLWRITKR YKTQATPNSA IRNPAGGWCR TSREKTEVFA NHLEQRFKAL AFAPESHSLM
VAESLQTPFQ MALPADPVTL EEVKELVSKL KPKKAPGEDL LDNRTIRLLP DQALLYLVLI
FNSILRVGYF PKARPTASII MILKPGKQPL DVDSYRPTSL LPSLGKMLER LILNRILTSE
EVTRAIPKFQ FGFRLQHGTP EQLHRVVNFA LEALEKKEYA GSCFLDIQQA FDRVWHPGLL
YKAKSLLSPQ LFQLIKSFWE GRKFSVTADG CRSSVKFIEA GVPQGSVLGP TLYSIFTADM
PNQNAVTGLA EGEVLIATYA DDIAVLTKST CIVEATDALQ EYLDAFQEWA VKWNVSINAG
KCANVTFTNA IRDCPGVTIN GSLLSHTHEY KYLGVILDRS LTFRRHITSL QHSFRTRITK
MNWLLAARNK LSLDNKVKIY KCIVAPGLFY AIQVYGIAAR THLNKIRVLQ AKMLRKISGA
PWYMRTRDIE CDLKVPKIGD KIREVAKKYH ERLDSHPNSL ARRLGVAAQR SASPRSRVRR
RLKRHHPQDL LDRALT
