ID   RTJK_DROME              Reviewed;         916 AA.
AC   P21328;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=RNA-directed DNA polymerase from mobile element jockey;
DE            EC=2.7.7.49;
DE   AltName: Full=Reverse transcriptase;
GN   Name=pol;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2463954; DOI=10.1016/0378-1119(88)90197-7;
RA   Priimaegi A.F., Mizrokhi L.J., Ilyin Y.V.;
RT   "The Drosophila mobile element jockey belongs to LINEs and contains coding
RT   sequences homologous to some retroviral proteins.";
RL   Gene 70:253-262(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-191.
RX   PubMed=3040362;
RA   Mizrokhi L.J., Priimaegi A.F., Ilyin Y.V.;
RT   "Drosophila mobile element jockey is a retroposon and encodes the GAG-
RT   specific protein sequence characteristic for retroviruses.";
RL   Dokl. Akad. Nauk SSSR 294:1235-1239(1987).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=1714378; DOI=10.1002/j.1460-2075.1991.tb07788.x;
RA   Ivanov V.A., Melnikov A.A., Siunov A.V., Fodor I.I., Ilyin Y.V.;
RT   "Authentic reverse transcriptase is coded by jockey, a mobile Drosophila
RT   element related to mammalian LINEs.";
RL   EMBO J. 10:2489-2495(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC   -!- ACTIVITY REGULATION: Inactivated by sulphydryl reagent.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Optimum temperature is 26 degrees Celsius.;
CC   -!- MISCELLANEOUS: Prefers poly(rC) and poly(rA) as template and activated
CC       DNA is not effective.
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DR   EMBL; M22874; AAA28675.1; -; Genomic_DNA.
DR   EMBL; M38643; AAA28940.1; -; Genomic_DNA.
DR   PIR; JT0396; JT0396.
DR   AlphaFoldDB; P21328; -.
DR   SMR; P21328; -.
DR   FlyBase; FBgn0015952; jockey\pol.
DR   PRO; PR:P21328; -.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR000477; RT_dom.
DR   Pfam; PF14529; Exo_endo_phos_2; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   1: Evidence at protein level;
KW   Nucleotidyltransferase; RNA-directed DNA polymerase; Transferase;
KW   Transposable element.
FT   CHAIN           1..916
FT                   /note="RNA-directed DNA polymerase from mobile element
FT                   jockey"
FT                   /id="PRO_0000097514"
FT   DOMAIN          483..757
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   CONFLICT        185..191
FT                   /note="TPSALDF -> LKFIRII (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   916 AA;  103431 MW;  2105551F2E064CD1 CRC64;
     MTQPTLKIGL WNARGLTRGS EELRIFLSDH DIDVMLTTET HMRVGQRIYL PGYLMYHAHH
     PSGNSRGGSA VIIKSRLCHS PLTPISTNDR QIARVHLQTS VGTVTVAAVY LPPAERWIVD
     DFKSMFAALG NKFIAGGDYN AKHAWWGNPR SCPRGKMLQE VIAHGQYQVL ATGEPTFYSY
     NPLLTPSALD FFITCGYGMG RLDVQTLQEL SSDHLPILAV LHATPLKKPQ RVRLLAHNAD
     INIFKTHLEQ LSEVNMQILE AVDIDNATSL FMSKLSEAAQ LAAPRNRHEV EAFRPLQLPS
     SILALLRLKR RVRKEYARTG DPRMQQIHSR LANCLHKALA RRKQAQIDTF LDNLGADAST
     NYSLWRITKR FKAQPTPKSA IKNPSGGWCR TSLEKTEVFA NNLEQRFTPY NYAPESLCRQ
     VEEYLESPFQ MSLPLSAVTL EEVKNLIAKL PLKKAPGEDL LDNRTIRLLP DQALQFLALI
     FNSVLDVGYF PKAWKSASII MIHKTGKTPT DVDSYRPTSL LPSLGKIMER LILNRLLTCK
     DVTKAIPKFQ FGFRLQHGTP EQLHRVVNFA LEAMENKEYA VGAFLDIQQA FDRVWHPGLL
     YKAKRLFPPQ LYLVVKSFLE ERTFHVSVDG YKSSIKPIAA GVPQGSVLGP TLYSVFASDM
     PTHTPVTEVD EEDVLIATYA DDTAVLTKSK SILAATSGLQ EYLDAFQQWA ENWNVRINAE
     KCANVTFANR TGSCPGVSLN GRLIRHHQAY KYLGITLDRK LTFSRHITNI QQAFRTKVAR
     MSWLIAPRNK LSLGCKVNIY KSILAPCLFY GLQVYGIAAK SHLNKIRILQ AKTLRRISGA
     PWYMRTRDIE RDLKVPKLGD KLQNIAQKYM ERLNVHPNSL ARKLGTAAVV NADPRTRVKR
     RLKRHHPHDL PNLVLT