RTKN2_MOUSE
ID RTKN2_MOUSE Reviewed; 604 AA.
AC Q14B46; Q8BFP3; Q8BFP8; Q8BFQ0;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Rhotekin-2;
DE AltName: Full=Pleckstrin homology domain-containing family K member 1;
DE Short=PH domain-containing family K member 1;
GN Name=Rtkn2; Synonyms=Plekhk1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-439 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo, Kidney, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play an important role in lymphopoiesis. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q14B46-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q14B46-2; Sequence=VSP_029140;
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DR EMBL; AK030986; BAC27204.1; -; mRNA.
DR EMBL; AK045134; BAC32237.1; -; mRNA.
DR EMBL; AK052662; BAC35087.1; -; mRNA.
DR EMBL; BC116341; AAI16342.1; -; mRNA.
DR EMBL; BC116342; AAI16343.1; -; mRNA.
DR CCDS; CCDS88017.1; -. [Q14B46-2]
DR CCDS; CCDS88018.1; -. [Q14B46-1]
DR RefSeq; XP_006513353.1; XM_006513290.2.
DR RefSeq; XP_006513354.1; XM_006513291.1.
DR AlphaFoldDB; Q14B46; -.
DR SMR; Q14B46; -.
DR BioGRID; 228447; 3.
DR STRING; 10090.ENSMUSP00000112946; -.
DR iPTMnet; Q14B46; -.
DR PhosphoSitePlus; Q14B46; -.
DR jPOST; Q14B46; -.
DR MaxQB; Q14B46; -.
DR PaxDb; Q14B46; -.
DR PRIDE; Q14B46; -.
DR ProteomicsDB; 257051; -. [Q14B46-1]
DR ProteomicsDB; 257052; -. [Q14B46-2]
DR Antibodypedia; 28261; 139 antibodies from 24 providers.
DR Ensembl; ENSMUST00000068994; ENSMUSP00000070717; ENSMUSG00000037846. [Q14B46-1]
DR Ensembl; ENSMUST00000117086; ENSMUSP00000112419; ENSMUSG00000037846. [Q14B46-2]
DR Ensembl; ENSMUST00000118160; ENSMUSP00000112946; ENSMUSG00000037846. [Q14B46-1]
DR Ensembl; ENSMUST00000147556; ENSMUSP00000116166; ENSMUSG00000037846. [Q14B46-2]
DR UCSC; uc011xfq.1; mouse. [Q14B46-1]
DR MGI; MGI:2158417; Rtkn2.
DR VEuPathDB; HostDB:ENSMUSG00000037846; -.
DR eggNOG; ENOG502QRWR; Eukaryota.
DR GeneTree; ENSGT00940000157470; -.
DR HOGENOM; CLU_025066_1_0_1; -.
DR InParanoid; Q14B46; -.
DR OMA; NWSTLFD; -.
DR OrthoDB; 497196at2759; -.
DR PhylomeDB; Q14B46; -.
DR TreeFam; TF331476; -.
DR BioGRID-ORCS; 170799; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q14B46; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q14B46; protein.
DR Bgee; ENSMUSG00000037846; Expressed in right lung lobe and 114 other tissues.
DR ExpressionAtlas; Q14B46; baseline and differential.
DR Genevisible; Q14B46; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0030097; P:hemopoiesis; IDA:MGI.
DR GO; GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; ISS:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:MGI.
DR GO; GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
DR GO; GO:1901224; P:positive regulation of NIK/NF-kappaB signaling; ISS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR012966; AHD.
DR InterPro; IPR011072; HR1_rho-bd.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR030469; RTKN2.
DR PANTHER; PTHR21538:SF21; PTHR21538:SF21; 1.
DR Pfam; PF08174; Anillin; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00742; Hr1; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS51860; REM_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Reference proteome.
FT CHAIN 1..604
FT /note="Rhotekin-2"
FT /id="PRO_0000309327"
FT DOMAIN 1..74
FT /note="REM-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01207"
FT DOMAIN 281..387
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 481..590
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 53..79
FT /evidence="ECO:0000255"
FT COMPBIAS 510..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 80..82
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029140"
FT CONFLICT 408
FT /note="R -> L (in Ref. 1; BAC27204)"
FT /evidence="ECO:0000305"
FT CONFLICT 449
FT /note="R -> G (in Ref. 2; AAI16342/AAI16343)"
FT /evidence="ECO:0000305"
FT CONFLICT 484
FT /note="I -> T (in Ref. 2; AAI16342/AAI16343)"
FT /evidence="ECO:0000305"
FT CONFLICT 515
FT /note="R -> S (in Ref. 2; AAI16342/AAI16343)"
FT /evidence="ECO:0000305"
FT CONFLICT 533..534
FT /note="SS -> PL (in Ref. 2; AAI16342/AAI16343)"
FT /evidence="ECO:0000305"
FT CONFLICT 541..542
FT /note="SP -> LL (in Ref. 2; AAI16342/AAI16343)"
FT /evidence="ECO:0000305"
FT CONFLICT 560
FT /note="A -> G (in Ref. 1; BAC27204)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 604 AA; 66962 MW; 9DC76A294B1DE49B CRC64;
MEGQLLRGLA AQQDCSIREK IDLEIRMREG IWKLLSLSTK KDQVLHAVKN LMVCSARIQA
YTAELQKSKE EIANQTGARL VLDSSSENKE GESCRGKIAL SDIRIPLMWK DSDHFSNKEC
TQRFAIFCLF RMGAQVFDTD MVIVDQTVTD ICFENVTIFN EAGPDFQIKI EVYSCSAEES
SLTNTPRKLA KKLKTSISKA TGRKISAALQ EESPEACLLA GSVAGAKYHL LAHTTLTLEN
AGDCFKTHNL SVHGDEECSF WLPLYGNVCC RLVAQPACMA ADAFAGFLNE QQTGKGLVGW
RRLYCALRGG KLRCFYGPEE IEAKVEPALV VPIDKETRIQ AVEKDSKKMH CFSVLSTAAG
RAVSHIFAAD SLADFQEWMG AFRQHFFDLS QWKHCCEELM RIEIMSPRKP PLFLAKEATS
VYYDMSIDSP VKLESVTDII QKKIGETNRQ FLIGRDDQSA APPWAAVFDG NHEMVIEKKV
LSPIGEPAPD GKRKKRRAPL PPTDQPPFCI KTQGRANQSK DSATQAGVSG ASSSPSDPRL
SPPTHHLQKP VAAPRKLLPA RKNSSADIGH TDTKTSLDAK PVPVPRQKSI RDILDPRSWL
QAQV
