RTL2_ORYSJ
ID RTL2_ORYSJ Reviewed; 317 AA.
AC Q6ATG6; A0A0P0WJY4;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Ribonuclease 3-like protein 2;
DE EC=3.1.26.-;
DE AltName: Full=Ribonuclease III-like protein 2;
DE Short=RNase III-like protein 2;
GN OrderedLocusNames=Os05g0271300, LOC_Os05g18850;
GN ORFNames=OJ1653_D06.9, OSJNBb0061M13.3;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18826656; DOI=10.1186/1471-2164-9-451;
RA Kapoor M., Arora R., Lama T., Nijhawan A., Khurana J.P., Tyagi A.K.,
RA Kapoor S.;
RT "Genome-wide identification, organization and phylogenetic analysis of
RT dicer-like, argonaute and RNA-dependent RNA polymerase gene families and
RT their expression analysis during reproductive development and stress in
RT rice.";
RL BMC Genomics 9:451-451(2008).
CC -!- FUNCTION: Cleaves double-stranded RNA (dsRNA). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT85223.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAV67838.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF16975.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC115634; AAV67838.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC137002; AAT85223.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008211; BAF16975.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; BAS93091.1; -; Genomic_DNA.
DR AlphaFoldDB; Q6ATG6; -.
DR SMR; Q6ATG6; -.
DR STRING; 4530.OS05T0271300-00; -.
DR PaxDb; Q6ATG6; -.
DR PRIDE; Q6ATG6; -.
DR EnsemblPlants; Os05t0271300-00; Os05t0271300-00; Os05g0271300.
DR Gramene; Os05t0271300-00; Os05t0271300-00; Os05g0271300.
DR eggNOG; KOG0701; Eukaryota.
DR HOGENOM; CLU_000907_5_3_1; -.
DR InParanoid; Q6ATG6; -.
DR OMA; DIKHWKN; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q6ATG6; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR CDD; cd00593; RIBOc; 1.
DR Gene3D; 1.10.1520.10; -; 1.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; SSF69065; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 2: Evidence at transcript level;
KW Endonuclease; Hydrolase; Magnesium; Manganese; Metal-binding; Nuclease;
KW Reference proteome; RNA-binding.
FT CHAIN 1..317
FT /note="Ribonuclease 3-like protein 2"
FT /id="PRO_0000378423"
FT DOMAIN 37..185
FT /note="RNase III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00177"
FT DOMAIN 211..274
FT /note="DRBM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 74
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 171
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT SITE 167
FT /note="Important for activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 317 AA; 34954 MW; 356B83BDFEF19651 CRC64;
MAPPPAMKPA SRKRGPPAPD PVELPPPGFV ADRAEAAARV ERLLRYQFRD GRLLEEALTH
QSFADDAVSY QRLEFVGDSA LGLAFSNFLY LTNPTLGPGP LSTLRAANIS TEKLARVAVR
HDLYPLLRRN CPRLDLLVGQ FIETVKQEPE DDLSTVPYGG SVVKAPKVLA DIVEAIAAAV
YVDCKFDLEK LWKVTRWLFE PIITAETIDE QPVTMLHELC QKHGKMAQFK TWQKGGMTVV
NVFVAGELVG IGSSEQKVIA KLNAARDATR KLAGAKKQVL TTGVGNGLGD EIGELRECKQ
KLNEQCSRQN WPKPIFK
