ID   RTL3_ARATH              Reviewed;         957 AA.
AC   Q9FKF0;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Ribonuclease 3-like protein 3;
DE            EC=3.1.26.-;
DE   AltName: Full=Ribonuclease III-like protein 3;
DE            Short=RNase III-like protein 3;
DE   AltName: Full=Ribonuclease three-like protein 3;
GN   Name=RTL3; OrderedLocusNames=At5g45150; ORFNames=K18C1.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Ribonuclease that cleaves double-stranded RNA (dsRNA).
CC       {ECO:0000250}.
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DR   EMBL; AB012240; BAB11388.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95210.1; -; Genomic_DNA.
DR   RefSeq; NP_199328.1; NM_123883.2.
DR   AlphaFoldDB; Q9FKF0; -.
DR   SMR; Q9FKF0; -.
DR   STRING; 3702.AT5G45150.1; -.
DR   iPTMnet; Q9FKF0; -.
DR   PaxDb; Q9FKF0; -.
DR   PRIDE; Q9FKF0; -.
DR   ProteomicsDB; 226573; -.
DR   EnsemblPlants; AT5G45150.1; AT5G45150.1; AT5G45150.
DR   GeneID; 834551; -.
DR   Gramene; AT5G45150.1; AT5G45150.1; AT5G45150.
DR   KEGG; ath:AT5G45150; -.
DR   Araport; AT5G45150; -.
DR   TAIR; locus:2153333; AT5G45150.
DR   eggNOG; KOG0701; Eukaryota.
DR   HOGENOM; CLU_323998_0_0_1; -.
DR   InParanoid; Q9FKF0; -.
DR   OrthoDB; 935825at2759; -.
DR   PhylomeDB; Q9FKF0; -.
DR   PRO; PR:Q9FKF0; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FKF0; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR   CDD; cd00593; RIBOc; 2.
DR   Gene3D; 1.10.1520.10; -; 2.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00035; dsrm; 1.
DR   Pfam; PF00636; Ribonuclease_3; 2.
DR   SMART; SM00358; DSRM; 3.
DR   SMART; SM00535; RIBOc; 2.
DR   SUPFAM; SSF69065; SSF69065; 2.
DR   PROSITE; PS50137; DS_RBD; 3.
DR   PROSITE; PS50142; RNASE_3_2; 2.
PE   2: Evidence at transcript level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Repeat; RNA-binding.
FT   CHAIN           1..957
FT                   /note="Ribonuclease 3-like protein 3"
FT                   /id="PRO_0000404664"
FT   DOMAIN          4..142
FT                   /note="RNase III 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00177"
FT   DOMAIN          307..382
FT                   /note="DRBM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          415..551
FT                   /note="RNase III 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00177"
FT   DOMAIN          566..645
FT                   /note="DRBM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          837..912
FT                   /note="DRBM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
SQ   SEQUENCE   957 AA;  108300 MW;  3D6FF2E7D117EEFB CRC64;
     MNSVEAVEKI LNYSFVNKTL LKEAITQKSP LFDRLEFFGD SILEVAFTNY ICHTYPNLKV
     KELRDLRTAN VSNEKFARIA VNHNLHHFLL LQNPSLFKKV KNFAEAVRKE DDPVPYGGLV
     KAPKILADTL ESIAATVFID VNYDVKRLWE IFRSLLEPIY TPDDLLLQPK LPFLTLFRLA
     DKHGSESTSG IRKMTTSTKI LLRNKDCLDV DLEDVKGKSF EICSTELFSL STVSENSLTD
     EMSQEEVVID EDSPNVEPED VKGKLFEICY TRKLQIQTGS SGNPLTYEMT TKQMVVDKDS
     LHVEPVNGRG ELIEICTKNK WPRPIFSVQE EKGPKNEPKF VCSVKIEIPN IEGTFHMKGD
     IKSKKKQAEN SLAYHMIRAL ESSLVSLVIS NLQKPKSLDE KNNPLLFSSE MDSSVEAVEK
     ILNYSFVNKT LLKELLTHNN SPLFQGLMFV GESALSLAFT KHLYLTYPML EPKDLSVLRD
     ANTCHDKYAC VAVKKGIYQS FIGSVPKPEK MTTDFIELMG KEDDPYRVVK APKILVNLLA
     GVAGAVYIDV KYNVQRLLEI FRVLLEPIYT LDDLRMQLKQ PFLMLFRLAD KHGKQIDFRY
     SKDGGSRKNI AQVYMDDMFI ASGCAKRIDT AKLLAAEEAI QKLSECMPIE KIIHQDNLDG
     EVIQTGSSSL LTAFENPLTE EMTQEQMVID EDSLDVEWKL FETEELQIQT GSSSMSTVSE
     NPLPCEITPT KMVIGEVSPH VELEDVKGKS FEISSTETSS LPIAFENPLT NELTQEQMVI
     DENSPYVEPV DTKGKLFESC SVESSSLPTT SENPSTYEMT TKQMVVDKDS PHVEPEDEKG
     KLFEICAKNK WPNPIFSVEE ERGQQNEQKI VCSVKIEIPN IEGTFHIKGD AKPTKKEAEN
     SSADHMIRAL ESSVMSLVIT NLQMHENLDG KKKNLQMKES LNENKTLLHS TKRRRRL