RTN1_MOUSE
ID RTN1_MOUSE Reviewed; 780 AA.
AC Q8K0T0; Q8K4S4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Reticulon-1;
DE AltName: Full=Neuroendocrine-specific protein;
GN Name=Rtn1; Synonyms=Nsp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=ICR; TISSUE=Brain;
RX PubMed=12036513; DOI=10.1016/s0165-3806(02)00304-8;
RA Hirata T., Nomura T., Takagi Y., Sato Y., Tomioka N., Fujisawa H.,
RA Osumi N.;
RT "Mosaic development of the olfactory cortex with Pax6-dependent and
RT -independent components.";
RL Brain Res. Dev. Brain Res. 136:17-26(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 181-190; 238-248; 647-654 AND 759-767, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [5]
RP IDENTIFICATION.
RX PubMed=12832288; DOI=10.1096/fj.02-1166hyp;
RA Oertle T., Klinger M., Stuermer C.A.O., Schwab M.E.;
RT "A reticular rhapsody: phylogenic evolution and nomenclature of the
RT RTN/Nogo gene family.";
RL FASEB J. 17:1238-1247(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350; SER-352 AND SER-487, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Inhibits amyloid precursor protein processing, probably by
CC blocking BACE1 activity. {ECO:0000250|UniProtKB:Q16799}.
CC -!- SUBUNIT: Interacts with NDRG1. Interacts with BACE1. Interacts with
CC TMEM33. {ECO:0000250|UniProtKB:Q16799}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:12036513}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q16799};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: At 12.5 dpc-14.5 dpc, strongly expressed in radial
CC glial fibers, which are a scaffold for migrating neurons (at protein
CC level). {ECO:0000269|PubMed:12036513}.
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DR EMBL; AB074899; BAB96551.1; -; mRNA.
DR EMBL; BC030455; AAH30455.1; -; mRNA.
DR EMBL; BC053926; AAH53926.1; -; mRNA.
DR EMBL; BC058579; AAH58579.1; -; mRNA.
DR EMBL; BK001694; DAA01939.1; -; mRNA.
DR CCDS; CCDS25968.1; -.
DR RefSeq; NP_001273377.1; NM_001286448.1.
DR RefSeq; NP_703187.2; NM_153457.7.
DR AlphaFoldDB; Q8K0T0; -.
DR SMR; Q8K0T0; -.
DR BioGRID; 222271; 12.
DR CORUM; Q8K0T0; -.
DR IntAct; Q8K0T0; 3.
DR STRING; 10090.ENSMUSP00000077594; -.
DR iPTMnet; Q8K0T0; -.
DR PhosphoSitePlus; Q8K0T0; -.
DR SwissPalm; Q8K0T0; -.
DR jPOST; Q8K0T0; -.
DR MaxQB; Q8K0T0; -.
DR PaxDb; Q8K0T0; -.
DR PRIDE; Q8K0T0; -.
DR ProteomicsDB; 262724; -.
DR Antibodypedia; 3447; 509 antibodies from 26 providers.
DR DNASU; 104001; -.
DR Ensembl; ENSMUST00000078505; ENSMUSP00000077594; ENSMUSG00000021087.
DR GeneID; 104001; -.
DR KEGG; mmu:104001; -.
DR UCSC; uc007nvk.2; mouse.
DR CTD; 6252; -.
DR MGI; MGI:1933947; Rtn1.
DR VEuPathDB; HostDB:ENSMUSG00000021087; -.
DR eggNOG; KOG1792; Eukaryota.
DR GeneTree; ENSGT00940000155077; -.
DR HOGENOM; CLU_018293_0_0_1; -.
DR InParanoid; Q8K0T0; -.
DR OrthoDB; 244299at2759; -.
DR PhylomeDB; Q8K0T0; -.
DR TreeFam; TF105431; -.
DR BioGRID-ORCS; 104001; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Rtn1; mouse.
DR PRO; PR:Q8K0T0; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8K0T0; protein.
DR Bgee; ENSMUSG00000021087; Expressed in dentate gyrus of hippocampal formation granule cell and 187 other tissues.
DR ExpressionAtlas; Q8K0T0; baseline and differential.
DR Genevisible; Q8K0T0; MM.
DR GO; GO:0030425; C:dendrite; IDA:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:MGI.
DR GO; GO:0000139; C:Golgi membrane; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043025; C:neuronal cell body; IDA:MGI.
DR GO; GO:0005790; C:smooth endoplasmic reticulum; ISO:MGI.
DR GO; GO:1902430; P:negative regulation of amyloid-beta formation; ISS:UniProtKB.
DR InterPro; IPR003388; Reticulon.
DR Pfam; PF02453; Reticulon; 1.
DR PROSITE; PS50845; RETICULON; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; Golgi apparatus;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..780
FT /note="Reticulon-1"
FT /id="PRO_0000168159"
FT TRANSMEM 607..627
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 709..729
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 593..780
FT /note="Reticulon"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00170"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 128..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 390..411
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..515
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 546..573
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 13
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64548"
FT MOD_RES 70
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64548"
FT MOD_RES 327
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64548"
FT MOD_RES 350
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 352
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 487
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 71
FT /note="P -> S (in Ref. 1; BAB96551)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="D -> G (in Ref. 1; BAB96551)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 780 AA; 83572 MW; 29B47A58FC2F2027 CRC64;
MAAPPDLQDE PLSLGSPGSQ WFGGRGDGED EATAVMGARP AQQDGEPAWG SGAGAGVTSS
RELCSGPARS PPVAMETAST GMAAVPDALD HSPSSTLKDG EGACYTSLIS DVCYPPREDS
AYFTGILQKE NGHITTSESP EEPETPGPSL PEVPGMEPQG LLSSDSGIEM TPAESTEVNK
ILADPLDQMK AEAYKYIDIT RPQEAKGQEE QHPGLEDKDL DFKDKDTEVS TKAEGVRAPN
QPAPVEGKLI KDHLFEESTF APYIDELSDE QHRVSLVTAP VKITLTEIEP PLMTATQETI
PEKQDLCLKP SPDTVPTVTV SEPEDDSPGS VTPPSSGTEP SAAESQGKGS VSEDELIAAI
KEAKGLSYET TESPRPVGQV ADKPKTKTRS GLPTIPSPLD QEASSAESGD SEIELVSEDP
MASEDALPSG YVSFGHVSGP PPSPASPSIQ YSILREEREA ELDSELIIES CDASSASEES
PKREQDSPPM KPGALDAIRE ETGSRATEER APSHQGPVEP DPMLSFAPAA ALQSRPEPSS
GDGASVPEPP RSQQQKPEEE AVSSSQSPTA TEIPGPLGSG LMPPLPFFNK QKAIDLLYWR
DIKQTGIVFG SFLLLLFSLT QFSVVSVVAY LALAALSATI SFRIYKSVLQ AVQKTDEGHP
FKAYLELEIT LSQEQIQKYT DCLQLYVNST LKELRRLFLV QDLVDSLKFA VLMWLLTYVG
ALFNGLTLLL MAVVSMFTLP VVYVKHQAQV DQYLGLVRTH INTVVAKIQA KIPGAKRHAE
