ID   BCRA_ENTFL              Reviewed;         308 AA.
AC   Q5WNX0;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Bacitracin transport ATP-binding protein BcrA {ECO:0000305};
GN   Name=bcrA {ECO:0000303|PubMed:15388429};
OS   Enterococcus faecalis (Streptococcus faecalis).
OG   Plasmid pJM01.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=AR01/DGVS;
RX   PubMed=15388429; DOI=10.1128/aac.48.10.3743-3748.2004;
RA   Manson J.M., Keis S., Smith J.M.B., Cook G.M.;
RT   "Acquired bacitracin resistance in Enterococcus faecalis is mediated by an
RT   ABC transporter and a novel regulatory protein, BcrR.";
RL   Antimicrob. Agents Chemother. 48:3743-3748(2004).
RN   [2]
RP   INDUCTION.
RC   STRAIN=AR01/DGVS;
RX   PubMed=18227063; DOI=10.1074/jbc.m709503200;
RA   Gauntlett J.C., Gebhard S., Keis S., Manson J.M., Pos K.M., Cook G.M.;
RT   "Molecular analysis of BcrR, a membrane-bound bacitracin sensor and DNA-
RT   binding protein from Enterococcus faecalis.";
RL   J. Biol. Chem. 283:8591-8600(2008).
CC   -!- FUNCTION: Essential for high-level bacitracin resistance
CC       (PubMed:15388429). Part of the ABC transporter complex BcrAB. Probably
CC       responsible for energy coupling to the transport system (Probable).
CC       {ECO:0000269|PubMed:15388429, ECO:0000305|PubMed:15388429}.
CC   -!- SUBUNIT: The complex is probably composed of two ATP-binding proteins
CC       (BcrA) and two transmembrane proteins (BcrB). {ECO:0000305}.
CC   -!- INDUCTION: Transcription is activated by the regulatory protein BcrR in
CC       the presence of bacitracin. {ECO:0000269|PubMed:15388429,
CC       ECO:0000269|PubMed:18227063}.
CC   -!- DISRUPTION PHENOTYPE: Disruption mutant is sensitive to bacitracin.
CC       {ECO:0000269|PubMed:15388429}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; AY496968; AAS78451.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5WNX0; -.
DR   SMR; Q5WNX0; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic resistance; ATP-binding; Nucleotide-binding; Plasmid; Transport.
FT   CHAIN           1..308
FT                   /note="Bacitracin transport ATP-binding protein BcrA"
FT                   /id="PRO_0000447366"
FT   DOMAIN          8..236
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   308 AA;  34291 MW;  ED194215F70E1FC6 CRC64;
     MMIMEYVIET ENLTKQYGET TVVNKINLHV PKGKIYGLLG RNGAGKTTAM KMMLQLAFPT
     DGTVRLFGTN YKENIHTLYS KVGSIIETPG FYSNLTGYEN LQILAKLRGG VSKSGVEKAL
     EVVGLHKEKR KVFSDYSLGM KQRLGIAAAI MHEPELLILD EPINGLDPIG ISEIRSFLSK
     LSHENGTTIF ISSHVLSEIE QIADVIGVMH EGHLVEEVNI SELHKRNRKY TEFDVSDGKI
     AAKILESSYH MTDFTVQDGT IRIYDFSQSV GEINREFARN GLLITRINDS EENLEDYFSK
     LIGGGGIA