RTOA_DICDI
ID RTOA_DICDI Reviewed; 367 AA.
AC P54681; Q55AD6; Q86KL5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Protein rtoA;
DE AltName: Full=Ratio-A;
DE Flags: Precursor;
GN Name=rtoA; ORFNames=DDB_G0271916;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX PubMed=8951083; DOI=10.1242/dev.122.11.3677;
RA Wood S.A., Ammann R.R., Brock D.A., Li L., Spann T., Gomer R.H.;
RT "RtoA links initial cell type choice to the cell cycle in Dictyostelium.";
RL Development 122:3677-3685(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=10747962; DOI=10.1074/jbc.m000900200;
RA Brazill D.T., Caprette D.R., Myler H.A., Hatton R.D., Ammann R.R.,
RA Lindsey D.F., Brock D.A., Gomer R.H.;
RT "A protein containing a serine-rich domain with vesicle fusing properties
RT mediates cell cycle-dependent cytosolic pH regulation.";
RL J. Biol. Chem. 275:19231-19240(2000).
RN [5]
RP INDUCTION.
RX PubMed=12771188; DOI=10.1242/jcs.00501;
RA Araki T., Tsujioka M., Abe T., Fukuzawa M., Meima M., Schaap P., Morio T.,
RA Urushihara H., Katoh M., Maeda M., Tanaka Y., Takeuchi I., Williams J.G.;
RT "A STAT-regulated, stress-induced signalling pathway in Dictyostelium.";
RL J. Cell Sci. 116:2907-2915(2003).
RN [6]
RP DISRUPTION PHENOTYPE, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15679845; DOI=10.1111/j.1462-5822.2004.00472.x;
RA Li Z., Solomon J.M., Isberg R.R.;
RT "Dictyostelium discoideum strains lacking the RtoA protein are defective
RT for maturation of the Legionella pneumophila replication vacuole.";
RL Cell. Microbiol. 7:431-442(2005).
RN [7]
RP INDUCTION.
RX PubMed=16469056; DOI=10.1111/j.1462-5822.2005.00633.x;
RA Farbrother P., Wagner C., Na J., Tunggal B., Morio T., Urushihara H.,
RA Tanaka Y., Schleicher M., Steinert M., Eichinger L.;
RT "Dictyostelium transcriptional host cell response upon infection with
RT Legionella.";
RL Cell. Microbiol. 8:438-456(2006).
CC -!- FUNCTION: May have dual functions, one in vegetative cells at
CC starvation involving cell-type choice and a later function involving
CC progression to the tipped mound stage. Involved in vesicle fusion in
CC endo- and exocytosis. Involved in L.pneumophila intracellular growth.
CC {ECO:0000269|PubMed:15679845}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10747962,
CC ECO:0000269|PubMed:15679845}. Note=Mostly found in the cytosol but also
CC found associated with both small organelles or vesicles.
CC -!- DEVELOPMENTAL STAGE: Expressed in vegetative cells. Levels decrease
CC dramatically as development begins, and remain low throughout the
CC aggregation and loose mound stages. The levels become more abundant
CC again at the tight-mound stage and remain high throughout the rest of
CC development and fruiting body formation.
CC -!- INDUCTION: Rapidly up-regulated by hyperosmotic stress, which is
CC dependent on dstC. Strongly inducible by 8-bromo-cGMP and to a lesser
CC extent by 8-bromo-cAMP. Not induced by DIF (differentiation-inducing
CC factor, a chlorinated hexaphenone). Up-regulated following Legionella
CC pneumophilia infection. {ECO:0000269|PubMed:12771188,
CC ECO:0000269|PubMed:16469056}.
CC -!- DISRUPTION PHENOTYPE: Aberrant ratio of prespore to prestalk cells.
CC Cells stall and only 10% of the aggregates form fruiting bodies. The
CC fruiting bodies that do form have abnormal shapes, with enlarged stalks
CC and aberrantly small mass of spores. Cells also show a decreased
CC exocytosis rate, decreased pH of endocytic vesicles, lack of
CC morphologically distinguishable post-lysosomal compartments as well as
CC perturbation of the cell cycle-regulated cytosolic pH. Cells infected
CC with Legionella pneumophilia are defective for maturation of the
CC L.pneumophilia replication vacuole. {ECO:0000269|PubMed:10747962,
CC ECO:0000269|PubMed:15679845, ECO:0000269|PubMed:8951083}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC47311.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U48298; AAC47311.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AAFI02000007; EAL71482.1; -; Genomic_DNA.
DR RefSeq; XP_645420.1; XM_640328.1.
DR AlphaFoldDB; P54681; -.
DR STRING; 44689.DDB0185120; -.
DR PaxDb; P54681; -.
DR EnsemblProtists; EAL71482; EAL71482; DDB_G0271916.
DR GeneID; 8618200; -.
DR KEGG; ddi:DDB_G0271916; -.
DR dictyBase; DDB_G0271916; rtoA.
DR eggNOG; ENOG502RC1Z; Eukaryota.
DR HOGENOM; CLU_755285_0_0_1; -.
DR OMA; ATSDMAN; -.
DR PRO; PR:P54681; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0031210; F:phosphatidylcholine binding; IDA:dictyBase.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0048388; P:endosomal lumen acidification; IMP:dictyBase.
DR GO; GO:0006887; P:exocytosis; IMP:dictyBase.
DR GO; GO:0006972; P:hyperosmotic response; IEP:dictyBase.
DR GO; GO:0061025; P:membrane fusion; IDA:dictyBase.
DR GO; GO:0045595; P:regulation of cell differentiation; IMP:dictyBase.
DR GO; GO:0006885; P:regulation of pH; IMP:dictyBase.
DR GO; GO:0009408; P:response to heat; IEP:dictyBase.
DR GO; GO:0006970; P:response to osmotic stress; IMP:dictyBase.
DR GO; GO:0006979; P:response to oxidative stress; IEP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0006906; P:vesicle fusion; IMP:dictyBase.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Repeat; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..367
FT /note="Protein rtoA"
FT /id="PRO_0000097518"
FT REPEAT 147..156
FT /note="1; truncated"
FT REPEAT 157..167
FT /note="2; truncated"
FT REPEAT 168..178
FT /note="3"
FT REPEAT 179..189
FT /note="4"
FT REPEAT 190..200
FT /note="5"
FT REPEAT 201..211
FT /note="6"
FT REPEAT 212..222
FT /note="7"
FT REPEAT 223..233
FT /note="8"
FT REPEAT 234..244
FT /note="9"
FT REPEAT 245..254
FT /note="10; truncated"
FT REPEAT 255..265
FT /note="11"
FT REGION 24..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147
FT /note="11 X 11 AA tandem repeats of G-S(2)-N-S-G-S-E-S(3)"
FT CONFLICT 165..167
FT /note="GST -> SS (in Ref. 1; AAC47311)"
FT /evidence="ECO:0000305"
FT CONFLICT 352
FT /note="L -> V (in Ref. 1; AAC47311)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 367 AA; 35423 MW; E93657F34AB90DA5 CRC64;
MKLIAFIAFL LILITIINAQ GSIGSSSQSL SSEVDSSDIS SSGSNSTASS EGSVSSSSNS
GSQSTSNSGS EASGSSNSGS QSTSNSGSEA SGSSNSGSQS STDSSNSGSQ GSTGSSNSGS
QSSTDSSNSG SQSSTDSSNS GSQGSTGSSN SGSESSGSSN SGSEGSTGSS NSGSESSSGS
SNSGSESSSG SSNSGSESSS GSSNSGSESS SGSSNSGSES SSGSSNSGSE SSSGSSNSGS
ESSSGSSNSG SESSGSSNSG SESSSDSGSS SDGKTTCISF HDTLSINTVD DDEIECTGKG
ETRCISDNNY KCATKQRHGS IECSVNGYIR CTGSNIKCKI GNTECSIRNS RLDITEKPIF
TRGPRHH
