ID   BCRB_ENTFL              Reviewed;         249 AA.
AC   Q5WNX1;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=Bacitracin transport permease protein BcrB {ECO:0000305};
GN   Name=bcrB {ECO:0000303|PubMed:15388429};
GN   ORFNames=BHU49_14280 {ECO:0000312|EMBL:OGX76143.1};
OS   Enterococcus faecalis (Streptococcus faecalis).
OG   Plasmid pJM01.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=AR01/DGVS;
RX   PubMed=15388429; DOI=10.1128/aac.48.10.3743-3748.2004;
RA   Manson J.M., Keis S., Smith J.M.B., Cook G.M.;
RT   "Acquired bacitracin resistance in Enterococcus faecalis is mediated by an
RT   ABC transporter and a novel regulatory protein, BcrR.";
RL   Antimicrob. Agents Chemother. 48:3743-3748(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Enfs51;
RA   Thong K.L., Tan S.C.;
RT   "Comparative genome analysis of Enterococcus faecalis.";
RL   Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INDUCTION.
RC   STRAIN=AR01/DGVS;
RX   PubMed=18227063; DOI=10.1074/jbc.m709503200;
RA   Gauntlett J.C., Gebhard S., Keis S., Manson J.M., Pos K.M., Cook G.M.;
RT   "Molecular analysis of BcrR, a membrane-bound bacitracin sensor and DNA-
RT   binding protein from Enterococcus faecalis.";
RL   J. Biol. Chem. 283:8591-8600(2008).
CC   -!- FUNCTION: Essential for high-level bacitracin resistance
CC       (PubMed:15388429). Part of the ABC transporter complex BcrAB. Probably
CC       responsible for the translocation of the substrate across the membrane
CC       (Probable). {ECO:0000269|PubMed:15388429, ECO:0000305|PubMed:15388429}.
CC   -!- SUBUNIT: The complex is probably composed of two ATP-binding proteins
CC       (BcrA) and two transmembrane proteins (BcrB). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Transcription is activated by the regulatory protein BcrR in
CC       the presence of bacitracin. {ECO:0000269|PubMed:15388429,
CC       ECO:0000269|PubMed:18227063}.
CC   -!- DISRUPTION PHENOTYPE: Disruption mutant is sensitive to bacitracin.
CC       {ECO:0000269|PubMed:15388429}.
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DR   EMBL; AY496968; AAS78450.1; -; Genomic_DNA.
DR   EMBL; MJEK01000021; OGX76143.1; -; Genomic_DNA.
DR   RefSeq; WP_002367750.1; NZ_VWNQ01000021.1.
DR   AlphaFoldDB; Q5WNX1; -.
DR   SMR; Q5WNX1; -.
DR   EnsemblBacteria; OGX76143; OGX76143; BHU49_14280.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Antibiotic resistance; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..249
FT                   /note="Bacitracin transport permease protein BcrB"
FT                   /id="PRO_0000447367"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   249 AA;  27788 MW;  1A36D0AF3717CF49 CRC64;
     MLNLISCELS KLKRSKMVLI SVAGVLSTPL LMLIEALQTH FDKPEIIFTL SDIYSDSVLY
     IMLLVNMMIY VAIAAYLYSR EYTENTLKTI LPIPISRTKL LIGKFCTLLL WIVMLTLVTW
     AGIFIVCGLY HVVFTLEGYS LLVAISWLPK FLFGGILMFL TTSPFVFIAF KTKGFVAPVI
     ASAVIVMGSV ALSNQELGAL YPWTATFFLI DGRIESTGYP LALAIGIIIL VSAVGFFMTF
     HHFKKEDLK