RTRAF_MOUSE
ID RTRAF_MOUSE Reviewed; 244 AA.
AC Q9CQE8;
DT 03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=RNA transcription, translation and transport factor protein {ECO:0000312|MGI:MGI:1915295};
GN Name=RTRAF {ECO:0000312|MGI:MGI:1915295};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: RNA-binding protein involved in modulation of mRNA
CC transcription by Polymerase II. Component of the tRNA-splicing ligase
CC complex and is required for tRNA ligation. May be required for RNA
CC transport. {ECO:0000250|UniProtKB:Q9Y224}.
CC -!- SUBUNIT: Homodimer. Interacts with FAM98A (via N- and C-terminus).
CC Interacts with NIN; which may prevent phosphorylation of NIN. Interacts
CC with POLR2A. Component of a tRNA-splicing ligase complex.
CC {ECO:0000250|UniProtKB:Q9Y224}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y224}.
CC Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9Y224}. Cytoplasm,
CC perinuclear region {ECO:0000250|UniProtKB:Q9Y224}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q9Y224}. Note=May localize at the centrosome
CC during mitosis. Shuttles between the cytosol and the nucleus: enters
CC into the nucleus in case of active transcription while it accumulates
CC in cytosol when transcription level is low (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RTRAF family. {ECO:0000305}.
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DR EMBL; AK003732; BAB22966.1; -; mRNA.
DR EMBL; AK012460; BAB28255.1; -; mRNA.
DR EMBL; AK012486; BAB28273.1; -; mRNA.
DR EMBL; BC048357; AAH48357.1; -; mRNA.
DR CCDS; CCDS26839.1; -.
DR RefSeq; NP_080804.1; NM_026528.3.
DR AlphaFoldDB; Q9CQE8; -.
DR SMR; Q9CQE8; -.
DR BioGRID; 212624; 43.
DR IntAct; Q9CQE8; 6.
DR MINT; Q9CQE8; -.
DR STRING; 10090.ENSMUSP00000022341; -.
DR iPTMnet; Q9CQE8; -.
DR PhosphoSitePlus; Q9CQE8; -.
DR SwissPalm; Q9CQE8; -.
DR REPRODUCTION-2DPAGE; Q9CQE8; -.
DR EPD; Q9CQE8; -.
DR jPOST; Q9CQE8; -.
DR MaxQB; Q9CQE8; -.
DR PaxDb; Q9CQE8; -.
DR PRIDE; Q9CQE8; -.
DR ProteomicsDB; 262725; -.
DR Antibodypedia; 10723; 317 antibodies from 27 providers.
DR DNASU; 68045; -.
DR Ensembl; ENSMUST00000022341; ENSMUSP00000022341; ENSMUSG00000021807.
DR GeneID; 68045; -.
DR KEGG; mmu:68045; -.
DR UCSC; uc007siv.1; mouse.
DR CTD; 51637; -.
DR MGI; MGI:1915295; Rtraf.
DR VEuPathDB; HostDB:ENSMUSG00000021807; -.
DR eggNOG; KOG4380; Eukaryota.
DR GeneTree; ENSGT00390000005163; -.
DR HOGENOM; CLU_075085_0_0_1; -.
DR InParanoid; Q9CQE8; -.
DR OMA; NRKEFAS; -.
DR OrthoDB; 1239557at2759; -.
DR PhylomeDB; Q9CQE8; -.
DR TreeFam; TF323606; -.
DR BioGRID-ORCS; 68045; 21 hits in 72 CRISPR screens.
DR PRO; PR:Q9CQE8; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q9CQE8; protein.
DR Bgee; ENSMUSG00000021807; Expressed in bone fossa and 246 other tissues.
DR ExpressionAtlas; Q9CQE8; baseline and differential.
DR Genevisible; Q9CQE8; MM.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0072686; C:mitotic spindle; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0072669; C:tRNA-splicing ligase complex; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0000993; F:RNA polymerase II complex binding; ISS:UniProtKB.
DR GO; GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; ISS:UniProtKB.
DR InterPro; IPR019265; RTRAF.
DR PANTHER; PTHR15924; PTHR15924; 1.
DR Pfam; PF10036; RLL; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Cytoskeleton; Nucleus; Reference proteome;
KW RNA-binding; Transcription; Transcription regulation.
FT CHAIN 1..244
FT /note="RNA transcription, translation and transport factor
FT protein"
FT /id="PRO_0000089957"
FT MOD_RES 20
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y224"
FT MOD_RES 62
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y224"
FT MOD_RES 98
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y224"
SQ SEQUENCE 244 AA; 28152 MW; 973961CDEB52BBDB CRC64;
MFRRKLTALD YHNPSGFNCK DETEFRNFIV WLEDQKIRHY KIEDRGNLRN IHSSDWPKFF
EKYLKDVNCP FKIQDRQEAI DWLLGLAVRL EYGDNAEKYK DLVPDNRKNT DNAAKNAEPL
INLDVNNPDF KAGVMALANL LQIQRHDDYL VMLKAIRILV QERLTQDAVA KANQTKEGLP
VALEKHILGF DTGDAVLNEA AQILRLLHIE ELRELQTKIN EAIVAVQAII ADPKTDHRLG
KVGR
