RTTN_DROME
ID RTTN_DROME Reviewed; 1977 AA.
AC A1Z8X3;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Protein rotatin homolog {ECO:0000305};
DE AltName: Full=Anastral spindle 3 {ECO:0000312|FlyBase:FBgn0266111};
GN Name=ana3 {ECO:0000312|FlyBase:FBgn0266111};
GN ORFNames=CG13162 {ECO:0000312|FlyBase:FBgn0266111};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=19948479; DOI=10.1083/jcb.200905031;
RA Stevens N.R., Dobbelaere J., Wainman A., Gergely F., Raff J.W.;
RT "Ana3 is a conserved protein required for the structural integrity of
RT centrioles and basal bodies.";
RL J. Cell Biol. 187:355-363(2009).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH RCD4.
RX PubMed=32543652; DOI=10.1083/jcb.201912154;
RA Panda P., Kovacs L., Dzhindzhev N., Fatalska A., Persico V., Geymonat M.,
RA Riparbelli M.G., Callaini G., Glover D.M.;
RT "Tissue specific requirement of Drosophila Rcd4 for centriole duplication
RT and ciliogenesis.";
RL J. Cell Biol. 219:0-0(2020).
CC -!- FUNCTION: Participes in the structural integrity of both centrioles and
CC basal bodies and in centriole cohesion (PubMed:19948479). Participates
CC in the later stages of centriole assembly through the interaction with
CC Rcd4 leading to the centriole to centrosome conversion
CC (PubMed:32543652). {ECO:0000269|PubMed:19948479,
CC ECO:0000269|PubMed:32543652}.
CC -!- SUBUNIT: Interacts with Rcd4;this complex is recruited to daughter
CC centrioles before their conversion to centrosomes.
CC {ECO:0000269|PubMed:32543652}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000269|PubMed:19948479,
CC ECO:0000269|PubMed:32543652}. Note=Associated with the daughter
CC centriole on mitotic entry (PubMed:32543652). Ana3 recruitment to the
CC procentriole in interphase precedes the Rcd4 one (PubMed:32543652).
CC Localizes to the basal bodies (PubMed:19948479).
CC {ECO:0000269|PubMed:19948479, ECO:0000269|PubMed:32543652}.
CC -!- SIMILARITY: Belongs to the rotatin family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58528.2; -; Genomic_DNA.
DR RefSeq; NP_610765.1; NM_136921.2.
DR AlphaFoldDB; A1Z8X3; -.
DR SMR; A1Z8X3; -.
DR IntAct; A1Z8X3; 2.
DR STRING; 7227.FBpp0087015; -.
DR PaxDb; A1Z8X3; -.
DR PRIDE; A1Z8X3; -.
DR EnsemblMetazoa; FBtr0087904; FBpp0087015; FBgn0266111.
DR GeneID; 36341; -.
DR KEGG; dme:Dmel_CG13162; -.
DR UCSC; CG13162-RA; d. melanogaster.
DR CTD; 36341; -.
DR FlyBase; FBgn0266111; ana3.
DR VEuPathDB; VectorBase:FBgn0266111; -.
DR eggNOG; ENOG502QPM7; Eukaryota.
DR GeneTree; ENSGT00640000091535; -.
DR HOGENOM; CLU_232193_0_0_1; -.
DR InParanoid; A1Z8X3; -.
DR OMA; MINCCSC; -.
DR OrthoDB; 846261at2759; -.
DR PhylomeDB; A1Z8X3; -.
DR BioGRID-ORCS; 36341; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36341; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0266111; Expressed in cleaving embryo and 17 other tissues.
DR Genevisible; A1Z8X3; DM.
DR GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR GO; GO:0005813; C:centrosome; IEA:InterPro.
DR GO; GO:0036064; C:ciliary basal body; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0061823; C:ring centriole; IDA:UniProtKB.
DR GO; GO:0098534; P:centriole assembly; IMP:UniProtKB.
DR GO; GO:0007099; P:centriole replication; IMP:FlyBase.
DR GO; GO:0010457; P:centriole-centriole cohesion; IMP:FlyBase.
DR GO; GO:0007098; P:centrosome cycle; IMP:FlyBase.
DR GO; GO:0032053; P:ciliary basal body organization; IMP:FlyBase.
DR InterPro; IPR030791; Rotatin.
DR InterPro; IPR029249; Rotatin_N.
DR PANTHER; PTHR31691; PTHR31691; 1.
DR Pfam; PF14726; RTTN_N; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..1977
FT /note="Protein rotatin homolog"
FT /id="PRO_0000454387"
FT REGION 141..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1977 AA; 225556 MW; 155A0E2A98F22B7C CRC64;
MSTKQSPALQ LAEGQLTKLT SESEEIRMRA LDQIETRFIR CLQLGEPIQF KPVLLLKQLI
RWFGYTPPLV PDRVLAMIME LLRSEYAEAV IRKIPYERFK AELQKVRRVL HKQESKRVSE
LLDDMNLLLL EKYNIDRVTP SVSSLSSNDI PSQATESADS SSNQIYDNLK PEDYEPSWSH
PCLDDVATMK SMIDLPRNSV ELQLQLTELI IRMGDYPTEY FLQPPFVFLH LVQLQTMTDG
SLIHVNRALI ACLRLLQQRI LLRRNTLSYA DRFDPPSRPK QVKVVSALVI LLENCMKLIR
PLLFSCTNDN WHIMELIVEI VRTHDVLSSK IPLVSITLIA DAVKSLLAYC NSVEGSCMTQ
LMDSLRIPRL QSLILNGVLH DMVALNINYD KRMDRRQAKA LIQPIVLDSA YLSGMPERMK
SLNTLISSLD SGPSPDEELI KLKRAYSVAL NQLHPNTELT GSLLIQKYRQ VCLVLVQLGS
ETLVKQLFGA VVKCIPFYAG NLKLRKDADE LLYTLVDLPA LKLRSLIYRL MIKSTVAHFH
SFMNKTVYMT GCSNVDLIRQ HILGVPLTPL LLRRMIIQSS EANTSERMQQ WCLDYPIMIM
KLNAILAPQD FSVVFPLLLP VLPLLISRSV THKILHNVIW NVLEPDSSRL DPQLMLRGYV
YFMFHPDGEV RSEATTTIAY VLQCQEQTNR YLPTASNVPV EHIANDLCII QPPFCYRSIF
IKCSDERFQG QRSLDALFRL LQAKDIKSNI RKSTMTQLNV LLRNWRACEE FSTKEDGYRL
IMESLHNALK KGNDTDILLP TVSILMKLLF NDDEFRLEVA NTFGVYTLLL RALFLFPHLE
QLRQDVSICL FQMLFQNCIT STEDKLVLNA NMEPLILPVT YEIEHKVIPT AVTEGLELQQ
NVEATHFGRD RAKAAQHWRL YMAYRVCQVP SSITLESVSA LDIRESLKIK MADLALVRSS
DLNEQLSCQF IAAENCSKHE DLQKTVSAIQ LFLVVLRNSL SDTVAENLWK LIHKYIRLAP
GNEADDKVYK SMLDLCVTCI RFSQPHAING LSYALETDHH HSFQLLLHDS QISLDKLFLI
CQCMMQLLSN ELSDDSMNWY GKFFMQLSAV AKTHFELRQL QHVRCILCML RFVSERNLKF
SNAQLMSYSQ HFIQLSSNLR TSTQTGSEWQ RDCLYIICQL QIHLIYQEPK ASSKASIPND
VGASYKVLRY FLTLCGHGDS EVRALSWVSL ANWITICGSQ VAEILPRLDF LPGGLPACCL
TTLTDAHEMM LIRELAGRVF ILLMPLIGAE SSLELLRKHD LLKTAYNSLK AIHDTPWMFK
KIVGERHSCE VISCYVAICT KMVAMKPEWC AALCGHSLMS GLSDVMKKLK SQASCSIPLV
ELCASQICEL YSMCYTNNFE FLKMTICRDS VFLQNYLTLI NDVLSLECPE YMVIPLLKMF
LIFCTDSNSN SFLIEQIKNK PSLFMDFFLF GLHVKLINSP FQRFTLSALS LVFTKAQLAA
YDISMLSELE KFELAFSDPD IAKDGKQEFE SKKKQSVSQC IYDESSDNSD DNKQRPNHAI
QATNAAIIIF HRLDQLFDRY YLSKALNFLE LPAVGQVQVC EALGELLKAS TWAVKAAGES
KLLGKVVHIL DDFLNDEKIG NAAVYVKRVG PHKAQSIISN LLVLINMLSQ WHSSPNSEII
QTSMAANIAK ILIRIWPWLS HSYHLKKVTV QLIMFLTEHS FEMCKQISLL QSGHAQSLLH
LMARVADFET TKKEIPNKEP SLNMVPALRV MVNCCCCTEG RLSLSKMNLL DMFDTILPAN
PCSTHLKVRP PVLIAWLGFW EVFSRYDVGG KACHLQSLIN TIRRSPPLSH KRILCLRILR
NMCFFNGNRP RLVELADFIN LLRDILEQRV QKAPTSEKNG LNSFEEHRLA VLMLWKLFGF
GAKYKGMLRG TKLFKLLIGL RVEMSVVFSE EENKYAGVPY ANDLAKLLEK LMESMRQ
