RTTN_MOUSE
ID RTTN_MOUSE Reviewed; 2226 AA.
AC Q8R4Y8; A0PJL2; Q8BPW5; Q8C461; Q8CCK5; Q8CIG4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Rotatin {ECO:0000305};
GN Name=Rttn {ECO:0000312|MGI:MGI:2179288};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Eye, Olfactory bulb, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-2034 (ISOFORM 1), DISRUPTION PHENOTYPE, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=11900971; DOI=10.1016/s0925-4773(02)00003-5;
RA Faisst A.M., Alvarez-Bolado G., Treichel D., Gruss P.;
RT "Rotatin is a novel gene required for axial rotation and left-right
RT specification in mouse embryos.";
RL Mech. Dev. 113:15-28(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1412-2226 (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=17551791; DOI=10.1007/s00335-007-9023-7;
RA Chatterjee B., Richards K., Bucan M., Lo C.;
RT "Nt mutation causing laterality defects associated with deletion of
RT rotatin.";
RL Mamm. Genome 18:310-315(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in the genetic cascade that governs left-right
CC specification. Required for correct asymmetric expression of NODAL,
CC LEFTY and PITX2 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with PPP1R35; this interaction allows the mutual
CC recruitment to the centriole. {ECO:0000250|UniProtKB:Q86VV8}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC {ECO:0000250}. Note=Colocalizes with the basal bodies at the primary
CC cilium. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8R4Y8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R4Y8-2; Sequence=VSP_029034, VSP_029035;
CC Name=3;
CC IsoId=Q8R4Y8-3; Sequence=VSP_029032, VSP_029038, VSP_029039;
CC Name=4;
CC IsoId=Q8R4Y8-4; Sequence=VSP_029033, VSP_029036, VSP_029037;
CC -!- DEVELOPMENTAL STAGE: Expressed at 7.5 dpc. At 8.5 dpc, expressed in
CC telencephalon and somites. At 9.5 dpc, expressed in somites, forelimb
CC bud, basal forebrain and first branchial arch. At 11.5 dpc, expressed
CC in heart primordium and branchial arches.
CC {ECO:0000269|PubMed:11900971}.
CC -!- DISRUPTION PHENOTYPE: Death at 11.5 dpc due to major developmental
CC defects, including notochord degeneration, imperfect differentiation of
CC somites and neural tube, axial rotation failure and randomized heart
CC looping. {ECO:0000269|PubMed:11900971, ECO:0000269|PubMed:17551791}.
CC -!- SIMILARITY: Belongs to the rotatin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH23916.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK032605; BAC27946.1; -; mRNA.
DR EMBL; AK052059; BAC34842.1; -; mRNA.
DR EMBL; AK083016; BAC38732.1; -; mRNA.
DR EMBL; AF265232; AAM02906.1; -; mRNA.
DR EMBL; BC023916; AAH23916.1; ALT_INIT; mRNA.
DR EMBL; BC049935; AAH49935.1; -; mRNA.
DR CCDS; CCDS29390.1; -. [Q8R4Y8-1]
DR RefSeq; NP_780751.2; NM_175542.3. [Q8R4Y8-1]
DR AlphaFoldDB; Q8R4Y8; -.
DR SMR; Q8R4Y8; -.
DR STRING; 10090.ENSMUSP00000023828; -.
DR iPTMnet; Q8R4Y8; -.
DR PhosphoSitePlus; Q8R4Y8; -.
DR EPD; Q8R4Y8; -.
DR MaxQB; Q8R4Y8; -.
DR PaxDb; Q8R4Y8; -.
DR PeptideAtlas; Q8R4Y8; -.
DR PRIDE; Q8R4Y8; -.
DR ProteomicsDB; 260868; -. [Q8R4Y8-1]
DR ProteomicsDB; 260869; -. [Q8R4Y8-2]
DR ProteomicsDB; 260870; -. [Q8R4Y8-3]
DR ProteomicsDB; 260871; -. [Q8R4Y8-4]
DR Antibodypedia; 49105; 26 antibodies from 9 providers.
DR DNASU; 246102; -.
DR Ensembl; ENSMUST00000023828; ENSMUSP00000023828; ENSMUSG00000023066. [Q8R4Y8-1]
DR GeneID; 246102; -.
DR KEGG; mmu:246102; -.
DR UCSC; uc008fvi.1; mouse. [Q8R4Y8-2]
DR UCSC; uc008fvj.1; mouse. [Q8R4Y8-1]
DR UCSC; uc008fvk.1; mouse. [Q8R4Y8-4]
DR UCSC; uc012bfr.1; mouse. [Q8R4Y8-3]
DR CTD; 25914; -.
DR MGI; MGI:2179288; Rttn.
DR VEuPathDB; HostDB:ENSMUSG00000023066; -.
DR eggNOG; ENOG502QPM7; Eukaryota.
DR GeneTree; ENSGT00640000091535; -.
DR HOGENOM; CLU_001318_0_0_1; -.
DR InParanoid; Q8R4Y8; -.
DR OMA; IICICSK; -.
DR OrthoDB; 846261at2759; -.
DR PhylomeDB; Q8R4Y8; -.
DR TreeFam; TF323508; -.
DR BioGRID-ORCS; 246102; 6 hits in 71 CRISPR screens.
DR ChiTaRS; Rttn; mouse.
DR PRO; PR:Q8R4Y8; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q8R4Y8; protein.
DR Bgee; ENSMUSG00000023066; Expressed in animal zygote and 181 other tissues.
DR ExpressionAtlas; Q8R4Y8; baseline and differential.
DR Genevisible; Q8R4Y8; MM.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; ISS:MGI.
DR GO; GO:0007099; P:centriole replication; IBA:GO_Central.
DR GO; GO:0010457; P:centriole-centriole cohesion; IBA:GO_Central.
DR GO; GO:0032053; P:ciliary basal body organization; IBA:GO_Central.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR030791; Rotatin.
DR InterPro; IPR029249; Rotatin_N.
DR PANTHER; PTHR31691; PTHR31691; 1.
DR Pfam; PF14726; RTTN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 3.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell projection; Cilium; Cytoplasm;
KW Cytoskeleton; Developmental protein; Phosphoprotein; Reference proteome.
FT CHAIN 1..2226
FT /note="Rotatin"
FT /id="PRO_0000308613"
FT REGION 295..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..346
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 311
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VV8"
FT MOD_RES 813
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q86VV8"
FT VAR_SEQ 1..1494
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029032"
FT VAR_SEQ 1..622
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029033"
FT VAR_SEQ 602
FT /note="I -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029034"
FT VAR_SEQ 603..2226
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029035"
FT VAR_SEQ 863..906
FT /note="DIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLR -> GNED
FT FVLFCSVLFCSVLFCSVLSCFVLFCFLTVIVAHLGFEIAV (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029036"
FT VAR_SEQ 907..2226
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029037"
FT VAR_SEQ 1916..1921
FT /note="KEDGFI -> KGGWLH (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029038"
FT VAR_SEQ 1922..2226
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029039"
FT CONFLICT 1420
FT /note="V -> M (in Ref. 3; AAH23916)"
FT /evidence="ECO:0000305"
FT CONFLICT 2034
FT /note="M -> I (in Ref. 2; AAM02906)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2226 AA; 248489 MW; B962D993E7E8BE4A CRC64;
MVLAGLIRKL GHQLAEIRER ALKSILCKIE HNLICYADLI QERLLFLHLL EWFNFPSVPM
KEEVLNLLSR LVKYSPAVQH LIDLGAVEFL SKLRPNVEPV IQAEIDGILD GLFILPSEVP
ALCSSSYHTD QIELSQQPEV LTGYFPQDKS NFQQMEVPPG PAGNQAVKWL RFSVFPWLPL
TTTDRHVLSS NESSLRSSNH TLIWNTCELL KDVIMQDFPA EIFLQRPKIV QGLLCLVKLA
FGGDGKHRLA LQSVSCLQQL CTSLRNRLNF YRDPNFFSSR QDTVSQNSSL SYCHEARGPY
HSPNPSPGSS SSRPSVVGRT GQRPRGDGQD WDAVSSSGSS SHTHVNSRIS VHSPLDVAPV
DLPELEAEDT LELQFQQLSL PQFCISTLEA AAPLLRTGSR EMIIRVLELL AEDMLLIGQA
ISSEIWDDNS LFAINVKEKL LQVLGSLGET MCYRKTSFSM EQAEAVLVHH RMAFVSISLF
TVRLLQLLLP VEKASEFLPE SMSAALFFVS LDMPISLEYP EIHDTVVAYL EQLDSENYSI
YKRTAEAVYS IECTCNFLSD VGKEGEKNLL ELVELADQAL RSFSYHQHSP LIKEIICICS
KIWKSAQASP LLQGESQKVF LHMLSHPLPQ VKVETYQCCL EIVKECLGVQ NVTKPVSSLC
SGIHFLLHPK VLYEISAFGI QESKNEVNAA AKAILLYLLQ GRLMMTALTW NKFIESLAPV
IPVLQGYADT EDPLGNCILL LSKVSSEAGE GALPCTARLK SVLRLLLVKK PSVRSLALKL
LAFHLTSEEG ADKKRPFIDA GVLSRVTNLF IVKKPIDLKL DDRRELVIKL ETVKKVCDIF
TSDDVDLVLR KSAAEQLAVI MQDIKMHTVV KKLCLVEKII GHLNEFVGQD GEVIECLIQP
CLTLLRKVLY ADPVIRVSLS QQASLLTLLL RVSLIFHEDC TVVAEVGALF CLLLFDEVSR
MDTWSDTLSD KPSLSSVFSL PVSVFRRYHL PVHVTGHHAV SPYSIVLPLP TECLTLKPVS
DMLRIAWNLA WYHGSDNLLK QMTSEAKIQE FLDTLQLSTE DILALKITYT DSGLKDCLQS
IIQAGGHRDV RAAITRMSFY LLNDKLSLKD DIGPCGVTLK SLAWHTVLNR FLQVLPACTE
DEKLLIDIIH FLNKLIKEQR KNPSIELLNW ILELLLRHNP SPLLDLLVLT DSHAGEETDD
VRTAVRQQLQ KELISLLNSL LLVFMTVSDR KCLELFYVFQ TQLSLKLLQC LRVTDAPHFY
GLPSLERTLA GMAHLTAFPG WSAHSPLSKP LEICGKYLSG LLEVITSFYV ERGGNAMSFM
GKGVTKSTGL CLLHLSHEMI VQAPGSEWMP LWFLPLGSHS EEHAPTQQGL AWLIPLWVDR
DPEVRFTSLG LGSALTTIEA GCVALANSCQ NISGGLWGTV VNILLDQSEC SLVRREAAFI
LQNLLVIPMP SEIIKDYTWQ GPCVHDEDSG LSLSGKPALQ ALLYHCRFYE HLTHMVKHCY
VGRYIFDFNF SAFTETSEYS DLNGLDDSFK FWRAPSGTST QDHEPSSLST SETMVAASVV
SSEIQPLLPS TLLPETALDQ FVTQGQREMR PVRPRDSLLS TSLNRQYVFV TPSLLSAVCS
LLDNLLAVTP RDAANAFQQA HLIELLCSTV DASLIEMCIQ ELKTPLPLPS AVEHIQAQAS
FFLEYLSSLS QLLKSCLLVE PKLVTQDELL KPLITNVIRV LIVCPKDVLD VQLVLAFYRT
WTHLFDLLAT LLRKAGAVSL VPLTSALAKH WEAVTDTLCR CVGLSFKCPA LTIASLQFLS
VLLAEEEKRR VQDKDKTNEG QAPTVALLLD GTQGSLSSSE RLNETILQCY EGISPKDVLK
RVAANALLSL LAVSRRAQRH ALRSDLIENC MEQMKHINAQ LNLDLLRPGK AVLKKKEDGF
IKELSITMQL LRNCLYQNEE CKVAALEARL VPVLHSLWPW LLMEDSLMQI ALQLLCVYTA
NFPAGCSSLC WSNYGQCIQT AHRGTPSSSL ILCILKLASQ MPAENTTIQQ LVFMFLSNLA
LSHDCKGVIQ KSNFLQHFLS LTLPKGGNQH LSTVAILWLK LLLNMSFGED GQQMILRLDG
CLDLLIEMSK YKHKSSPHMP LLIFHNICFS PANKPKILAN EKVVTLLAAC LESENQTAQR
IGAASLWALT YNYQKAKATL KNPSIKRKVD EAYSIAKRTL SNSEENPLNS YYLKCLENLV
QLLNYS
