RTX1D_ACTPL
ID RTX1D_ACTPL Reviewed; 477 AA.
AC P26761;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=RTX-I toxin determinant D;
DE AltName: Full=APX-ID;
DE AltName: Full=Cytolysin ID;
DE Short=CLY-ID;
DE AltName: Full=HLY-ID;
DE AltName: Full=Toxin RTX-I secretion protein D;
GN Name=apxID; Synonyms=appD, clyID, hlyID;
OS Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Actinobacillus.
OX NCBI_TaxID=715;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype 5;
RX PubMed=1860823; DOI=10.1128/jb.173.16.5151-5158.1991;
RA Chang Y.-F., Young R., Struck D.K.;
RT "The Actinobacillus pleuropneumoniae hemolysin determinant: unlinked appCA
RT and appBD loci flanked by pseudogenes.";
RL J. Bacteriol. 173:5151-5158(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate CVI 13261 / Serotype 9;
RX PubMed=1937809; DOI=10.1128/iai.59.12.4497-4504.1991;
RA Smits M.A., Briaire J., Jansen R., Smith H.E., Kamp E.M., Gielkens A.L.J.;
RT "Cytolysins of Actinobacillus pleuropneumoniae serotype 9.";
RL Infect. Immun. 59:4497-4504(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 27088 / DSM 13472 / CCM 5869 / S4074 / Serotype 1;
RX PubMed=8181764; DOI=10.1016/0378-1119(94)90361-1;
RA Frey J., Haldimann A., Nicolet J., Boffini A., Prentki P.;
RT "Sequence analysis and transcription of the apxI operon (hemolysin I) from
RT Actinobacillus pleuropneumoniae.";
RL Gene 142:97-102(1994).
CC -!- FUNCTION: Involved in the transport of the toxin RTX-I as well as that
CC of RTX-II.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: The sequence shown is that of serotype 5.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
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DR EMBL; M65808; AAB00967.1; -; Genomic_DNA.
DR EMBL; X61112; CAA43426.1; -; Genomic_DNA.
DR EMBL; X68595; CAA48588.1; -; Genomic_DNA.
DR PIR; E43599; E43599.
DR AlphaFoldDB; P26761; -.
DR SMR; P26761; -.
DR STRING; 228399.appser1_15220; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR InterPro; IPR006144; Secretion_HlyD_CS.
DR InterPro; IPR010129; T1SS_HlyD.
DR TIGRFAMs; TIGR01843; type_I_hlyD; 1.
DR PROSITE; PS00543; HLYD_FAMILY; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cytolysis; Hemolysis; Membrane;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..477
FT /note="RTX-I toxin determinant D"
FT /id="PRO_0000201891"
FT TOPO_DOM 1..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..477
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 14
FT /note="A -> R (in strain: S 4074 / Serotype 1 and Isolate
FT CVI 13261 / Serotype 9)"
FT VARIANT 94
FT /note="G -> D (in strain: S 4074 / Serotype 1 and Isolate
FT CVI 13261 / Serotype 9)"
FT VARIANT 113
FT /note="E -> Q (in strain: S 4074 / Serotype 1 and Isolate
FT CVI 13261 / Serotype 9)"
FT VARIANT 228
FT /note="S -> I (in strain: S 4074 / Serotype 1 and Isolate
FT CVI 13261 / Serotype 9)"
FT VARIANT 335
FT /note="Y -> GT (in strain: S 4074 / Serotype 1 and Isolate
FT CVI 13261 / Serotype 9)"
SQ SEQUENCE 477 AA; 54728 MW; B841D87AF7D4D4C2 CRC64;
MKTWLMGLYE FFQAYKTVWT EIWKIRHQLD TPDREKDENE FLPAHLELIE TPVSKKPRLI
AYLIMLFLFL ALVISIVSHV EIVATATGKL AFSGRSKEIK PIENALVKEI FVEDGQFVEK
DQLLLHLTAL GADADQQKTK SSLSLTKLER YRYEILLEAV AADRLPLIEL TKDEFKHATE
EDKTRIRYLI TEQFEAWQKQ KYQKELALQR REAEKQTVLA NIRKYEGSSR VENERLKDLK
KLFNSKSTSK HDVLTQENRH IEAVNELAVY KSRLNEVESD LRQAKEEIHL ITQLFRADIL
EKLKQNVEAE KQLSLELEKN EQRQIASVIR APVSYVQQLK THTVGGVVTT AETLMVIAPE
DDVLEVTALI QNKDIGFIEV GQDAVIKVET FPYTRYGYLM GKVKNITLEA IEHPQLGLVF
NSIISIDRKT LSGKDGKEIE LGSGMSVTAE IKTGERSVIS YLLSPLEESV SESLRER
