ID   RTXE_DROME              Reviewed;         908 AA.
AC   Q9NBX4;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Probable RNA-directed DNA polymerase from transposon X-element;
DE            EC=2.7.7.49;
DE   AltName: Full=Reverse transcriptase;
GN   Name=X-element\ORF2; Synonyms=ORF2;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1] {ECO:0000312|EMBL:AAF81411.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11405632; DOI=10.1007/s004380000437;
RA   Tudor M., Davis A.J., Feldman M., Grammatikaki M., O'Hare K.;
RT   "The X element, a novel LINE transposable element from Drosophila
RT   melanogaster.";
RL   Mol. Genet. Genomics 265:489-496(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49;
CC         Evidence={ECO:0000250|UniProtKB:P21328, ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- MISCELLANEOUS: At least 30 copies of X-element are present in the
CC       genome and some elements are polymorphic between different strains.
CC       {ECO:0000250|UniProtKB:P21328}.
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DR   EMBL; AF237761; AAF81411.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9NBX4; -.
DR   SMR; Q9NBX4; -.
DR   FlyBase; FBgn0041612; X-element\ORF2.
DR   PRO; PR:Q9NBX4; -.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IMP:UniProtKB.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IMP:UniProtKB.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR000477; RT_dom.
DR   Pfam; PF14529; Exo_endo_phos_2; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   3: Inferred from homology;
KW   Nucleotidyltransferase; RNA-directed DNA polymerase; Transferase;
KW   Transposable element.
FT   CHAIN           1..908
FT                   /note="Probable RNA-directed DNA polymerase from transposon
FT                   X-element"
FT                   /id="PRO_0000076306"
FT   DOMAIN          481..752
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   REGION          883..908
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   908 AA;  102756 MW;  DB72F180D47D7564 CRC64;
     MMPLRILVWN ADGVSTKLPE VECFVRRHEI DVLLLSETHC KGAETPKLFG FVAYTANDPS
     GGNAKGGAAI LIKNSLAHFP LTPIATAKVQ LAPAVIETAL GPISFGAVYC PPRFAWTTDE
     FKDILEEFQT KFIVAGDWNA SHWLWGAGRS NQRGIALANL VLNSEVDSLA TGGPTRYPYG
     CRGSPGYIDF ALTKGVLGIH ANISAVVELS SDHLPLVITL DAGAISYPKM ERLITRRTNL
     EVFQSQLEST LPLNTAINSG QDVDDAIELL TNNIKSAARL ATRSISRQPA ADRIPIPREI
     LLLIAEKRRL RTRWMRSRHP SDKTEWNRAL SRLRCALVLH KAAWFDERLA NTGVESEATH
     SLWKATRAIK RRCTRKAPLV DSNGTWCRTD LGQAEVFAAH LAERFQPFKL ASLQQVEETQ
     DQLNQALQMD MPITPFEPCE VAEVIVRQSN NKAPGHDVIC NATLKALPRQ AILYITLVFN
     AIVRLQYFPY QWKLGIISMI HKPGKPEREP ASYRPISLLP SISKVFERLI AVRIVSIMEA
     QGITPEHQFG FRAGHCTVEQ LHRVVEQILT AYDSKEYCNS LFLDIREAFD RVWHIGLQLK
     IKQTLPAPYF GLLKSYLEGR RFAVRFHSAI STEHNVAAGV PQGSVLGPLL YCLYSHDMPQ
     PDVSLYGKSM LATFADDVCV TYRSRCEHDA ADGIQDFAYR FSEWARRWNI GINSSKSNNV
     CFTLKRRTPP PVYIEEVPVP QPNAAKYLGV LLDRRLTFSK HVTDIRTRLR AKVAKHYWLL
     SSRSKLSLSN KLTIYKQILA PNWKYGCQIW GLACDSHIKR IQAIQNKVAR LITGCEWFVR
     NTTLHRDLKL ATVFDEINKH SSRYHDRLER HRNRLASALN RSRPPRRLNR RQPRDLITRS
     PLTRVRRS