RU1A_ARATH
ID RU1A_ARATH Reviewed; 250 AA.
AC Q39244;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 154.
DE RecName: Full=U1 small nuclear ribonucleoprotein A;
DE Short=U1 snRNP protein A;
GN Name=U1A; OrderedLocusNames=At2g47580; ORFNames=T30B22.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia; TISSUE=Root, and Seedling;
RX PubMed=7556097; DOI=10.1002/j.1460-2075.1995.tb00133.x;
RA Simpson G.G., Clark G.P., Rothnie H.M., Boelens W., van Venrooij W.,
RA Brown J.W.S.;
RT "Molecular characterization of the spliceosomal proteins U1A and U2B'' from
RT higher plants.";
RL EMBO J. 14:4540-4550(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP SUBUNIT, AND GENE FAMILY.
RX PubMed=15987817; DOI=10.1261/rna.2440305;
RA Lorkovic Z.J., Lehner R., Forstner C., Barta A.;
RT "Evolutionary conservation of minor U12-type spliceosome between plants and
RT humans.";
RL RNA 11:1095-1107(2005).
CC -!- FUNCTION: Involved in nuclear pre-mRNA splicing (By similarity). Seems
CC to not be involved in polyadenylation. {ECO:0000250,
CC ECO:0000269|PubMed:7556097}.
CC -!- SUBUNIT: Component of the spliceosome where it is associated with snRNP
CC U1. {ECO:0000269|PubMed:15987817}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RRM U1 A/B'' family. {ECO:0000305}.
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DR EMBL; Z49991; CAA90283.1; -; mRNA.
DR EMBL; AC002535; AAC62852.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10861.1; -; Genomic_DNA.
DR EMBL; AY052663; AAK96567.1; -; mRNA.
DR EMBL; AY059875; AAL24357.1; -; mRNA.
DR EMBL; AY093341; AAM13340.1; -; mRNA.
DR EMBL; AY142070; AAM98334.1; -; mRNA.
DR PIR; S59118; S59118.
DR RefSeq; NP_182280.1; NM_130326.3.
DR AlphaFoldDB; Q39244; -.
DR SMR; Q39244; -.
DR BioGRID; 4706; 2.
DR IntAct; Q39244; 2.
DR STRING; 3702.AT2G47580.1; -.
DR PaxDb; Q39244; -.
DR PRIDE; Q39244; -.
DR ProteomicsDB; 232721; -.
DR EnsemblPlants; AT2G47580.1; AT2G47580.1; AT2G47580.
DR GeneID; 819371; -.
DR Gramene; AT2G47580.1; AT2G47580.1; AT2G47580.
DR KEGG; ath:AT2G47580; -.
DR Araport; AT2G47580; -.
DR TAIR; locus:2061976; AT2G47580.
DR eggNOG; KOG4206; Eukaryota.
DR HOGENOM; CLU_041869_1_1_1; -.
DR InParanoid; Q39244; -.
DR OMA; LKKGWVM; -.
DR OrthoDB; 1608132at2759; -.
DR PhylomeDB; Q39244; -.
DR PRO; PR:Q39244; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q39244; baseline and differential.
DR Genevisible; Q39244; AT.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0005685; C:U1 snRNP; IDA:UniProtKB.
DR GO; GO:0030619; F:U1 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:TAIR.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..250
FT /note="U1 small nuclear ribonucleoprotein A"
FT /id="PRO_0000416926"
FT DOMAIN 18..97
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 177..250
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 108..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..134
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 250 AA; 28074 MW; 64410DBECCDF5E9E CRC64;
MEMQEANQGG GSEVSPNQTI YINNLNEKVK LDELKKSLNA VFSQFGKILE ILAFKTFKHK
GQAWVVFDNT ESASTAIAKM NNFPFYDKEM RIQYAKTKSD VVAKADGTFV PREKRKRHEE
KGGGKKKKDQ HHDSTQMGMP MNSAYPGVYG AAPPLSQVPY PGGMKPNMPE APAPPNNILF
VQNLPHETTP MVLQMLFCQY QGFKEVRMIE AKPGIAFVEF ADEMQSTVAM QGLQGFKIQQ
NQMLITYAKK
