RU2A_DROME
ID RU2A_DROME Reviewed; 265 AA.
AC Q9V4Q8;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Probable U2 small nuclear ribonucleoprotein A';
DE Short=U2 snRNP A';
GN Name=U2A; ORFNames=CG1406;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP INTERACTION WITH THE SMN COMPLEX.
RX PubMed=18621711; DOI=10.1073/pnas.0802287105;
RA Kroiss M., Schultz J., Wiesner J., Chari A., Sickmann A., Fischer U.;
RT "Evolution of an RNP assembly system: a minimal SMN complex facilitates
RT formation of UsnRNPs in Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10045-10050(2008).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24244416; DOI=10.1371/journal.pone.0079048;
RA Klusza S., Novak A., Figueroa S., Palmer W., Deng W.M.;
RT "Prp22 and spliceosome components regulate chromatin dynamics in germ-line
RT polyploid cells.";
RL PLoS ONE 8:E79048-E79048(2013).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27035939; DOI=10.1073/pnas.1513682113;
RA Wu H., Sun L., Wen Y., Liu Y., Yu J., Mao F., Wang Y., Tong C., Guo X.,
RA Hu Z., Sha J., Liu M., Xia L.;
RT "Major spliceosome defects cause male infertility and are associated with
RT nonobstructive azoospermia in humans.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:4134-4139(2016).
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the spliceosome
CC (By similarity). Associated with sn-RNP U2, where it contributes to the
CC binding of stem loop IV of U2 snRNA (By similarity). In the germ line,
CC has a role in oogenesis, by regulating spermatogenesis and nurse cell
CC nuclei chromatin decondensation and dispersal, probably by regulating
CC the splicing of proteins necessary for germline differentiation such as
CC the meiotic protein mei-P26 (PubMed:27035939, PubMed:24244416).
CC {ECO:0000250|UniProtKB:P09661, ECO:0000269|PubMed:24244416,
CC ECO:0000269|PubMed:27035939}.
CC -!- SUBUNIT: Interacts with the SMN complex. {ECO:0000269|PubMed:18621711}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethal (PubMed:27035939). RNAi-mediated
CC knockdown in male germ cells, spermatogonia and surrounding somatic
CC cells results in increased spermatogonia proliferation, failure to
CC complete developmental programs of meiosis and differentiation and
CC ultimately leads to complete male sterility (PubMed:27035939). RNAi-
CC mediated knockdown in female germline results in defective oogenesis
CC (PubMed:24244416). {ECO:0000269|PubMed:24244416,
CC ECO:0000269|PubMed:27035939}.
CC -!- SIMILARITY: Belongs to the U2 small nuclear ribonucleoprotein A family.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAF59207.1; -; Genomic_DNA.
DR EMBL; AY071516; AAL49138.1; -; mRNA.
DR RefSeq; NP_610315.1; NM_136471.4.
DR PDB; 6F4G; X-ray; 1.90 A; A/D=1-176.
DR PDB; 6F4J; X-ray; 1.42 A; A/B=1-176.
DR PDBsum; 6F4G; -.
DR PDBsum; 6F4J; -.
DR AlphaFoldDB; Q9V4Q8; -.
DR SMR; Q9V4Q8; -.
DR BioGRID; 61587; 17.
DR IntAct; Q9V4Q8; 2.
DR STRING; 7227.FBpp0088015; -.
DR PaxDb; Q9V4Q8; -.
DR PRIDE; Q9V4Q8; -.
DR DNASU; 35713; -.
DR EnsemblMetazoa; FBtr0088941; FBpp0088015; FBgn0033210.
DR GeneID; 35713; -.
DR KEGG; dme:Dmel_CG1406; -.
DR UCSC; CG1406-RA; d. melanogaster.
DR CTD; 35713; -.
DR FlyBase; FBgn0033210; U2A.
DR VEuPathDB; VectorBase:FBgn0033210; -.
DR eggNOG; KOG1644; Eukaryota.
DR GeneTree; ENSGT00940000153289; -.
DR HOGENOM; CLU_061027_1_0_1; -.
DR InParanoid; Q9V4Q8; -.
DR OMA; PNYREYM; -.
DR OrthoDB; 1447031at2759; -.
DR PhylomeDB; Q9V4Q8; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 35713; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 35713; -.
DR PRO; PR:Q9V4Q8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033210; Expressed in ovary and 11 other tissues.
DR Genevisible; Q9V4Q8; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0030532; C:small nuclear ribonucleoprotein complex; ISS:FlyBase.
DR GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR GO; GO:0006325; P:chromatin organization; IMP:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR044640; RU2A.
DR PANTHER; PTHR10552; PTHR10552; 1.
DR PROSITE; PS51450; LRR; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Leucine-rich repeat; Nucleus; Reference proteome; Repeat;
KW Ribonucleoprotein; RNA-binding.
FT CHAIN 1..265
FT /note="Probable U2 small nuclear ribonucleoprotein A'"
FT /id="PRO_0000074177"
FT REPEAT 20..41
FT /note="LRR 1"
FT REPEAT 43..64
FT /note="LRR 2"
FT REPEAT 65..86
FT /note="LRR 3"
FT REPEAT 89..110
FT /note="LRR 4"
FT DOMAIN 123..161
FT /note="LRRCT"
FT HELIX 6..11
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 22..25
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 37..40
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 45..48
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 83..85
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 103..109
FT /evidence="ECO:0007829|PDB:6F4J"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 124..127
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 131..138
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 152..163
FT /evidence="ECO:0007829|PDB:6F4J"
FT HELIX 165..174
FT /evidence="ECO:0007829|PDB:6F4J"
SQ SEQUENCE 265 AA; 29744 MW; FB09D8D1D96501A5 CRC64;
MVKLTPELIN QSMQYINPCR ERELDLRGYK IPQIENLGAT LDQFDTIDLS DNDLRKLDNL
PHLPRLKCLL LNNNRILRIS EGLEEAVPNL GSIILTGNNL QELSDLEPLV GFTKLETICL
LINPVSTKPN YREYMAYKFP QLRLLDFRKI KQKDRQAAQE FFRTKQGKDV LKEISRKSKM
SAAAAIAAEA GNGKGRGSEG GRLANPQDMQ RIREAIKRAS SLAEVERLSQ ILQSGQLPDK
FQHEMEAVAQ NGAGHNGSGA VAMEY
