ID   RU2A_SCHPO              Reviewed;         239 AA.
AC   Q9USX8; Q9UU32;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=U2 small nuclear ribonucleoprotein A';
DE            Short=U2 snRNP A';
GN   Name=lea1; ORFNames=SPBC1861.08c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 63-212, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=ATCC 38364 / 968;
RX   PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA   Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA   Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL   Genes Cells 5:169-190(2000).
RN   [3]
RP   IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA   Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT   "Proteomics analysis reveals stable multiprotein complexes in both fission
RT   and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT   splicing factors, and snRNAs.";
RL   Mol. Cell. Biol. 22:2011-2024(2002).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. This protein is associated
CC       with sn-RNP U2. It helps the A' protein to bind stem loop IV of U2
CC       snRNA (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC       spliceosome sub-complex reminiscent of a late-stage spliceosome
CC       composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC       cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC       cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC       cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC       cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC       prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC       smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10759889}.
CC   -!- SIMILARITY: Belongs to the U2 small nuclear ribonucleoprotein A family.
CC       {ECO:0000305}.
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DR   EMBL; CU329671; CAB52744.1; -; Genomic_DNA.
DR   EMBL; AB027844; BAA87148.1; -; Genomic_DNA.
DR   PIR; T39747; T39747.
DR   RefSeq; NP_596725.1; NM_001022650.2.
DR   PDB; 3JB9; EM; 3.60 A; j=1-239.
DR   PDBsum; 3JB9; -.
DR   AlphaFoldDB; Q9USX8; -.
DR   SMR; Q9USX8; -.
DR   BioGRID; 276282; 86.
DR   IntAct; Q9USX8; 6.
DR   STRING; 4896.SPBC1861.08c.1; -.
DR   iPTMnet; Q9USX8; -.
DR   MaxQB; Q9USX8; -.
DR   PaxDb; Q9USX8; -.
DR   PRIDE; Q9USX8; -.
DR   GeneID; 2539730; -.
DR   KEGG; spo:SPBC1861.08c; -.
DR   PomBase; SPBC1861.08c; lea1.
DR   eggNOG; KOG1644; Eukaryota.
DR   HOGENOM; CLU_061027_1_0_1; -.
DR   InParanoid; Q9USX8; -.
DR   PhylomeDB; Q9USX8; -.
DR   PRO; PR:Q9USX8; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR   GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR   GO; GO:0005686; C:U2 snRNP; IDA:PomBase.
DR   GO; GO:0071004; C:U2-type prespliceosome; ISO:PomBase.
DR   GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:PomBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR044640; RU2A.
DR   PANTHER; PTHR10552; PTHR10552; 1.
DR   PROSITE; PS51450; LRR; 3.
PE   1: Evidence at protein level;
KW   3D-structure; Leucine-rich repeat; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Repeat; Spliceosome.
FT   CHAIN           1..239
FT                   /note="U2 small nuclear ribonucleoprotein A'"
FT                   /id="PRO_0000074186"
FT   REPEAT          19..40
FT                   /note="LRR 1"
FT   REPEAT          42..63
FT                   /note="LRR 2"
FT   REPEAT          64..85
FT                   /note="LRR 3"
FT   REPEAT          88..109
FT                   /note="LRR 4"
FT   DOMAIN          122..160
FT                   /note="LRRCT"
SQ   SEQUENCE   239 AA;  27228 MW;  CAB29D7CE38D3938 CRC64;
     MRLNAEFLSQ VPSFISPLKE TELDLRWYQI PIIENLGVLR DVHDAIDFTD NDIRYLGNFP
     RMKRLQTLLC GNNRITAIAP DIGKVLPNLK TLSLAQNHLQ EIADLDPLAS CPQLTNLSCI
     DNPVAQKQYY RLYLIWRIPS LHILDFERVR RNERLRAEEV FGQIQNPTEI ASSIMGVKSR
     VFDLAALVQS HPEANSPITT GYTLTPEERE KIKEAIKNAS SIAEINRLEA MLLEGKIPK