RU2B2_ARATH
ID RU2B2_ARATH Reviewed; 229 AA.
AC Q8H1S6; A8MRK1; F4HNV4; Q8RXP9; Q9LMJ0;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=U2 small nuclear ribonucleoprotein B'' 2;
DE Short=U2 snRNP B'' 2;
GN OrderedLocusNames=At1g06960; ORFNames=F10K1.32;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=9819359; DOI=10.1242/jcs.111.24.3687;
RA Boudonck K., Dolan L., Shaw P.J.;
RT "Coiled body numbers in the Arabidopsis root epidermis are regulated by
RT cell type, developmental stage and cell cycle parameters.";
RL J. Cell Sci. 111:3687-3694(1998).
RN [5]
RP SUBUNIT, AND GENE FAMILY.
RX PubMed=15987817; DOI=10.1261/rna.2440305;
RA Lorkovic Z.J., Lehner R., Forstner C., Barta A.;
RT "Evolutionary conservation of minor U12-type spliceosome between plants and
RT humans.";
RL RNA 11:1095-1107(2005).
CC -!- FUNCTION: Involved in nuclear pre-mRNA splicing. {ECO:0000250}.
CC -!- SUBUNIT: Component of the spliceosome where it is associated with snRNP
CC U2. {ECO:0000269|PubMed:15987817}.
CC -!- SUBCELLULAR LOCATION: Nucleus, Cajal body {ECO:0000269|PubMed:9819359}.
CC Nucleus, nucleoplasm {ECO:0000269|PubMed:9819359}. Cytoplasm
CC {ECO:0000269|PubMed:9819359}. Note=Present in coiled bodies and an
CC interchromatin network. Redistributed throughout the cytoplasm upon
CC entry into mitosis. {ECO:0000269|PubMed:9819359}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8H1S6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8H1S6-2; Sequence=VSP_043003;
CC Name=3;
CC IsoId=Q8H1S6-3; Sequence=VSP_043002;
CC -!- SIMILARITY: Belongs to the RRM U1 A/B'' family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF82223.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC067971; AAF82223.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28058.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28060.1; -; Genomic_DNA.
DR EMBL; AY080742; AAL85989.1; -; mRNA.
DR EMBL; AY142487; AAN13038.1; -; mRNA.
DR PIR; C86204; C86204.
DR RefSeq; NP_001077472.1; NM_001084003.2. [Q8H1S6-3]
DR RefSeq; NP_850936.1; NM_180605.2. [Q8H1S6-1]
DR AlphaFoldDB; Q8H1S6; -.
DR SMR; Q8H1S6; -.
DR BioGRID; 22447; 16.
DR STRING; 3702.AT1G06960.1; -.
DR PaxDb; Q8H1S6; -.
DR PRIDE; Q8H1S6; -.
DR ProMEX; Q8H1S6; -.
DR ProteomicsDB; 228049; -. [Q8H1S6-1]
DR EnsemblPlants; AT1G06960.1; AT1G06960.1; AT1G06960. [Q8H1S6-1]
DR EnsemblPlants; AT1G06960.3; AT1G06960.3; AT1G06960. [Q8H1S6-3]
DR GeneID; 837206; -.
DR Gramene; AT1G06960.1; AT1G06960.1; AT1G06960. [Q8H1S6-1]
DR Gramene; AT1G06960.3; AT1G06960.3; AT1G06960. [Q8H1S6-3]
DR KEGG; ath:AT1G06960; -.
DR Araport; AT1G06960; -.
DR TAIR; locus:2007417; AT1G06960.
DR eggNOG; KOG4206; Eukaryota.
DR OMA; ADINPNH; -.
DR PhylomeDB; Q8H1S6; -.
DR PRO; PR:Q8H1S6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H1S6; baseline and differential.
DR Genevisible; Q8H1S6; AT.
DR GO; GO:0015030; C:Cajal body; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0005686; C:U2 snRNP; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009631; P:cold acclimation; IMP:TAIR.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..229
FT /note="U2 small nuclear ribonucleoprotein B'' 2"
FT /id="PRO_0000416930"
FT DOMAIN 10..89
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 155..229
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 104..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..123
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..36
FT /note="MLTADIPPNQSIYIKHINEKIKKEELKRSLYCLFSQ -> MRRSRKK (in
FT isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_043002"
FT VAR_SEQ 140
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_043003"
FT CONFLICT 18
FT /note="N -> S (in Ref. 3; AAL85989)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="Q -> R (in Ref. 3; AAL85989)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 229 AA; 26223 MW; 999F6549EDC3DE49 CRC64;
MLTADIPPNQ SIYIKHINEK IKKEELKRSL YCLFSQFGRL LDVVALKTPK LRGQAWVVFT
EVTAASNAVR QMQNFPFYDK PMRIQYAKSK SDYVTKAEGS FVPKEKKMKQ EEKVERKRHA
EETQQPSMPN GATTQNGMPV PPFQPSGQDT MPPNNILFIH NLPIETNSMM LQLLFEQYPG
FKEIRMIEAK PGIAFVEYED DVQSSMAMQA LQGFKITPQN PMVVSFAKK
