ID   RUB3_ARATH              Reviewed;          78 AA.
AC   O65381;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=NEDD8-like protein RUB3;
DE   AltName: Full=Ubiquitin-related protein 3;
DE            Short=AtRUB3;
DE   Flags: Precursor;
GN   Name=RUB3; Synonyms=UBQ16; OrderedLocusNames=At1g11980; ORFNames=F12F1.15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   TISSUE SPECIFICITY.
RX   PubMed=9857029; DOI=10.1074/jbc.273.52.34976;
RA   Rao-Naik C., delaCruz W., Laplaza J.M., Tan S., Callis J., Fisher A.J.;
RT   "The rub family of ubiquitin-like proteins. Crystal structure of
RT   Arabidopsis rub1 and expression of multiple rubs in Arabidopsis.";
RL   J. Biol. Chem. 273:34976-34982(1998).
CC   -!- FUNCTION: May function as a stable post-translational protein modifier.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Detected in stems and flower buds, but not in
CC       leaves, mature flowers and seedlings. {ECO:0000269|PubMed:9857029}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC002131; AAC17623.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE28823.1; -; Genomic_DNA.
DR   PIR; G86254; G86254.
DR   RefSeq; NP_172662.1; NM_101070.1.
DR   AlphaFoldDB; O65381; -.
DR   SMR; O65381; -.
DR   STRING; 3702.AT1G11980.1; -.
DR   PaxDb; O65381; -.
DR   EnsemblPlants; AT1G11980.1; AT1G11980.1; AT1G11980.
DR   GeneID; 837750; -.
DR   Gramene; AT1G11980.1; AT1G11980.1; AT1G11980.
DR   KEGG; ath:AT1G11980; -.
DR   Araport; AT1G11980; -.
DR   TAIR; locus:2008950; AT1G11980.
DR   eggNOG; KOG0005; Eukaryota.
DR   HOGENOM; CLU_010412_6_4_1; -.
DR   InParanoid; O65381; -.
DR   OMA; YAGKQMA; -.
DR   OrthoDB; 1536766at2759; -.
DR   PhylomeDB; O65381; -.
DR   PRO; PR:O65381; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; O65381; baseline.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IEA:UniProt.
DR   GO; GO:0031386; F:protein tag; IBA:GO_Central.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR   GO; GO:0019941; P:modification-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; IBA:GO_Central.
DR   GO; GO:0030162; P:regulation of proteolysis; IBA:GO_Central.
DR   CDD; cd01806; Ubl_NEDD8; 1.
DR   InterPro; IPR038738; Nedd8-like.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   InterPro; IPR019954; Ubiquitin_CS.
DR   InterPro; IPR019956; Ubiquitin_dom.
DR   Pfam; PF00240; ubiquitin; 1.
DR   PRINTS; PR00348; UBIQUITIN.
DR   SMART; SM00213; UBQ; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS00299; UBIQUITIN_1; 1.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
PE   2: Evidence at transcript level;
KW   Isopeptide bond; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..76
FT                   /note="NEDD8-like protein RUB3"
FT                   /id="PRO_0000035969"
FT   PROPEP          77..78
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000035970"
FT   CROSSLNK        76
FT                   /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT                   K-? in acceptor proteins)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
SQ   SEQUENCE   78 AA;  8940 MW;  E20603430CCAEAEC CRC64;
     MEIKVKTLTE KQIDIEIELT DTIERIKERI EEKEGIPPVH QRIVYTGKQL ADDLTAKHYN
     LERGSVLHLV LALRGGCC