ID   RUBA_WHEAT              Reviewed;         543 AA.
AC   P08823;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=RuBisCO large subunit-binding protein subunit alpha, chloroplastic;
DE   AltName: Full=60 kDa chaperonin subunit alpha;
DE   AltName: Full=CPN-60 alpha;
DE   Flags: Precursor; Fragment;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 3-22.
RC   TISSUE=Seed;
RX   PubMed=2897629; DOI=10.1038/333330a0;
RA   Hemmingsen S.M., Woolford C., van der Vies S.M., Tilly K., Dennis D.T.,
RA   Georgopoulos C., Hendrix R.W., Ellis R.J.;
RT   "Homologous plant and bacterial proteins chaperone oligomeric protein
RT   assembly.";
RL   Nature 333:330-334(1988).
CC   -!- FUNCTION: This protein binds RuBisCO small and large subunits and is
CC       implicated in the assembly of the enzyme oligomer.
CC   -!- SUBUNIT: Oligomer of probably six alpha and six beta subunits.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: This protein shows ATPase activity.
CC   -!- SIMILARITY: Belongs to the chaperonin (HSP60) family. {ECO:0000305}.
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DR   EMBL; X07851; CAA30699.1; -; mRNA.
DR   PIR; S02198; HHWTBA.
DR   AlphaFoldDB; P08823; -.
DR   SMR; P08823; -.
DR   STRING; 4565.Traes_2AS_C1EA81EC4.2; -.
DR   PRIDE; P08823; -.
DR   eggNOG; KOG0356; Eukaryota.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P08823; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR   GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR   CDD; cd03344; GroEL; 1.
DR   Gene3D; 1.10.560.10; -; 1.
DR   Gene3D; 3.30.260.10; -; 1.
DR   Gene3D; 3.50.7.10; -; 1.
DR   HAMAP; MF_00600; CH60; 1.
DR   InterPro; IPR018370; Chaperonin_Cpn60_CS.
DR   InterPro; IPR001844; Cpn60/GroEL.
DR   InterPro; IPR002423; Cpn60/GroEL/TCP-1.
DR   InterPro; IPR027409; GroEL-like_apical_dom_sf.
DR   InterPro; IPR027413; GROEL-like_equatorial_sf.
DR   InterPro; IPR027410; TCP-1-like_intermed_sf.
DR   Pfam; PF00118; Cpn60_TCP1; 1.
DR   PRINTS; PR00298; CHAPERONIN60.
DR   SUPFAM; SSF48592; SSF48592; 1.
DR   SUPFAM; SSF52029; SSF52029; 1.
DR   SUPFAM; SSF54849; SSF54849; 1.
DR   TIGRFAMs; TIGR02348; GroEL; 1.
DR   PROSITE; PS00296; CHAPERONINS_CPN60; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Chaperone; Chloroplast; Direct protein sequencing;
KW   Nucleotide-binding; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         <1..2
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|PubMed:2897629"
FT   CHAIN           3..543
FT                   /note="RuBisCO large subunit-binding protein subunit alpha,
FT                   chloroplastic"
FT                   /id="PRO_0000005021"
FT   CONFLICT        5
FT                   /note="K -> P (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        12
FT                   /note="K -> G (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
SQ   SEQUENCE   543 AA;  57521 MW;  28C925E77DAEF0EB CRC64;
     GADAKEIAFD QKSRAALQAG VEKLANAVGV TLGPRGRNVV LDEYGNPKVV NDGVTIARAI
     ELANPMENAG AALIREVASK TNDSAGDGTT TACVLAREII KLGILSVTSG ANPVSLKKGI
     DKTVQGLIEE LERKARPVKG SGDIKAVASI SAGNDELIGA MIADAIDKVG PDGVLSIESS
     SSFETTVDVE EGMEIDRGYI SPQFVTNLEK SIVEFENARV LITDQKITSI KEIIPLLEQT
     TQLRCPLFIV AEDITGEALA TLVVNKLRGI INVAAIKAPS FGERRKAVLQ DIAIVTGAEY
     LAKDLGLLVE NATVDQLGTA RKITIHQTTT TLIADAASKD EIQARVAQLK KELSETDSIY
     DSEKLAERIA KLSGGVAVIK VGATTETELE DRQLRIEDAK NATFAAIEEG IVPGGGAAYV
     HLSTYVPAIK ETIEDHDERL GADIIQKALQ APASLIANNA GVEGEVVIEK IKESEWEMGY
     NAMTDKYENL IESGVIDPAK VTRCALQNAA SVSGMVLTTQ AIVVEKPKPK PKVAEPAEGQ
     LSV