RUBB_BRANA
ID RUBB_BRANA Reviewed; 588 AA.
AC P21241;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=RuBisCO large subunit-binding protein subunit beta, chloroplastic;
DE AltName: Full=60 kDa chaperonin subunit beta;
DE AltName: Full=CPN-60 beta;
DE Flags: Precursor;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Westar;
RX PubMed=1979547; DOI=10.1016/0378-1119(90)90385-5;
RA Martel R., Cloney L.P., Pelcher L.E., Hemmingsen S.M.;
RT "Unique composition of plastid chaperonin-60: alpha and beta polypeptide-
RT encoding genes are highly divergent.";
RL Gene 94:181-187(1990).
CC -!- FUNCTION: This protein binds RuBisCO small and large subunits and is
CC implicated in the assembly of the enzyme oligomer.
CC -!- SUBUNIT: Oligomer of probably six alpha and six beta subunits.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- MISCELLANEOUS: This protein shows ATPase activity.
CC -!- SIMILARITY: Belongs to the chaperonin (HSP60) family. {ECO:0000305}.
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DR EMBL; M35600; AAA32980.1; -; mRNA.
DR PIR; PW0007; PW0007.
DR AlphaFoldDB; P21241; -.
DR SMR; P21241; -.
DR PRIDE; P21241; -.
DR EnsemblPlants; CDY02438; CDY02438; GSBRNA2T00114056001.
DR Gramene; CDY02438; CDY02438; GSBRNA2T00114056001.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR CDD; cd03344; GroEL; 1.
DR Gene3D; 1.10.560.10; -; 1.
DR Gene3D; 3.30.260.10; -; 1.
DR Gene3D; 3.50.7.10; -; 1.
DR HAMAP; MF_00600; CH60; 1.
DR InterPro; IPR018370; Chaperonin_Cpn60_CS.
DR InterPro; IPR001844; Cpn60/GroEL.
DR InterPro; IPR002423; Cpn60/GroEL/TCP-1.
DR InterPro; IPR027409; GroEL-like_apical_dom_sf.
DR InterPro; IPR027413; GROEL-like_equatorial_sf.
DR InterPro; IPR027410; TCP-1-like_intermed_sf.
DR Pfam; PF00118; Cpn60_TCP1; 1.
DR PRINTS; PR00298; CHAPERONIN60.
DR SUPFAM; SSF48592; SSF48592; 1.
DR SUPFAM; SSF52029; SSF52029; 1.
DR SUPFAM; SSF54849; SSF54849; 1.
DR TIGRFAMs; TIGR02348; GroEL; 1.
DR PROSITE; PS00296; CHAPERONINS_CPN60; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chaperone; Chloroplast; Nucleotide-binding; Phosphoprotein;
KW Plastid; Transit peptide.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT CHAIN 56..588
FT /note="RuBisCO large subunit-binding protein subunit beta,
FT chloroplastic"
FT /id="PRO_0000005023"
FT MOD_RES 101
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P21238"
FT MOD_RES 478
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P21240"
SQ SEQUENCE 588 AA; 62473 MW; EFE721105C436B3F CRC64;
MASTFTATSS IGSMVAPNAH KTDKKLMNKL SSSSFGRRQN VFPKLRRSSP AVVCAAKELH
FNKDGTTIRK LQAGVNKLAD LVGVTLGPKG RNVVLESKYG SPRIVNDGVT VAREVELEDP
VENIGAKLVR QAAAKTNDLA GDGTTTSVVL AQGFIAEGVK VVAAGANPVL ITRGIEKTAK
ALVAELKKMS KEVEDSELAD VAAVSAGNNA EIGSMIAEAM SRVGRKGVVT LEEGKSAENA
LYVVEGMQFD RGYVSPYFVT DSEKMSVEFD NCKLLLVDKK ITNARDLVGV LEDAIRGGYP
ILIIAEDIEQ EALATLVVNK LRGTLKIAAL KAPGFGERKS QYLDDIAILT GATVIREEVG
LSLDKAGKEV LGHAAKVVLT KETSTIVGDG STQDAVQKRV TQIKNLIEQA EQDYEKEKLN
ERIAKLSGGV AVIQVGAQTE TELKEKKLRV EDALNATKAA VEEGIVVGGG CTLLRLASKV
DAIKAALDND EEKVGADIVK RALSYPLKLI AKNAGVNGSV VSEKVLSNDN VKFGYNAATG
KYEDLMAAGI IDPTKVVRCC LEHAASVAKT FLMSDCVVVE IKEPEPVP
