ID   RUG1_LIMKU              Reviewed;          75 AA.
AC   C3RT23;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Rugosin-LK1 {ECO:0000303|PubMed:23054029};
DE   Flags: Precursor;
OS   Limnonectes kuhlii (Kuhl's Creek frog) (Rana kuhlii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Dicroglossidae; Dicroglossinae;
OC   Limnonectes.
OX   NCBI_TaxID=110107;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-75, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000303|PubMed:23054029}, and
RC   Skin secretion {ECO:0000303|PubMed:23054029};
RX   PubMed=23054029; DOI=10.1007/s11033-012-2152-4;
RA   Wang G., Wang Y., Ma D., Liu H., Li J., Zhang K., Yang X., Lai R., Liu J.;
RT   "Five novel antimicrobial peptides from the Kuhl's wart frog skin
RT   secretions, Limnonectes kuhlii.";
RL   Mol. Biol. Rep. 40:1097-1102(2013).
CC   -!- FUNCTION: Has antimicrobial activity against Gram-positive bacteria
CC       S.aureus ATCC 2592 (MIC=10.0 uM), S.aureus ATCC 43300 (MIC=15.0 uM) and
CC       B.subtilis (MIC=40.0 uM), against Gram-negative bacteria E.coli ML-35P
CC       (MIC=10.0 uM), P.aeruginosa PA01 (MIC=5.0 uM) and P.aeruginosa ATCC
CC       27853 (MIC=5.0 uM) and against fungus C.albicans ATCC 2002 (MIC=10.0
CC       uM). {ECO:0000269|PubMed:23054029}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255,
CC       ECO:0000269|PubMed:23054029}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:23054029}.
CC   -!- MASS SPECTROMETRY: Mass=3520.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23054029};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000255}.
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DR   EMBL; EU346906; ACB12322.1; -; mRNA.
DR   AlphaFoldDB; C3RT23; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF08023; Antimicrobial_2; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Direct protein sequencing; Fungicide;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..40
FT                   /evidence="ECO:0000305|PubMed:23054029"
FT                   /id="PRO_0000445403"
FT   PEPTIDE         43..75
FT                   /note="Rugosin-LK1"
FT                   /evidence="ECO:0000269|PubMed:23054029"
FT                   /id="PRO_0000445404"
SQ   SEQUENCE   75 AA;  8475 MW;  A5FC66C931769407 CRC64;
     MFTMKKSLLF LFFLGTISLS FCEEERSADE DDEGEMTEEE KRSIRDKIKT MAIDLAKSAG
     TGVLKTLICK LDKSC