RUN3A_HUMAN
ID RUN3A_HUMAN Reviewed; 446 AA.
AC Q59EK9; B2R974; O15483; O60651; Q7Z3S2; Q9UF50;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=RUN domain-containing protein 3A;
DE AltName: Full=Rap2-interacting protein 8;
DE Short=RPIP-8;
GN Name=RUNDC3A; Synonyms=RAP2IP, RPIP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND IDENTIFICATION (ISOFORM 1).
RX PubMed=9523700; DOI=10.1046/j.1432-1327.1998.2520290.x;
RA Janoueix-Lerosey I., Pasheva E., de Tand M.-F., Tavitian A.,
RA de Gunzburg J.;
RT "Identification of a specific effector of the small GTP-binding protein
RT Rap2.";
RL Eur. J. Biochem. 252:290-298(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RA Yu W., Gibbs R.A.;
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Caudate nucleus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.,
RA Ohara O., Nagase T., Kikuno R.F.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
RC TISSUE=Cerebellum, and Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May act as an effector of RAP2A in neuronal cells.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the GTP-bound form of RAP2A. {ECO:0000250}.
CC -!- INTERACTION:
CC Q59EK9; Q9BXS5: AP1M1; NbExp=4; IntAct=EBI-747225, EBI-541426;
CC Q59EK9; Q9H6L4: ARMC7; NbExp=3; IntAct=EBI-747225, EBI-742909;
CC Q59EK9; Q96S94: CCNL2; NbExp=3; IntAct=EBI-747225, EBI-1045974;
CC Q59EK9; P26196: DDX6; NbExp=3; IntAct=EBI-747225, EBI-351257;
CC Q59EK9; P60228: EIF3E; NbExp=4; IntAct=EBI-747225, EBI-347740;
CC Q59EK9; Q3B820: FAM161A; NbExp=3; IntAct=EBI-747225, EBI-719941;
CC Q59EK9; P55040: GEM; NbExp=3; IntAct=EBI-747225, EBI-744104;
CC Q59EK9; O15481: MAGEB4; NbExp=3; IntAct=EBI-747225, EBI-751857;
CC Q59EK9; Q96A72: MAGOHB; NbExp=3; IntAct=EBI-747225, EBI-746778;
CC Q59EK9; Q9P015: MRPL15; NbExp=3; IntAct=EBI-747225, EBI-2371967;
CC Q59EK9; P61225: RAP2B; NbExp=4; IntAct=EBI-747225, EBI-750871;
CC Q59EK9; O00560: SDCBP; NbExp=3; IntAct=EBI-747225, EBI-727004;
CC Q59EK9; Q9BSW7: SYT17; NbExp=3; IntAct=EBI-747225, EBI-745392;
CC Q59EK9; Q05BL0: TBRG1; NbExp=3; IntAct=EBI-747225, EBI-10223693;
CC Q59EK9; P0C1Z6-2: TFPT; NbExp=3; IntAct=EBI-747225, EBI-10178002;
CC Q59EK9; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-747225, EBI-740727;
CC Q59EK9; Q96SQ5: ZNF587; NbExp=3; IntAct=EBI-747225, EBI-6427977;
CC Q59EK9; Q9H614; NbExp=3; IntAct=EBI-747225, EBI-10249899;
CC Q59EK9-3; Q9BXS5: AP1M1; NbExp=3; IntAct=EBI-11957366, EBI-541426;
CC Q59EK9-3; Q9H6L4: ARMC7; NbExp=3; IntAct=EBI-11957366, EBI-742909;
CC Q59EK9-3; Q96S94-5: CCNL2; NbExp=3; IntAct=EBI-11957366, EBI-12024864;
CC Q59EK9-3; P60228: EIF3E; NbExp=3; IntAct=EBI-11957366, EBI-347740;
CC Q59EK9-3; Q9H0I2: ENKD1; NbExp=3; IntAct=EBI-11957366, EBI-744099;
CC Q59EK9-3; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-11957366, EBI-739832;
CC Q59EK9-3; O15481: MAGEB4; NbExp=3; IntAct=EBI-11957366, EBI-751857;
CC Q59EK9-3; Q8NHZ7: MBD3L2; NbExp=3; IntAct=EBI-11957366, EBI-11989378;
CC Q59EK9-3; Q8WWY3: PRPF31; NbExp=3; IntAct=EBI-11957366, EBI-1567797;
CC Q59EK9-3; P10114: RAP2A; NbExp=3; IntAct=EBI-11957366, EBI-602366;
CC Q59EK9-3; P61225: RAP2B; NbExp=3; IntAct=EBI-11957366, EBI-750871;
CC Q59EK9-3; O00560: SDCBP; NbExp=3; IntAct=EBI-11957366, EBI-727004;
CC Q59EK9-3; A0A0S2Z4F2: TEAD4; NbExp=3; IntAct=EBI-11957366, EBI-16429215;
CC Q59EK9-3; Q15561: TEAD4; NbExp=3; IntAct=EBI-11957366, EBI-747736;
CC Q59EK9-3; Q3SY00: TSGA10IP; NbExp=3; IntAct=EBI-11957366, EBI-10241197;
CC Q59EK9-3; P0CB47: UBTFL1; NbExp=3; IntAct=EBI-11957366, EBI-17208936;
CC Q59EK9-3; O75604: USP2; NbExp=3; IntAct=EBI-11957366, EBI-743272;
CC Q59EK9-3; P15622-3: ZNF250; NbExp=3; IntAct=EBI-11957366, EBI-10177272;
CC Q59EK9-3; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-11957366, EBI-740727;
CC Q59EK9-3; Q96SQ5: ZNF587; NbExp=3; IntAct=EBI-11957366, EBI-6427977;
CC Q59EK9-3; O75541-2: ZNF821; NbExp=3; IntAct=EBI-11957366, EBI-14544035;
CC Q59EK9-3; Q9Y2P0: ZNF835; NbExp=3; IntAct=EBI-11957366, EBI-5667516;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q59EK9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q59EK9-2; Sequence=VSP_032153, VSP_032155, VSP_032156;
CC Name=3;
CC IsoId=Q59EK9-3; Sequence=VSP_032155, VSP_032156;
CC Name=4;
CC IsoId=Q59EK9-4; Sequence=VSP_032154;
CC -!- SIMILARITY: Belongs to the RUNDC3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD93039.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U93871; AAB68767.1; -; mRNA.
DR EMBL; AF055026; AAC09366.1; -; mRNA.
DR EMBL; AK313669; BAG36421.1; -; mRNA.
DR EMBL; CR456748; CAG33029.1; -; mRNA.
DR EMBL; AB209802; BAD93039.1; ALT_INIT; mRNA.
DR EMBL; BX537443; CAD97685.1; -; mRNA.
DR EMBL; AL133657; CAB63771.1; -; mRNA.
DR EMBL; CH471178; EAW51609.1; -; Genomic_DNA.
DR EMBL; CH471178; EAW51610.1; -; Genomic_DNA.
DR EMBL; BC006194; AAH06194.1; -; mRNA.
DR CCDS; CCDS45698.1; -. [Q59EK9-1]
DR CCDS; CCDS45699.1; -. [Q59EK9-3]
DR CCDS; CCDS59294.1; -. [Q59EK9-2]
DR RefSeq; NP_001138297.1; NM_001144825.1. [Q59EK9-1]
DR RefSeq; NP_001138298.1; NM_001144826.1. [Q59EK9-2]
DR RefSeq; NP_006686.1; NM_006695.4. [Q59EK9-3]
DR AlphaFoldDB; Q59EK9; -.
DR SMR; Q59EK9; -.
DR BioGRID; 116106; 61.
DR IntAct; Q59EK9; 57.
DR MINT; Q59EK9; -.
DR STRING; 9606.ENSP00000410862; -.
DR iPTMnet; Q59EK9; -.
DR PhosphoSitePlus; Q59EK9; -.
DR BioMuta; RUNDC3A; -.
DR DMDM; 172045944; -.
DR jPOST; Q59EK9; -.
DR MassIVE; Q59EK9; -.
DR PaxDb; Q59EK9; -.
DR PeptideAtlas; Q59EK9; -.
DR PRIDE; Q59EK9; -.
DR ProteomicsDB; 62634; -. [Q59EK9-1]
DR ProteomicsDB; 62635; -. [Q59EK9-2]
DR ProteomicsDB; 62636; -. [Q59EK9-3]
DR ProteomicsDB; 62637; -. [Q59EK9-4]
DR Antibodypedia; 8218; 220 antibodies from 24 providers.
DR DNASU; 10900; -.
DR Ensembl; ENST00000225441.11; ENSP00000225441.7; ENSG00000108309.14. [Q59EK9-3]
DR Ensembl; ENST00000426726.8; ENSP00000410862.2; ENSG00000108309.14. [Q59EK9-1]
DR Ensembl; ENST00000590941.5; ENSP00000468214.1; ENSG00000108309.14. [Q59EK9-2]
DR GeneID; 10900; -.
DR KEGG; hsa:10900; -.
DR MANE-Select; ENST00000426726.8; ENSP00000410862.2; NM_001144825.2; NP_001138297.1.
DR UCSC; uc002igi.4; human. [Q59EK9-1]
DR CTD; 10900; -.
DR DisGeNET; 10900; -.
DR GeneCards; RUNDC3A; -.
DR HGNC; HGNC:16984; RUNDC3A.
DR HPA; ENSG00000108309; Tissue enhanced (brain, choroid plexus, pituitary gland).
DR MIM; 605448; gene.
DR neXtProt; NX_Q59EK9; -.
DR OpenTargets; ENSG00000108309; -.
DR PharmGKB; PA162402277; -.
DR VEuPathDB; HostDB:ENSG00000108309; -.
DR eggNOG; KOG4381; Eukaryota.
DR GeneTree; ENSGT00940000158922; -.
DR HOGENOM; CLU_045987_0_0_1; -.
DR InParanoid; Q59EK9; -.
DR OMA; MSEYIAT; -.
DR OrthoDB; 714315at2759; -.
DR PhylomeDB; Q59EK9; -.
DR TreeFam; TF323904; -.
DR PathwayCommons; Q59EK9; -.
DR SignaLink; Q59EK9; -.
DR BioGRID-ORCS; 10900; 15 hits in 1059 CRISPR screens.
DR ChiTaRS; RUNDC3A; human.
DR GeneWiki; RUNDC3A; -.
DR GenomeRNAi; 10900; -.
DR Pharos; Q59EK9; Tdark.
DR PRO; PR:Q59EK9; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q59EK9; protein.
DR Bgee; ENSG00000108309; Expressed in right frontal lobe and 160 other tissues.
DR Genevisible; Q59EK9; HS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0030695; F:GTPase regulator activity; TAS:ProtInc.
DR GO; GO:0010753; P:positive regulation of cGMP-mediated signaling; IBA:GO_Central.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; TAS:ProtInc.
DR Gene3D; 1.20.58.900; -; 1.
DR InterPro; IPR004012; Run_dom.
DR InterPro; IPR037213; Run_dom_sf.
DR Pfam; PF02759; RUN; 1.
DR SMART; SM00593; RUN; 1.
DR SUPFAM; SSF140741; SSF140741; 1.
DR PROSITE; PS50826; RUN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..446
FT /note="RUN domain-containing protein 3A"
FT /id="PRO_0000324155"
FT DOMAIN 52..189
FT /note="RUN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT REGION 1..298
FT /note="Interaction with RAP2A"
FT /evidence="ECO:0000250"
FT REGION 216..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 372..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 267..322
FT /evidence="ECO:0000255"
FT MOD_RES 215
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O08576"
FT MOD_RES 232
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O08576"
FT MOD_RES 416
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O08576"
FT MOD_RES 419
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O08576"
FT VAR_SEQ 75..79
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005,
FT ECO:0000303|PubMed:9523700"
FT /id="VSP_032153"
FT VAR_SEQ 365..446
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_032154"
FT VAR_SEQ 401..405
FT /note="KDPTP -> SSEPN (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005,
FT ECO:0000303|PubMed:9523700, ECO:0000303|Ref.2,
FT ECO:0000303|Ref.4"
FT /id="VSP_032155"
FT VAR_SEQ 406..446
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005,
FT ECO:0000303|PubMed:9523700, ECO:0000303|Ref.2,
FT ECO:0000303|Ref.4"
FT /id="VSP_032156"
FT CONFLICT 134
FT /note="M -> V (in Ref. 6; CAD97685)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 446 AA; 49747 MW; 2C753EA8619B1024 CRC64;
MEASFVQTTM ALGLSSKKAS SRNVAVERKN LITVCRFSVK TLLEKYTAEP IDDSSEEFVN
FAAILEQILS HRFKACAPAG PVSWFSSDGQ RGFWDYIRLA CSKVPNNCVS SIENMENIST
ARAKGRAWIR VALMEKRMSE YITTALRDTR TTRRFYDSGA IMLRDEATIL TGMLIGLSAI
DFSFCLKGEV LDGKTPVVID YTPYLKFTQS YDYLTDEEER HSAESSTSED NSPEHPYLPL
VTDEDSWYSK WHKMEQKFRI VYAQKGYLEE LVRLRESQLK DLEAENRRLQ LQLEEAAAQN
QREKRELEGV ILELQEQLTG LIPSDHAPLA QGSKELTTPL VNQWPSLGTL NGAEGASNSK
LYRRHSFMST EPLSAEASLS SDSQRLGEGT RDEEPWGPIG KDPTPSMLGL CGSLASIPSC
KSLASFKSNE CLVSDSPEGS PALSPS
