RUN3A_MOUSE
ID RUN3A_MOUSE Reviewed; 446 AA.
AC O08576; A2A693; A2A694; Q80Y95;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=RUN domain-containing protein 3A;
DE AltName: Full=Rap2-interacting protein 8;
DE Short=RPIP-8;
GN Name=Rundc3a; Synonyms=Rap2ip, Rpip8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION OF ISOFORM 2,
RP FUNCTION, INTERACTION WITH RAP2A, AND TISSUE SPECIFICITY.
RX PubMed=9523700; DOI=10.1046/j.1432-1327.1998.2520290.x;
RA Janoueix-Lerosey I., Pasheva E., de Tand M.-F., Tavitian A.,
RA de Gunzburg J.;
RT "Identification of a specific effector of the small GTP-binding protein
RT Rap2.";
RL Eur. J. Biochem. 252:290-298(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING
RP (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-215; SER-232; SER-416 AND
RP SER-419, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as an effector of RAP2A in neuronal cells.
CC {ECO:0000269|PubMed:9523700}.
CC -!- SUBUNIT: Interacts with the GTP-bound form of RAP2A.
CC {ECO:0000269|PubMed:9523700}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=O08576-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O08576-2; Sequence=VSP_032157, VSP_032159, VSP_032160;
CC Name=3;
CC IsoId=O08576-3; Sequence=VSP_032159, VSP_032160;
CC Name=4;
CC IsoId=O08576-4; Sequence=VSP_032158, VSP_032159, VSP_032160;
CC -!- TISSUE SPECIFICITY: Brain. {ECO:0000269|PubMed:9523700}.
CC -!- SIMILARITY: Belongs to the RUNDC3 family. {ECO:0000305}.
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DR EMBL; U73941; AAB51123.1; -; mRNA.
DR EMBL; AL596258; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC046319; AAH46319.1; -; mRNA.
DR CCDS; CCDS25497.1; -. [O08576-1]
DR CCDS; CCDS56812.1; -. [O08576-4]
DR RefSeq; NP_001239276.1; NM_001252347.1. [O08576-4]
DR RefSeq; NP_058039.1; NM_016759.4. [O08576-1]
DR RefSeq; XP_006533756.1; XM_006533693.2. [O08576-3]
DR RefSeq; XP_006533757.1; XM_006533694.2. [O08576-2]
DR AlphaFoldDB; O08576; -.
DR SMR; O08576; -.
DR BioGRID; 206182; 1.
DR STRING; 10090.ENSMUSP00000006750; -.
DR iPTMnet; O08576; -.
DR PhosphoSitePlus; O08576; -.
DR SwissPalm; O08576; -.
DR MaxQB; O08576; -.
DR PaxDb; O08576; -.
DR PeptideAtlas; O08576; -.
DR PRIDE; O08576; -.
DR ProteomicsDB; 256643; -. [O08576-1]
DR ProteomicsDB; 256644; -. [O08576-2]
DR ProteomicsDB; 256645; -. [O08576-3]
DR ProteomicsDB; 256646; -. [O08576-4]
DR Antibodypedia; 8218; 220 antibodies from 24 providers.
DR DNASU; 51799; -.
DR Ensembl; ENSMUST00000006750; ENSMUSP00000006750; ENSMUSG00000006575. [O08576-1]
DR Ensembl; ENSMUST00000107102; ENSMUSP00000102719; ENSMUSG00000006575. [O08576-3]
DR Ensembl; ENSMUST00000107103; ENSMUSP00000102720; ENSMUSG00000006575. [O08576-2]
DR Ensembl; ENSMUST00000107105; ENSMUSP00000102722; ENSMUSG00000006575. [O08576-4]
DR GeneID; 51799; -.
DR KEGG; mmu:51799; -.
DR UCSC; uc007lrq.3; mouse. [O08576-4]
DR UCSC; uc007lrr.3; mouse. [O08576-1]
DR CTD; 10900; -.
DR MGI; MGI:1858752; Rundc3a.
DR VEuPathDB; HostDB:ENSMUSG00000006575; -.
DR eggNOG; KOG4381; Eukaryota.
DR GeneTree; ENSGT00940000158922; -.
DR HOGENOM; CLU_045987_0_0_1; -.
DR InParanoid; O08576; -.
DR OMA; MSEYIAT; -.
DR OrthoDB; 714315at2759; -.
DR PhylomeDB; O08576; -.
DR TreeFam; TF323904; -.
DR BioGRID-ORCS; 51799; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Rundc3a; mouse.
DR PRO; PR:O08576; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; O08576; protein.
DR Bgee; ENSMUSG00000006575; Expressed in retinal neural layer and 161 other tissues.
DR Genevisible; O08576; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0010753; P:positive regulation of cGMP-mediated signaling; ISO:MGI.
DR Gene3D; 1.20.58.900; -; 1.
DR InterPro; IPR004012; Run_dom.
DR InterPro; IPR037213; Run_dom_sf.
DR Pfam; PF02759; RUN; 1.
DR SMART; SM00593; RUN; 1.
DR SUPFAM; SSF140741; SSF140741; 1.
DR PROSITE; PS50826; RUN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..446
FT /note="RUN domain-containing protein 3A"
FT /id="PRO_0000324156"
FT DOMAIN 52..189
FT /note="RUN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT REGION 1..298
FT /note="Interaction with RAP2A"
FT /evidence="ECO:0000269|PubMed:9523700"
FT REGION 216..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 372..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 267..322
FT /evidence="ECO:0000255"
FT MOD_RES 215
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 232
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 416
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 419
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 75..79
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_032157"
FT VAR_SEQ 318
FT /note="L -> LPDPPPPPR (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032158"
FT VAR_SEQ 401..405
FT /note="KDPTP -> SSEPN (in isoform 2, isoform 3 and isoform
FT 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032159"
FT VAR_SEQ 406..446
FT /note="Missing (in isoform 2, isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032160"
SQ SEQUENCE 446 AA; 50018 MW; 5D9D73127A216483 CRC64;
MEASFVQTTM ALGLPSKKAS SRNVIVERRN LITVCRFSVK TLLEKYTAEP IDDSSEEFVN
FAAILEQILS HRFKACAPAG PASWFSSDGQ RGFWDYIRLA CSKVPNNCVS SIENMENIST
ARAKGRAWIR VALMEKRMSE YITTALRDNR TTRRFYDSGA IMLREEATVL TGMLIGLSAI
DFSFCLKGEV LDGKTPVVID YTPYLKFTQS YDYLTDEEER HSAESSTSED NSPEHPYLPL
VTDEDSWYNK WHKMEQKFRI VYAQKGYLEE LVRLRESQLK DLEAENRRLQ LQLEEAAAQN
QREKRELEGV ILELQEQLTG LIPGDHAPLA QGSKELTTSL VNQWPSLSTL HRPEGASNSK
LYRRHSFMST EPLSAEASLS SDSQRLGEAK RDEEPWGPIG KDPTPSMLGL CGSLASIPSC
KSLASFKSNE CLVSDSPEGS PALSPS
