ID   RUNX2_HORSE             Reviewed;         131 AA.
AC   Q9XSB7;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Runt-related transcription factor 2;
DE   AltName: Full=Core-binding factor subunit alpha-1;
DE            Short=CBF-alpha-1;
DE   Flags: Fragment;
GN   Name=RUNX2; Synonyms=CBFA1;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Uemura T., Fu Z.W., Kato H.;
RT   "Molecular cloning and expression of equine Cbfa1.";
RL   Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor involved in osteoblastic differentiation
CC       and skeletal morphogenesis. Essential for the maturation of osteoblasts
CC       and both intramembranous and endochondral ossification. CBF binds to
CC       the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters,
CC       including murine leukemia virus, polyomavirus enhancer, T-cell receptor
CC       enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I)
CC       collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent
CC       transcriptional activation. In osteoblasts, supports transcription
CC       activation: synergizes with SPEN/MINT to enhance FGFR2-mediated
CC       activation of the osteocalcin FGF-responsive element (OCFRE).
CC       {ECO:0000250|UniProtKB:Q13950}.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit
CC       binds DNA as a monomer and through the Runt domain. DNA-binding is
CC       increased by heterodimerization (By similarity). Interacts with XRCC6
CC       (Ku70) and XRCC5 (Ku80). Interacts with CCNB1, KAT6A and KAT6B.
CC       Interacts with HIVEP3. Interacts with IFI204. Interaction with SATB2;
CC       the interaction results in enhanced DNA binding and transactivation by
CC       these transcription factors. Binds to HIPK3. Interacts (isoform 3) with
CC       DDX5. Interacts with FOXO1 (via a C-terminal region); the interaction
CC       inhibits RUNX2 transcriptional activity towards BGLAP. This interaction
CC       is prevented on insulin or IGF1 stimulation as FOXO1 is exported from
CC       the nucleus. Interacts with FOXP3. Interacts with TMEM119. Interacts
CC       with OLFM2 (By similarity). {ECO:0000250|UniProtKB:Q08775,
CC       ECO:0000250|UniProtKB:Q13950, ECO:0000250|UniProtKB:Q9Z2J9}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13950}.
CC   -!- PTM: Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by
CC       HIPK3 is required for the SPEN/MINT and FGF2 transactivation during
CC       osteoblastic differentiation (By similarity). {ECO:0000250}.
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DR   EMBL; AF113507; AAD26154.1; -; mRNA.
DR   AlphaFoldDB; Q9XSB7; -.
DR   PaxDb; Q9XSB7; -.
DR   InParanoid; Q9XSB7; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0002062; P:chondrocyte differentiation; IBA:GO_Central.
DR   GO; GO:0030097; P:hemopoiesis; IBA:GO_Central.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0001503; P:ossification; IBA:GO_Central.
DR   GO; GO:0045669; P:positive regulation of osteoblast differentiation; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045595; P:regulation of cell differentiation; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 4.10.770.10; -; 1.
DR   InterPro; IPR000040; AML1_Runt.
DR   InterPro; IPR027384; Runx_central_dom_sf.
DR   PANTHER; PTHR11950; PTHR11950; 1.
PE   2: Evidence at transcript level;
KW   Differentiation; DNA-binding; Methylation; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           <1..>131
FT                   /note="Runt-related transcription factor 2"
FT                   /id="PRO_0000174658"
FT   DOMAIN          <1..10
FT                   /note="Runt"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00399"
FT   REGION          1..75
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..73
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         11
FT                   /note="Asymmetric dimethylarginine"
FT                   /evidence="ECO:0000250|UniProtKB:Q08775"
FT   NON_TER         1
FT   NON_TER         131
SQ   SEQUENCE   131 AA;  14249 MW;  2516955230654247 CRC64;
     MRVGVPPQIP RPSLNSAPSP FNPQGQSQIT DPRQAQSPPP WSYDQSYPSY LSQMTSPSIH
     STTPLSSTRG TGLPVITDVP RRISGASELG PFSDPRQFPS ISSLTESRFS NPRMHYPATF
     TYTPPVTSGM S