RUNX2_RAT
ID RUNX2_RAT Reviewed; 218 AA.
AC Q9Z2J9; Q99NC7;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Runt-related transcription factor 2;
DE AltName: Full=Core-binding factor subunit alpha-1;
DE Short=CBF-alpha-1;
DE AltName: Full=Osteoblast-specific transcription factor 2;
DE Short=OSF-2;
DE Flags: Fragments;
GN Name=Runx2; Synonyms=Cbfa1, Osf2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98.
RX PubMed=9651525; DOI=10.1016/s0378-1119(98)00227-3;
RA Xiao Z.S., Thomas R., Hinson T.K., Quarles L.D.;
RT "Genomic structure and isoform expression of the mouse, rat and human
RT Cbfa1/Osf2 transcription factor.";
RL Gene 214:187-197(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 99-218.
RC TISSUE=Osteoblast;
RA Goseki M.S., Iimura T.;
RT "Regulatory expression of rat CBFA1 in osteoblastic cells.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP INTERACTION WITH OLFM2.
RX PubMed=28062493; DOI=10.1161/atvbaha.116.308606;
RA Shi N., Li C.X., Cui X.B., Tomarev S.I., Chen S.Y.;
RT "Olfactomedin 2 regulates smooth muscle phenotypic modulation and vascular
RT remodeling through mediating Runt-related transcription factor 2 binding to
RT serum response factor.";
RL Arterioscler. Thromb. Vasc. Biol. 37:446-454(2017).
CC -!- FUNCTION: Transcription factor involved in osteoblastic differentiation
CC and skeletal morphogenesis. Essential for the maturation of osteoblasts
CC and both intramembranous and endochondral ossification. CBF binds to
CC the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters,
CC including murine leukemia virus, polyomavirus enhancer, T-cell receptor
CC enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I)
CC collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent
CC transcriptional activation. In osteoblasts, supports transcription
CC activation: synergizes with SPEN/MINT to enhance FGFR2-mediated
CC activation of the osteocalcin FGF-responsive element (OCFRE).
CC {ECO:0000250|UniProtKB:Q13950}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit
CC binds DNA as a monomer and through the Runt domain. DNA-binding is
CC increased by heterodimerization (By similarity). Interacts with XRCC6
CC (Ku70) and XRCC5 (Ku80). Interacts with CCNB1, KAT6A and KAT6B.
CC Interacts with HIVEP3. Interacts with IFI204. Interaction with SATB2;
CC the interaction results in enhanced DNA binding and transactivation by
CC these transcription factors. Binds to HIPK3. Interacts (isoform 3) with
CC DDX5. Interacts with FOXO1 (via a C-terminal region); the interaction
CC inhibits RUNX2 transcriptional activity towards BGLAP. Interacts with
CC FOXP3. Interacts with TMEM119. Interacts with OLFM2 (PubMed:28062493).
CC {ECO:0000250|UniProtKB:Q08775, ECO:0000250|UniProtKB:Q13950,
CC ECO:0000269|PubMed:28062493}.
CC -!- INTERACTION:
CC Q9Z2J9; P25977: Ubtf; NbExp=2; IntAct=EBI-6119952, EBI-15620127;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13950}.
CC -!- PTM: Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by
CC HIPK3 is required for the SPEN/MINT and FGF2 transactivation during
CC osteoblastic differentiation (By similarity). {ECO:0000250}.
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DR EMBL; AF053950; AAC77442.1; -; Genomic_DNA.
DR EMBL; AB025797; BAB40441.1; -; mRNA.
DR AlphaFoldDB; Q9Z2J9; -.
DR BMRB; Q9Z2J9; -.
DR SMR; Q9Z2J9; -.
DR CORUM; Q9Z2J9; -.
DR DIP; DIP-60266N; -.
DR IntAct; Q9Z2J9; 2.
DR STRING; 10116.ENSRNOP00000057733; -.
DR PaxDb; Q9Z2J9; -.
DR UCSC; RGD:2282; rat.
DR RGD; 2282; Runx2.
DR eggNOG; KOG3982; Eukaryota.
DR InParanoid; Q9Z2J9; -.
DR PhylomeDB; Q9Z2J9; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0000785; C:chromatin; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0043425; F:bHLH transcription factor binding; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0031490; F:chromatin DNA binding; ISO:RGD.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:RGD.
DR GO; GO:0140296; F:general transcription initiation factor binding; IPI:RGD.
DR GO; GO:0042826; F:histone deacetylase binding; IPI:RGD.
DR GO; GO:0019904; F:protein domain specific binding; ISO:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:RGD.
DR GO; GO:0030509; P:BMP signaling pathway; ISO:RGD.
DR GO; GO:0048469; P:cell maturation; ISO:RGD.
DR GO; GO:0008283; P:cell population proliferation; ISO:RGD.
DR GO; GO:0071773; P:cellular response to BMP stimulus; ISO:RGD.
DR GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IEP:RGD.
DR GO; GO:0002063; P:chondrocyte development; ISO:RGD.
DR GO; GO:0002062; P:chondrocyte differentiation; ISO:RGD.
DR GO; GO:0048701; P:embryonic cranial skeleton morphogenesis; ISO:RGD.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; ISO:RGD.
DR GO; GO:0001958; P:endochondral ossification; ISO:RGD.
DR GO; GO:0010467; P:gene expression; ISO:RGD.
DR GO; GO:0030097; P:hemopoiesis; IBA:GO_Central.
DR GO; GO:0036076; P:ligamentous ossification; ISO:RGD.
DR GO; GO:0045879; P:negative regulation of smoothened signaling pathway; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; ISO:RGD.
DR GO; GO:0001503; P:ossification; IBA:GO_Central.
DR GO; GO:0002076; P:osteoblast development; ISO:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; IMP:RGD.
DR GO; GO:0002051; P:osteoblast fate commitment; ISO:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0032332; P:positive regulation of chondrocyte differentiation; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0045778; P:positive regulation of ossification; IMP:RGD.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:1901522; P:positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0045595; P:regulation of cell differentiation; IBA:GO_Central.
DR GO; GO:0040036; P:regulation of fibroblast growth factor receptor signaling pathway; ISO:RGD.
DR GO; GO:0010468; P:regulation of gene expression; ISO:RGD.
DR GO; GO:0042487; P:regulation of odontogenesis of dentin-containing tooth; ISO:RGD.
DR GO; GO:0030278; P:regulation of ossification; ISO:RGD.
DR GO; GO:0045667; P:regulation of osteoblast differentiation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0033591; P:response to L-ascorbic acid; ISO:RGD.
DR GO; GO:1904383; P:response to sodium phosphate; ISO:RGD.
DR GO; GO:0001501; P:skeletal system development; ISO:RGD.
DR GO; GO:0048705; P:skeletal system morphogenesis; ISO:RGD.
DR GO; GO:0048863; P:stem cell differentiation; ISO:RGD.
DR GO; GO:0030217; P:T cell differentiation; ISO:RGD.
DR Gene3D; 2.60.40.720; -; 1.
DR InterPro; IPR000040; AML1_Runt.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR012346; p53/RUNT-type_TF_DNA-bd_sf.
DR InterPro; IPR013524; Runt_dom.
DR PANTHER; PTHR11950; PTHR11950; 1.
DR Pfam; PF00853; Runt; 1.
DR PRINTS; PR00967; ONCOGENEAML1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS51062; RUNT; 1.
PE 1: Evidence at protein level;
KW Differentiation; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..>218
FT /note="Runt-related transcription factor 2"
FT /id="PRO_0000174661"
FT DOMAIN 67..195
FT /note="Runt"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00399"
FT REGION 122..138
FT /note="Required for interaction with FOXO1"
FT /evidence="ECO:0000250"
FT REGION 189..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..208
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 204
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q13950"
FT NON_CONS 98..99
FT /evidence="ECO:0000305"
FT NON_TER 218
SQ SEQUENCE 218 AA; 24247 MW; D6465458B60628A2 CRC64;
MLHSPHKQPQ NHKCGANFLQ EDSKEALVFK WLISAGHYQP PRPTESVSAL STVHAVIFKA
ASSIYNRGHK FYLEKKGGTM ASNSLFSAVT PCQQSFFWNK TLPVAFKVVA LGEVPDGTVV
TVMAGNDENY SAELRNASAV MKNQVARFND LRFVGRSGRG KSFTLTITVF TNPPQVATYH
RAIKVTVDGP REPRRHRQKL DDSKPSLFSD RLSDLGRI
