ID   RUS1_ACIFR              Reviewed;         155 AA.
AC   P23742; P31372;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2001, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Rusticyanin;
GN   Name=rus;
OS   Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans).
OC   Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales;
OC   Acidithiobacillaceae; Acidithiobacillus.
OX   NCBI_TaxID=920;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1879547; DOI=10.1016/0014-5793(91)81025-4;
RA   Yano T., Fukumori Y., Yamanaka T.;
RT   "The amino acid sequence of rusticyanin isolated from Thiobacillus
RT   ferrooxidans.";
RL   FEBS Lett. 288:159-162(1991).
RN   [2]
RP   PROTEIN SEQUENCE.
RX   PubMed=8448191; DOI=10.1016/0167-4838(93)90123-9;
RA   Nunzi F., Woudstra M., Campese D., Bonicel J., Morin D., Bruschi M.;
RT   "Amino-acid sequence of rusticyanin from Thiobacillus ferrooxidans and its
RT   comparison with other blue copper proteins.";
RL   Biochim. Biophys. Acta 1162:28-34(1993).
CC   -!- FUNCTION: Electron carrier from cytochrome c552 to the A-type oxidase.
CC   -!- COFACTOR:
CC       Name=Cu cation; Xref=ChEBI:CHEBI:23378;
CC       Note=Binds 1 copper ion per subunit.;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Redox potential:
CC         E(0) is +680 mV.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- CAUTION: The two different versions of rusticyanins are most probably
CC       due to strain variations. {ECO:0000305}.
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DR   PIR; S17149; S17149.
DR   PIR; S29825; S29825.
DR   AlphaFoldDB; P23742; -.
DR   SMR; P23742; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   CDD; cd04231; Rusticyanin; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR028871; BlueCu_1_BS.
DR   InterPro; IPR033138; Cu_oxidase_CS.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR001243; Rusticyanin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   PRINTS; PR00158; RUSTICYANIN.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR03095; rusti_cyanin; 1.
DR   PROSITE; PS00196; COPPER_BLUE; 1.
DR   PROSITE; PS00079; MULTICOPPER_OXIDASE1; 1.
PE   1: Evidence at protein level;
KW   Copper; Direct protein sequencing; Electron transport; Metal-binding;
KW   Periplasm; Transport.
FT   CHAIN           1..155
FT                   /note="Rusticyanin"
FT                   /id="PRO_0000085555"
FT   DOMAIN          53..155
FT                   /note="Plastocyanin-like"
FT   BINDING         85
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         138
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         143
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         148
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   CONFLICT        5
FT                   /note="G -> S (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        14
FT                   /note="Q -> E (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        37
FT                   /note="Missing (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   155 AA;  16446 MW;  4EBCD651496C6DDE CRC64;
     GALDGSWKEA TLPQVKAMLQ KDTGKASGDT VTYSGKTVHV VAAAVLPGFP FPSFEVHDKK
     NPTLDIPAGA TVDVTFINTN KGFGHSFDIT KKGPPFAVMP NIKPIVAGTG FSPVPKDGKF
     GYSEFTWHPT AGTYYYVCQI PGHAATGMFG KIIVK