RUS1_ARATH
ID RUS1_ARATH Reviewed; 608 AA.
AC Q7X6P3; Q9LZU1;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein root UVB sensitive 1, chloroplastic {ECO:0000303|PubMed:19075229};
DE AltName: Full=Protein WEAK AUXIN RESPONSE 3 {ECO:0000303|PubMed:23580592};
DE Flags: Precursor;
GN Name=RUS1 {ECO:0000303|PubMed:19075229};
GN Synonyms=WXR3 {ECO:0000303|PubMed:23580592};
GN OrderedLocusNames=At3g45890 {ECO:0000312|Araport:AT3G45890};
GN ORFNames=F16L2.10 {ECO:0000312|EMBL:CAB82813.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAP40410.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=19075229; DOI=10.1073/pnas.0809942106;
RA Tong H., Leasure C.D., Hou X., Yuen G., Briggs W., He Z.H.;
RT "Role of root UV-B sensing in Arabidopsis early seedling development.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:21039-21044(2008).
RN [6]
RP FUNCTION, INTERACTION WITH RUS2, MUTAGENESIS OF LYS-281; GLU-298; ASN-342
RP AND LYS-349, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=19515790; DOI=10.1104/pp.109.139253;
RA Leasure C.D., Tong H., Yuen G., Hou X., Sun X., He Z.H.;
RT "ROOT UV-B SENSITIVE2 acts with ROOT UV-B SENSITIVE1 in a root ultraviolet
RT B-sensing pathway.";
RL Plant Physiol. 150:1902-1915(2009).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=21511809; DOI=10.1093/mp/ssr033;
RA Leasure C.D., Tong H.Y., Hou X.W., Shelton A., Minton M., Esquerra R.,
RA Roje S., Hellmann H., He Z.H.;
RT "root uv-b sensitive mutants are suppressed by specific mutations in
RT ASPARTATE AMINOTRANSFERASE2 and by exogenous vitamin B6.";
RL Mol. Plant 4:759-770(2011).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=23580592; DOI=10.1104/pp.113.217018;
RA Yu H., Karampelias M., Robert S., Peer W.A., Swarup R., Ye S., Ge L.,
RA Cohen J., Murphy A., Friml J., Estelle M.;
RT "ROOT ULTRAVIOLET B-SENSITIVE1/weak auxin response3 is essential for polar
RT auxin transport in Arabidopsis.";
RL Plant Physiol. 162:965-976(2013).
CC -!- FUNCTION: Involved in a root UV-B sensing pathway and in the protection
CC against the hypersensitivity to very low-fluence-rate (VLF) UV-B. RSU1
CC and RUS2 are probably both negative modulators of the same UV-B
CC perception pathway, which when overstimulated in the roots causes a
CC block to postgermination development. Required for polar auxin
CC transport by maintaining the proper levels of auxin transporters AUX1
CC (AC Q96247) and PIN proteins on the plasma membrane.
CC {ECO:0000269|PubMed:19075229, ECO:0000269|PubMed:19515790,
CC ECO:0000269|PubMed:23580592}.
CC -!- SUBUNIT: Interacts (via the DUF647 domain) with RUS2 (via the DUF647
CC domain). {ECO:0000269|PubMed:19515790}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:23580592}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons, roots, hypocotyls, leaf
CC veins, root vascular tissues and root tips. Expressed near the root
CC apical meristem, in the cortex region of the root elongation zone, in
CC lateral roots and emerging lateral roots. Not detected in extreme root
CC apical meristem or root cap. {ECO:0000269|PubMed:19075229,
CC ECO:0000269|PubMed:23580592}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. Extremely stunted growth, failure to develop true
CC postembryonic leaves and arrested primary root elongation, when grown
CC in vitro. {ECO:0000269|PubMed:19075229}.
CC -!- MISCELLANEOUS: Several mutations in ASP2 (AC P46645), but not all, and
CC any of the non-phosphorylated B6 vitamers can suppress the rus
CC phenotype. {ECO:0000269|PubMed:21511809}.
CC -!- SIMILARITY: Belongs to the RUS1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82813.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162459; CAB82813.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78087.1; -; Genomic_DNA.
DR EMBL; BT008583; AAP40410.1; -; mRNA.
DR EMBL; BT008683; AAP40490.1; -; mRNA.
DR EMBL; AK229694; BAF01534.1; -; mRNA.
DR PIR; T47529; T47529.
DR RefSeq; NP_190175.2; NM_114458.4.
DR AlphaFoldDB; Q7X6P3; -.
DR IntAct; Q7X6P3; 1.
DR STRING; 3702.AT3G45890.1; -.
DR PaxDb; Q7X6P3; -.
DR PRIDE; Q7X6P3; -.
DR ProteomicsDB; 226635; -.
DR EnsemblPlants; AT3G45890.1; AT3G45890.1; AT3G45890.
DR GeneID; 823732; -.
DR Gramene; AT3G45890.1; AT3G45890.1; AT3G45890.
DR KEGG; ath:AT3G45890; -.
DR Araport; AT3G45890; -.
DR TAIR; locus:2077197; AT3G45890.
DR eggNOG; KOG4249; Eukaryota.
DR HOGENOM; CLU_015325_7_0_1; -.
DR InParanoid; Q7X6P3; -.
DR OMA; DPGDGWW; -.
DR OrthoDB; 1357597at2759; -.
DR PhylomeDB; Q7X6P3; -.
DR PRO; PR:Q7X6P3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q7X6P3; baseline and differential.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0032502; P:developmental process; IMP:TAIR.
DR GO; GO:0010224; P:response to UV-B; IMP:TAIR.
DR InterPro; IPR006968; RUS_fam.
DR PANTHER; PTHR12770; PTHR12770; 1.
DR Pfam; PF04884; UVB_sens_prot; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..68
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 69..608
FT /note="Protein root UVB sensitive 1, chloroplastic"
FT /id="PRO_0000430818"
FT TRANSMEM 112..132
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT MUTAGEN 281
FT /note="K->G: Loss of interaction with RUS2."
FT /evidence="ECO:0000269|PubMed:19515790"
FT MUTAGEN 298
FT /note="E->G: Decreased interaction with RUS2."
FT /evidence="ECO:0000269|PubMed:19515790"
FT MUTAGEN 342
FT /note="N->G: Decreased interaction with RUS2."
FT /evidence="ECO:0000269|PubMed:19515790"
FT MUTAGEN 349
FT /note="K->G: Loss of interaction with RUS2."
FT /evidence="ECO:0000269|PubMed:19515790"
SQ SEQUENCE 608 AA; 66385 MW; BFF87E5DDE9766B1 CRC64;
MSCSYLLSGD ASVVASRRWS DVSSRQRLFL PRQSLRQPSG SSFSRCVRLV ANVNDHFSKQ
SLATRNCLAS VFSADLGGSN GNNDNGNGGG GGGDGGGDNS DDSSFDLRYL CFLLLGLSCF
FHFRLSAASA IAKDQNSDSN GDAVKETVWE VRGSKRKRLV PDFVKDEFVS EESAFELSSS
LTPENLLAQC RNLLTQFLLP EGFPNSVTSD YLDYSLWRGV QGIASQISGV LATQSLLYAV
GLGKGAIPTA AAINWVLKDG IGYLSKIMLS KYGRHFDVHP KGWRLFADLL ENAAFGMEML
TPVFPQFFVM IGAAAGAGRS AAALIQAATR SCFNAGFASQ RNFAEVIAKG EAQGMVSKSV
GILLGIVVAN CIGTSTSLAL AAFGVVTTIH MYTNLKSYQC IQLRTLNPYR ASLVFSEYLI
SGQAPLIKEV NDEEPLFPTV RFSNMKSPEK LQDFVLSSEA KAAAADIEER LQLGSKLSDV
IHNKEEAIAL FDLYRNEGYI LTEHKGRFCV MLKESSTPQD MLRSLFQVNY LYWLEKNAGI
EPASTYSDCK PGGRLHISLD YVRREFEHAK EDSESVGWVT EGLIARPLPT RIRLGHDREL
LLSSPNSS
