RUS2_ARATH
ID RUS2_ARATH Reviewed; 433 AA.
AC Q9SJX7; F4IQQ5;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Protein root UVB sensitive 2, chloroplastic {ECO:0000303|PubMed:19515790};
DE AltName: Full=Protein WEAK AUXIN RESPONSE 1 {ECO:0000303|PubMed:20562234};
GN Name=RUS2 {ECO:0000303|PubMed:19515790};
GN Synonyms=WXR1 {ECO:0000303|PubMed:20562234};
GN OrderedLocusNames=At2g31190 {ECO:0000312|Araport:AT2G31190,
GN ECO:0000312|EMBL:AEC08505.1};
GN ORFNames=F16D14.3 {ECO:0000312|EMBL:AAD20664.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, INTERACTION WITH RUS1, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=19515790; DOI=10.1104/pp.109.139253;
RA Leasure C.D., Tong H., Yuen G., Hou X., Sun X., He Z.H.;
RT "ROOT UV-B SENSITIVE2 acts with ROOT UV-B SENSITIVE1 in a root ultraviolet
RT B-sensing pathway.";
RL Plant Physiol. 150:1902-1915(2009).
RN [6]
RP FUNCTION, MUTAGENESIS OF SER-105, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=20562234; DOI=10.1105/tpc.110.074195;
RA Ge L., Peer W., Robert S., Swarup R., Ye S., Prigge M., Cohen J.D.,
RA Friml J., Murphy A., Tang D., Estelle M.;
RT "Arabidopsis ROOT UVB SENSITIVE2/WEAK AUXIN RESPONSE1 is required for polar
RT auxin transport.";
RL Plant Cell 22:1749-1761(2010).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=21511809; DOI=10.1093/mp/ssr033;
RA Leasure C.D., Tong H.Y., Hou X.W., Shelton A., Minton M., Esquerra R.,
RA Roje S., Hellmann H., He Z.H.;
RT "root uv-b sensitive mutants are suppressed by specific mutations in
RT ASPARTATE AMINOTRANSFERASE2 and by exogenous vitamin B6.";
RL Mol. Plant 4:759-770(2011).
CC -!- FUNCTION: Involved in a root UV-B sensing pathway and in the protection
CC against the hypersensitivity to very low-fluence-rate (VLF) UV-B. RSU1
CC and RUS2 are probably both negative modulators of the same UV-B
CC perception pathway, which when overstimulated in the roots causes a
CC block to postgermination development. Required for polar auxin
CC transport and to maintain the normal levels of PIN proteins in the
CC root. {ECO:0000269|PubMed:19515790, ECO:0000269|PubMed:20562234}.
CC -!- SUBUNIT: Interacts (via the DUF647 domain) with RUS1 (via the DUF647
CC domain). {ECO:0000269|PubMed:19515790}.
CC -!- SUBCELLULAR LOCATION: Plastid {ECO:0000269|PubMed:20562234,
CC ECO:0000303|PubMed:19515790}. Note=May be localized to the plastid as a
CC consequence of its interaction with RUS1.
CC {ECO:0000303|PubMed:19515790}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SJX7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SJX7-2; Sequence=VSP_057100;
CC -!- TISSUE SPECIFICITY: Expressed throughout the plant, with a higher
CC expression near the root apical meristem, in the cortex region of the
CC root elongation zone, in lateral roots and emerging lateral roots. Not
CC detected in extreme root apical meristem or root cap.
CC {ECO:0000269|PubMed:19515790, ECO:0000269|PubMed:20562234}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. Extremely stunted growth, failure to develop true
CC postembryonic leaves and arrested primary root elongation, when grown
CC in vitro. {ECO:0000269|PubMed:19515790}.
CC -!- MISCELLANEOUS: Several mutations in ASP2 (AC P46645), but not all, and
CC any of the non-phosphorylated B6 vitamers can suppress the rus
CC phenotype. {ECO:0000269|PubMed:21511809}.
CC -!- SIMILARITY: Belongs to the RUS1 family. {ECO:0000305}.
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DR EMBL; AC006593; AAD20664.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08505.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08506.1; -; Genomic_DNA.
DR EMBL; AY085428; AAM62655.1; -; mRNA.
DR EMBL; BT025987; ABG25076.1; -; mRNA.
DR PIR; F84717; F84717.
DR RefSeq; NP_001189649.1; NM_001202720.1. [Q9SJX7-2]
DR RefSeq; NP_565718.1; NM_128675.3. [Q9SJX7-1]
DR AlphaFoldDB; Q9SJX7; -.
DR BioGRID; 3023; 1.
DR IntAct; Q9SJX7; 2.
DR STRING; 3702.AT2G31190.1; -.
DR PaxDb; Q9SJX7; -.
DR PRIDE; Q9SJX7; -.
DR ProteomicsDB; 226636; -. [Q9SJX7-1]
DR EnsemblPlants; AT2G31190.1; AT2G31190.1; AT2G31190. [Q9SJX7-1]
DR EnsemblPlants; AT2G31190.2; AT2G31190.2; AT2G31190. [Q9SJX7-2]
DR GeneID; 817675; -.
DR Gramene; AT2G31190.1; AT2G31190.1; AT2G31190. [Q9SJX7-1]
DR Gramene; AT2G31190.2; AT2G31190.2; AT2G31190. [Q9SJX7-2]
DR KEGG; ath:AT2G31190; -.
DR Araport; AT2G31190; -.
DR TAIR; locus:2042536; AT2G31190.
DR eggNOG; KOG4249; Eukaryota.
DR InParanoid; Q9SJX7; -.
DR OMA; KYPHFST; -.
DR PhylomeDB; Q9SJX7; -.
DR PRO; PR:Q9SJX7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJX7; baseline and differential.
DR Genevisible; Q9SJX7; AT.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009536; C:plastid; IDA:TAIR.
DR GO; GO:0009926; P:auxin polar transport; IMP:TAIR.
DR GO; GO:0010224; P:response to UV-B; IMP:TAIR.
DR InterPro; IPR006968; RUS_fam.
DR PANTHER; PTHR12770; PTHR12770; 1.
DR Pfam; PF04884; UVB_sens_prot; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Plastid; Reference proteome.
FT CHAIN 1..433
FT /note="Protein root UVB sensitive 2, chloroplastic"
FT /id="PRO_0000430819"
FT VAR_SEQ 5
FT /note="Missing (in isoform 2)"
FT /id="VSP_057100"
FT MUTAGEN 105
FT /note="S->L: In wxr1; reduced auxin transport from the
FT shoot to the root."
FT /evidence="ECO:0000269|PubMed:20562234"
SQ SEQUENCE 433 AA; 48259 MW; 79538564A9B5D55B CRC64;
MQFLQEKVKL IKKEDPVMLK SPEDFPVYWF ETSDSVSHRY QFQSDGHLSM KVVDDARPVP
QKMVESFLNK FFPSGYPYSV NEGYLRYTQF RALQHFSSAA LSVLSTQSLL FAAGLRPTPA
QATVVSWILK DGMQHVGKLI CSNLGARMDS EPKRWRILAD VLYDLGTGLE LVSPLCPHLF
LEMAGLGNFA KGMATVAARA TRLPIYSSFA KEGNLSDIFA KGEAISTLFN VAGIGAGIQL
ASTICSSMEG KLVVGSILSV VHVYSVVEQM RGVPINTLNP QRTALIVANF LKTGKVPSPP
DLRFQEDLMF PERPIQDAGN VKVGRALHKA VKPSEVQRLK QVFVEEKFLL SHGKSWTDMV
LEHDATGEDA LRGWLVAAYV KSMTKIYNDP DDIILQDAYD KMNDVFNPFL SQVQAKGWYT
DRFLDGTGTR FAW
