ID   RUSA_BP82               Reviewed;         120 AA.
AC   Q37873;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Crossover junction endodeoxyribonuclease rusA;
DE            EC=3.1.21.10;
DE   AltName: Full=Holliday junction nuclease rusA;
DE   AltName: Full=Holliday junction resolvase;
GN   Name=rusA; Synonyms=rus;
OS   Enterobacteria phage 82 (Bacteriophage 82).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus.
OX   NCBI_TaxID=10705;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8648624; DOI=10.1006/jmbi.1996.0185;
RA   Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.;
RT   "Holliday junction resolvases encoded by homologous rusA genes in
RT   Escherichia coli K-12 and phage 82.";
RL   J. Mol. Biol. 257:561-573(1996).
CC   -!- FUNCTION: Endonuclease that resolves Holliday junction intermediates.
CC       Promotes DNA repair. Exhibits sequence and structure-selective cleavage
CC       of four-way DNA junctions, where it introduces symmetrical nicks in two
CC       strands of the same polarity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC         single-stranded crossover between two homologous DNA duplexes
CC         (Holliday junction).; EC=3.1.21.10;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RusA family. {ECO:0000305}.
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DR   EMBL; X92588; CAA63330.1; -; Genomic_DNA.
DR   PIR; S66583; S66583.
DR   SMR; Q37873; -.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1330.70; -; 1.
DR   InterPro; IPR016281; Endonuclease_RusA.
DR   InterPro; IPR008822; Endonuclease_RusA-like.
DR   InterPro; IPR036614; RusA-like_sf.
DR   Pfam; PF05866; RusA; 1.
DR   PIRSF; PIRSF001007; RusA; 1.
DR   SUPFAM; SSF103084; SSF103084; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW   Magnesium; Metal-binding; Nuclease.
FT   CHAIN           1..120
FT                   /note="Crossover junction endodeoxyribonuclease rusA"
FT                   /id="PRO_0000192006"
FT   BINDING         70
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         72
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         91
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   120 AA;  13885 MW;  5E70726964490A7F CRC64;
     MNTYHITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG LAMPVKIRIE
     CHMPDRRRRD LDNLQKASFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE