ID   RUSA_BPHK7              Reviewed;         120 AA.
AC   Q9MCN8;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Crossover junction endodeoxyribonuclease rusA;
DE            EC=3.1.21.10;
DE   AltName: Full=Gp67;
DE   AltName: Full=Holliday junction nuclease rusA;
DE   AltName: Full=Holliday junction resolvase;
GN   Name=rusA; Synonyms=67;
OS   Enterobacteria phage HK97 (Bacteriophage HK97).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Hendrixvirinae; Byrnievirus.
OX   NCBI_TaxID=2681617;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10860721; DOI=10.1006/jmbi.2000.3729;
RA   Juhala R.J., Ford M.E., Duda R.L., Youlton A., Hatfull G.F., Hendrix R.W.;
RT   "Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic
RT   mosaicism in the lambdoid bacteriophages.";
RL   J. Mol. Biol. 299:27-51(2000).
CC   -!- FUNCTION: Endonuclease that resolves Holliday junction intermediates.
CC       Promotes DNA repair. Exhibits sequence and structure-selective cleavage
CC       of four-way DNA junctions, where it introduces symmetrical nicks in two
CC       strands of the same polarity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC         single-stranded crossover between two homologous DNA duplexes
CC         (Holliday junction).; EC=3.1.21.10;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RusA family. {ECO:0000305}.
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DR   EMBL; AF069529; AAF31142.1; -; Genomic_DNA.
DR   RefSeq; NP_037749.1; NC_002167.1.
DR   SMR; Q9MCN8; -.
DR   GeneID; 1262586; -.
DR   KEGG; vg:1262586; -.
DR   Proteomes; UP000002576; Genome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1330.70; -; 1.
DR   InterPro; IPR016281; Endonuclease_RusA.
DR   InterPro; IPR008822; Endonuclease_RusA-like.
DR   InterPro; IPR036614; RusA-like_sf.
DR   Pfam; PF05866; RusA; 1.
DR   PIRSF; PIRSF001007; RusA; 1.
DR   SUPFAM; SSF103084; SSF103084; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW   Magnesium; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..120
FT                   /note="Crossover junction endodeoxyribonuclease rusA"
FT                   /id="PRO_0000192007"
FT   BINDING         70
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         72
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         91
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   120 AA;  14067 MW;  7D28A53BA6ED7398 CRC64;
     MNEYQFVLPY PPSLNTYWRR RGSQYYISDK GQKYRKDVQQ IIRQLKLDIF TKSRLRIKVI
     ADVPDSRRRD LDNILKGLLD SLIHAGFAED DEQFDDIRVI RGVKVPGGRL GIKITELESV