ID   RUSA_ECO57              Reviewed;         120 AA.
AC   Q8X707; Q7AF07;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Crossover junction endodeoxyribonuclease RusA;
DE            EC=3.1.21.10;
DE   AltName: Full=Holliday junction nuclease RusA;
DE   AltName: Full=Holliday junction resolvase;
GN   Name=rusA; OrderedLocusNames=Z1873, ECs1619;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Endonuclease that resolves Holliday junction intermediates
CC       made during homologous genetic recombination and DNA repair. Exhibits
CC       sequence and structure-selective cleavage of four-way DNA junctions,
CC       where it introduces symmetrical nicks in two strands of the same
CC       polarity at the 5' side of CC dinucleotides. Corrects the defects in
CC       genetic recombination and DNA repair associated with inactivation of
CC       RuvAB or RuvC (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC         single-stranded crossover between two homologous DNA duplexes
CC         (Holliday junction).; EC=3.1.21.10;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Is part of the cryptic phage CP-933X.
CC   -!- SIMILARITY: Belongs to the RusA family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG55970.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35042.1; -; Genomic_DNA.
DR   PIR; C90831; C90831.
DR   PIR; F85688; F85688.
DR   RefSeq; NP_309646.1; NC_002695.1.
DR   RefSeq; WP_001099695.1; NZ_SDVX01000011.1.
DR   AlphaFoldDB; Q8X707; -.
DR   SMR; Q8X707; -.
DR   STRING; 155864.EDL933_1815; -.
DR   EnsemblBacteria; AAG55970; AAG55970; Z1873.
DR   EnsemblBacteria; BAB35042; BAB35042; ECs_1619.
DR   GeneID; 913268; -.
DR   KEGG; ece:Z1873; -.
DR   KEGG; ecs:ECs_1619; -.
DR   PATRIC; fig|386585.9.peg.1717; -.
DR   eggNOG; COG4570; Bacteria.
DR   HOGENOM; CLU_139466_0_2_6; -.
DR   OMA; KEQMLDI; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1330.70; -; 1.
DR   InterPro; IPR016281; Endonuclease_RusA.
DR   InterPro; IPR008822; Endonuclease_RusA-like.
DR   InterPro; IPR036614; RusA-like_sf.
DR   Pfam; PF05866; RusA; 1.
DR   PIRSF; PIRSF001007; RusA; 1.
DR   SUPFAM; SSF103084; SSF103084; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW   Magnesium; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..120
FT                   /note="Crossover junction endodeoxyribonuclease RusA"
FT                   /id="PRO_0000324835"
FT   REGION          13..16
FT                   /note="DNA-binding"
FT                   /evidence="ECO:0000250"
FT   REGION          66..73
FT                   /note="DNA-binding"
FT                   /evidence="ECO:0000250"
FT   BINDING         70
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         72
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         91
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   120 AA;  13773 MW;  5E6B65CA32230A7F CRC64;
     MNTYSITLPW PPSNNRYYRH NRGRTHISAE GQAYRDNVAR IIKGSMLDIG LAMPVKIRIE
     CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGKL ELTITELGNE