RUSC2_MOUSE
ID RUSC2_MOUSE Reviewed; 1514 AA.
AC Q80U22; Q8R1D6;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=AP-4 complex accessory subunit RUSC2 {ECO:0000305};
DE AltName: Full=Interacting protein of Rab1 {ECO:0000250|UniProtKB:Q8N2Y8};
DE Short=Iporin {ECO:0000250|UniProtKB:Q8N2Y8};
DE AltName: Full=RUN and SH3 domain-containing protein 2;
GN Name=Rusc2 {ECO:0000312|MGI:MGI:2140371};
GN Synonyms=Kiaa0375 {ECO:0000303|PubMed:12693553};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 857-1514 (ISOFORM 2).
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Associates with the adapter-like complex 4 (AP-4) and may
CC therefore play a role in vesicular trafficking of proteins at the
CC trans-Golgi network. {ECO:0000250|UniProtKB:Q8N2Y8}.
CC -!- SUBUNIT: Associated component of the adapter-like complex 4 (AP-4).
CC Interacts with active RAB1A and RAB1B, and with GOLGA2.
CC {ECO:0000250|UniProtKB:Q8N2Y8}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q8N2Y8}. Note=Cytosolic punctate distribution.
CC Also observed in the perinuclear region (By similarity).
CC {ECO:0000250|UniProtKB:Q8N2Y8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80U22-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80U22-2; Sequence=VSP_010858, VSP_010859;
CC -!- DOMAIN: The RUN domain is required for the interaction with RAB1A and
CC RAB1B. {ECO:0000250|UniProtKB:Q8N2Y8}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65545.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK122263; BAC65545.1; ALT_INIT; mRNA.
DR EMBL; BC024790; AAH24790.1; -; mRNA.
DR AlphaFoldDB; Q80U22; -.
DR SMR; Q80U22; -.
DR STRING; 10090.ENSMUSP00000095710; -.
DR iPTMnet; Q80U22; -.
DR PhosphoSitePlus; Q80U22; -.
DR jPOST; Q80U22; -.
DR MaxQB; Q80U22; -.
DR PaxDb; Q80U22; -.
DR PRIDE; Q80U22; -.
DR ProteomicsDB; 256846; -. [Q80U22-1]
DR ProteomicsDB; 256847; -. [Q80U22-2]
DR MGI; MGI:2140371; Rusc2.
DR eggNOG; ENOG502QR1I; Eukaryota.
DR InParanoid; Q80U22; -.
DR ChiTaRS; Rusc2; mouse.
DR PRO; PR:Q80U22; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q80U22; protein.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR Gene3D; 1.20.58.900; -; 1.
DR InterPro; IPR004012; Run_dom.
DR InterPro; IPR037213; Run_dom_sf.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF02759; RUN; 1.
DR Pfam; PF14604; SH3_9; 1.
DR SMART; SM00593; RUN; 1.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF140741; SSF140741; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR PROSITE; PS50826; RUN; 1.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome;
KW SH3 domain.
FT CHAIN 1..1514
FT /note="AP-4 complex accessory subunit RUSC2"
FT /id="PRO_0000097535"
FT DOMAIN 1026..1170
FT /note="RUN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT DOMAIN 1445..1504
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT REGION 67..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 228..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 402..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 476..510
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 547..603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 642..683
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..832
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1204..1255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1279..1405
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1423..1446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 402..423
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 476..494
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 561..576
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 769..832
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1279..1298
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1352..1366
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 534
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N2Y8"
FT MOD_RES 541
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N2Y8"
FT MOD_RES 557
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N2Y8"
FT MOD_RES 651
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N2Y8"
FT MOD_RES 776
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N2Y8"
FT MOD_RES 1378
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N2Y8"
FT VAR_SEQ 1448..1453
FT /note="CEVQAL -> WYAQGN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010858"
FT VAR_SEQ 1454..1514
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010859"
SQ SEQUENCE 1514 AA; 161139 MW; 90E8E0FE3A42BFE1 CRC64;
MDSPPKLTGE TLIVHHIPLV HCQVPDRQCC GGAGGGGGGT RANPFCPPEL GLTQPDHDLG
QADSLLYPSL HSAPGAPTGS SDSVKSRSRD GRGPGAPKRH NPFLVQEGVG ETGLGDLHDS
STGDSVTQQS FHLHSASQPF HLSSFQLPPS GPGQGRPWGA THSRPGVVEG QEQDPATALG
TQCSTSHCCR PELEAERMEL DECGGHGGSG SGGGTSDISG FSFEQEWKIS SDESPRHPGR
SGSGTQHCHC SSTSSQSEAA DQSMGYVSDS SCNSSDGVLV TFSTLYNKMH SSSRANLNSV
PQSCSDSSFC SHADPGAFYL DLQPSPAESR MSCESHHPEN GDREEGCGCP HVSSPELDAN
CNAYHPHSEP CPAVADLTAC FQSQARLVVA TQNYYKLVTC DLSSQSSPSP AGSSITSCSE
EHTKISPPPG PCPDPDPNQP SEYYLFQKPD IQPEEQEAVG SPAEAATAMG PTVLEGQVYT
NTSPPNLNTG RQRSRSYDRS LERSPPVRLG SLERMLSCPV RLSEGPAALA GPASPPRRVT
SFAELAKGRK KAAGSGSPPL RASVGDSSQE FSPIQEAQQD RAAPLDEGTR CSHSLPSLPL
GPSLDLLGPE PWSTPVCQGS QSSEMPLPSL RAAGQGPLAQ LMDPGPAFSG SPATSHTQRD
SRARADGGGT ESRPVLRYSK EQRPTTLPIQ PFVFQHHFPK QLAKARALHS LSQLYSLSGC
SRAQQPAPLV ISTAQGPAPA PSGEPQPFTS QASGRGARNA GPEPETSRPS PLGSYSPVRS
AGPFGSSTDS SASTSCSPPP EQGTAADSVS PWSHTCPPTV RPATSQQPPK EDQKIPTLAE
YRLHGTGSLP PLGSWRSGFT RAESLVRGGG EGSMANRPNN ANHLSPQALK WREYRRKNPL
GPPGLSGSLD RRPPEARLAR RNPIFEFPGS FGTTSHLNCR LNGQISKPLS LTCPDLQDPF
SLTEKPPAEF CLSPDGNSEA ISIDILQKKG LVKAVNTAVD LIVAHFGTSR DPGVKAKLGN
SSVSPNVGHL VLKYLCPAVQ AVLEDGLKAF VLDVIIGQRK NMPWSVVEAS TQLGPSTKVL
HGLYNKVSQF PELTSHTMRF NAFILGLLNI RSLEFWFNHL YNHEDIIQTH YQPWGFLRAA
HTVCPGLFEE LLLLLQPLAL LPFSLDLLFQ HRLLQSGRQQ RQHKELLRVS QDLLLSAHST
LQLARSRGQE GPGDMDRVAP GERVKGVGAS EGGEEDEEDA EEVAVVAGSS DHGKWARGGQ
AGWWYQLMQS SQVYIDGTAE GSRFPRSSSS SSGSGSEKKK GVGSGGPSQA PPPPPREGVV
EGAEACPAPE EALGQERGWP FWMGSPPDSV LAELRRSRER EGPVAPPTEN EEGTAEPSPG
GIKWGHLFGS RKSQREARPT NRLPSDWLSL DKSVFQLVAQ TMGARREPEP RENLQESHPP
AVPSKPPCEV QALCHHLATG PGQLSFHKGD ILRVLGPARG DWLRCSRGPD TGLVPLAYVT
LPPTPSSPPG SSQN
