RUSD2_MOUSE
ID RUSD2_MOUSE Reviewed; 553 AA.
AC Q149F1; Q8BXL2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Pseudouridylate synthase RPUSD2 {ECO:0000305};
DE EC=5.4.99.- {ECO:0000250|UniProtKB:Q8IZ73};
DE AltName: Full=RNA pseudouridylate synthase domain-containing protein 2 {ECO:0000305};
GN Name=Rpusd2 {ECO:0000312|MGI:MGI:1918066};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-490, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Pseudouridine synthase that catalyzes pseudouridylation of
CC mRNAs. {ECO:0000250|UniProtKB:Q8IZ73}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in mRNA = a pseudouridine in mRNA;
CC Xref=Rhea:RHEA:56644, Rhea:RHEA-COMP:14658, Rhea:RHEA-COMP:14659,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000250|UniProtKB:Q8IZ73};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; AK044767; BAC32077.1; -; mRNA.
DR EMBL; AL772264; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC117827; AAI17828.1; -; mRNA.
DR EMBL; BC117828; AAI17829.1; -; mRNA.
DR CCDS; CCDS16588.1; -.
DR RefSeq; NP_775626.1; NM_173450.3.
DR AlphaFoldDB; Q149F1; -.
DR SMR; Q149F1; -.
DR BioGRID; 234852; 7.
DR STRING; 10090.ENSMUSP00000028796; -.
DR iPTMnet; Q149F1; -.
DR PhosphoSitePlus; Q149F1; -.
DR EPD; Q149F1; -.
DR jPOST; Q149F1; -.
DR MaxQB; Q149F1; -.
DR PaxDb; Q149F1; -.
DR PeptideAtlas; Q149F1; -.
DR PRIDE; Q149F1; -.
DR ProteomicsDB; 256661; -.
DR Antibodypedia; 10143; 152 antibodies from 21 providers.
DR DNASU; 271842; -.
DR Ensembl; ENSMUST00000028796; ENSMUSP00000028796; ENSMUSG00000027324.
DR GeneID; 271842; -.
DR KEGG; mmu:271842; -.
DR UCSC; uc008lsz.2; mouse.
DR CTD; 27079; -.
DR MGI; MGI:1918066; Rpusd2.
DR VEuPathDB; HostDB:ENSMUSG00000027324; -.
DR eggNOG; KOG1919; Eukaryota.
DR GeneTree; ENSGT00420000029802; -.
DR HOGENOM; CLU_016902_12_5_1; -.
DR InParanoid; Q149F1; -.
DR OMA; VAYHDEM; -.
DR OrthoDB; 821308at2759; -.
DR PhylomeDB; Q149F1; -.
DR TreeFam; TF323255; -.
DR BioGRID-ORCS; 271842; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q149F1; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q149F1; protein.
DR Bgee; ENSMUSG00000027324; Expressed in lumbar dorsal root ganglion and 144 other tissues.
DR Genevisible; Q149F1; MM.
DR GO; GO:0009982; F:pseudouridine synthase activity; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; ISO:MGI.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
PE 1: Evidence at protein level;
KW Isomerase; mRNA processing; Phosphoprotein; Reference proteome.
FT CHAIN 1..553
FT /note="Pseudouridylate synthase RPUSD2"
FT /id="PRO_0000300820"
FT REGION 76..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..135
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 287
FT /evidence="ECO:0000250|UniProtKB:P0AA37"
FT MOD_RES 490
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 237
FT /note="H -> R (in Ref. 3; AAI17828/AAI17829)"
FT /evidence="ECO:0000305"
FT CONFLICT 360
FT /note="V -> M (in Ref. 3; AAI17828/AAI17829)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 553 AA; 61535 MW; 995678B1C9FC4EC8 CRC64;
MWRGVPGCLR DIVQWQVALW SHSFVRTWGS CGKAMTEALS AQAEAAGGLK ALVQPNGDAG
SNTSGEPLLE RLEPAAVGKQ VPESGDQAQG GEGQLPSNGE QTPAPVADSG KRKKRRGATG
ERVVPPPKKR RTGVSFSDEH FAETTYYFEG GLRKVRPYYF DFQTYCKGRW VGRSLLHVFS
TEFRSQPLSY YEAAVRAGRL HLNEEPVQDL SIVLKDNDFL RNTVHRHEPP VTAEPIHLLA
ENNDVVVIDK PSSIPVHPCG RFRHNTVIFI LGKEHQLKEL HPLHRLDRLT SGVLMFAKTA
AVSEKIHEQV RDRQLEKEYV CRVAGEFPDK EVICKEPILV VSYKVGVCRV DPRGKPCETV
FQRLSYNGRS SVVQCRPLTG RTHQIRVHLQ FLGHPILNDP IYNSTAWGPS RGQGGHIPKT
DEELLRDLVA EHQAKESLSM LDLCESDLTP GLIDSTAPSS ELAKDSLEGL ATAAQKIDGI
AEAAPQHLDT PEKAAKADVT QETDPLCAEC RVLRQDPLPQ DLVMFLHALR YKGPDFEYVS
PIPAWARDDW QED
