ID   RUS_ACIF2               Reviewed;         187 AA.
AC   B7JAQ0; P24930; P74919; Q56297; Q56298;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Rusticyanin;
DE   Flags: Precursor;
GN   Name=rus; Synonyms=rusTA; OrderedLocusNames=AFE_3146;
OS   Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768
OS   / NCIMB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)).
OC   Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales;
OC   Acidithiobacillaceae; Acidithiobacillus.
OX   NCBI_TaxID=243159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455;
RX   PubMed=19077236; DOI=10.1186/1471-2164-9-597;
RA   Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II,
RA   Eisen J.A., Holmes D.S.;
RT   "Acidithiobacillus ferrooxidans metabolism: from genome sequence to
RT   industrial applications.";
RL   BMC Genomics 9:597-597(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-187.
RX   PubMed=8935662; DOI=10.1111/j.1574-6968.1996.tb08087.x;
RA   Hall J.F., Hasnain S.S., Ingledew W.J.;
RT   "The structural gene for rusticyanin from Thiobacillus ferrooxidans:
RT   cloning and sequencing of the rusticyanin gene.";
RL   FEMS Microbiol. Lett. 137:85-89(1996).
RN   [3]
RP   PROTEIN SEQUENCE OF 33-187.
RX   PubMed=1892844; DOI=10.1021/bi00103a007;
RA   Ronk M., Shively J.E., Shute E.A., Blake R.C. II;
RT   "Amino acid sequence of the blue copper protein rusticyanin from
RT   Thiobacillus ferrooxidans.";
RL   Biochemistry 30:9435-9442(1991).
CC   -!- FUNCTION: Electron carrier from cytochrome c552 to the A-type oxidase.
CC   -!- COFACTOR:
CC       Name=Cu cation; Xref=ChEBI:CHEBI:23378; Evidence={ECO:0000250};
CC       Note=Binds 1 copper ion per subunit. {ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Redox potential:
CC         E(0) is +680 mV.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
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DR   EMBL; CP001219; ACK80662.1; -; Genomic_DNA.
DR   EMBL; X95324; CAA64630.1; -; Genomic_DNA.
DR   PIR; A40302; CUBCRT.
DR   RefSeq; WP_012537622.1; NC_011761.1.
DR   AlphaFoldDB; B7JAQ0; -.
DR   SMR; B7JAQ0; -.
DR   STRING; 243159.AFE_3146; -.
DR   PaxDb; B7JAQ0; -.
DR   EnsemblBacteria; ACK80662; ACK80662; AFE_3146.
DR   GeneID; 66434079; -.
DR   KEGG; afr:AFE_3146; -.
DR   eggNOG; COG4454; Bacteria.
DR   HOGENOM; CLU_1444789_0_0_6; -.
DR   OMA; GPPYPYM; -.
DR   OrthoDB; 1735306at2; -.
DR   Proteomes; UP000001362; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   CDD; cd04231; Rusticyanin; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR028871; BlueCu_1_BS.
DR   InterPro; IPR033138; Cu_oxidase_CS.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR001243; Rusticyanin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   PRINTS; PR00158; RUSTICYANIN.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR03095; rusti_cyanin; 1.
DR   PROSITE; PS00196; COPPER_BLUE; 1.
DR   PROSITE; PS00079; MULTICOPPER_OXIDASE1; 1.
PE   1: Evidence at protein level;
KW   Copper; Direct protein sequencing; Electron transport; Metal-binding;
KW   Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000269|PubMed:1892844"
FT   CHAIN           33..187
FT                   /note="Rusticyanin"
FT                   /id="PRO_0000369192"
FT   DOMAIN          85..187
FT                   /note="Plastocyanin-like"
FT   BINDING         117
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         170
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         175
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         180
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   187 AA;  19857 MW;  8F7DC1DA5C992CF1 CRC64;
     MYTQNTMKKN WYVTVGAAAA LAATVGMGTA MAGTLDTTWK EATLPQVKAM LEKDTGKVSG
     DTVTYSGKTV HVVAAAVLPG FPFPSFEVHD KKNPTLEIPA GATVDVTFIN TNKGFGHSFD
     ITKKGPPYAV MPVIDPIVAG TGFSPVPKDG KFGYTDFTWH PTAGTYYYVC QIPGHAATGM
     FGKIVVK