RUTA_ECOKI
ID RUTA_ECOKI Reviewed; 393 AA.
AC D5CZH2;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Pyrimidine monooxygenase RutA {ECO:0000255|HAMAP-Rule:MF_01699};
DE EC=1.14.99.46 {ECO:0000255|HAMAP-Rule:MF_01699};
GN Name=rutA {ECO:0000255|HAMAP-Rule:MF_01699}; OrderedLocusNames=ECOK1_1064;
OS Escherichia coli O18:K1:H7 (strain IHE3034 / ExPEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=714962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IHE3034 / ExPEC;
RX PubMed=20439758; DOI=10.1073/pnas.0915077107;
RA Moriel D.G., Bertoldi I., Spagnuolo A., Marchi S., Rosini R., Nesta B.,
RA Pastorello I., Corea V.A., Torricelli G., Cartocci E., Savino S.,
RA Scarselli M., Dobrindt U., Hacker J., Tettelin H., Tallon L.J.,
RA Sullivan S., Wieler L.H., Ewers C., Pickard D., Dougan G., Fontana M.R.,
RA Rappuoli R., Pizza M., Serino L.;
RT "Identification of protective and broadly conserved vaccine antigens from
RT the genome of extraintestinal pathogenic Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:9072-9077(2010).
CC -!- FUNCTION: Catalyzes the pyrimidine ring opening between N-3 and C-4 by
CC an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen
CC atoms in the process to yield ureidoacrylate peracid, that immediately
CC reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN-
CC N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the
CC flavin reductase RutF to regenerate FMN in vivo. {ECO:0000255|HAMAP-
CC Rule:MF_01699}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+)
CC + H2O + NAD(+); Xref=Rhea:RHEA:31587, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17568,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:59891; EC=1.14.99.46;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01699};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN
CC + H(+) + H2O + NAD(+); Xref=Rhea:RHEA:31599, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17821,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:143783; EC=1.14.99.46;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01699};
CC -!- INDUCTION: Up-regulated by the nitrogen regulatory protein C (NtrC also
CC called GlnG) and repressed by RutR. {ECO:0000255|HAMAP-Rule:MF_01699}.
CC -!- SIMILARITY: Belongs to the NtaA/SnaA/DszA monooxygenase family. RutA
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01699}.
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DR EMBL; CP001969; ADE91825.1; -; Genomic_DNA.
DR AlphaFoldDB; D5CZH2; -.
DR SMR; D5CZH2; -.
DR KEGG; eih:ECOK1_1064; -.
DR PATRIC; fig|714962.3.peg.1078; -.
DR HOGENOM; CLU_027853_1_1_6; -.
DR OMA; ADYNFCF; -.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0052614; F:uracil oxygenase activity; IEA:RHEA.
DR GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.30; -; 1.
DR HAMAP; MF_01699; RutA; 1.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR019914; Pyrimidine_monooxygenase_RutA.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; SSF51679; 1.
DR TIGRFAMs; TIGR03612; RutA; 1.
PE 3: Inferred from homology;
KW Flavoprotein; FMN; Monooxygenase; NADP; Oxidoreductase.
FT CHAIN 1..393
FT /note="Pyrimidine monooxygenase RutA"
FT /id="PRO_0000402612"
FT BINDING 79..80
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01699"
FT BINDING 145
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01699"
FT BINDING 154
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01699"
FT BINDING 170..171
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01699"
FT BINDING 220
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01699"
SQ SEQUENCE 393 AA; 43481 MW; A6241D961420BFAC CRC64;
MQTSHYAAEK DMQDAAPRLT FTLRDEERLM MKIGVFVPIG NNGWLISTHA PQYMPTFELN
KAIVQKAEHY HFDFALSMIK LRGFGGKTEF WDHNLESFTL MAGLAAVTSR IQIYATAATL
TLPPAIVARM AATIDSISGG RFGVNLVTGW QKPEYEQMGI WPGDDYFSRR YDYLTEYVQV
LRDLWGSGKS DFKGDFFTMD DCRVSPQPSV PMKVICAGQS DAGMAFSARY ADFNFCFGKG
VNTPTAFAPT AARMKQAAEQ TGRDVGSYVL FMVIADETDD AARAKWEHYK AGADEEALSW
LTEQSQKDTR SGTDTNVRQM ADPTSAVNIN MGTLVGSYAS VARMLDEVAS VPGAEGVLLT
FDDFLSGIEN FGERIQPLMQ CRAHLPALTQ EVA