ID   BCSB_ECO57              Reviewed;         785 AA.
AC   Q8X5L8;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Cyclic di-GMP-binding protein;
DE   AltName: Full=Cellulose synthase regulatory subunit;
DE   Flags: Precursor;
GN   Name=bcsB; OrderedLocusNames=Z4947, ECs4412;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Binds the cellulose synthase activator, bis-(3'-5') cyclic
CC       diguanylic acid (c-di-GMP). {ECO:0000250}.
CC   -!- PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
CC   -!- SUBUNIT: Tightly associated with the cellulose synthase catalytic
CC       subunit. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       type I membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AcsB/BcsB family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG58674.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG58674.2; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB37835.1; -; Genomic_DNA.
DR   PIR; D91180; D91180.
DR   PIR; F86026; F86026.
DR   RefSeq; NP_312439.1; NC_002695.1.
DR   RefSeq; WP_001302734.1; NZ_SDVX01000004.1.
DR   AlphaFoldDB; Q8X5L8; -.
DR   SMR; Q8X5L8; -.
DR   STRING; 155864.EDL933_4787; -.
DR   EnsemblBacteria; AAG58674; AAG58674; Z4947.
DR   EnsemblBacteria; BAB37835; BAB37835; ECs_4412.
DR   GeneID; 915722; -.
DR   KEGG; ece:Z4947; -.
DR   KEGG; ecs:ECs_4412; -.
DR   PATRIC; fig|386585.9.peg.4613; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_003556_1_1_6; -.
DR   UniPathway; UPA00694; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030244; P:cellulose biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006011; P:UDP-glucose metabolic process; IEA:InterPro.
DR   InterPro; IPR003920; Cell_synth_B.
DR   InterPro; IPR018513; Cell_synthase_bac.
DR   PANTHER; PTHR39083; PTHR39083; 1.
DR   Pfam; PF03170; BcsB; 1.
DR   PRINTS; PR01440; CELLSNTHASEB.
PE   3: Inferred from homology;
KW   c-di-GMP; Cell inner membrane; Cell membrane; Cellulose biosynthesis;
KW   Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..785
FT                   /note="Cyclic di-GMP-binding protein"
FT                   /id="PRO_0000000269"
FT   TOPO_DOM        26..744
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        745..765
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        766..785
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   785 AA;  86675 MW;  CE86E566C282E28D CRC64;
     MKRKLFWICA VAMGMSAFPS FMTQATPATQ PLINAEPAVA AQTEQNPQVG QVMPGVQGAD
     APVVAQNGPS RDVKLTFAQI APPPGSMVLR GINPNGSIEF GMRSDEVVTK AMLNLEYTPS
     PSLLPVQSQL KVYLNDELMG VLPVTKEQLG KKTLAQMPIN PLFITDFNRV RLEFVGHYQD
     VCENPASTTL WLDVGRSSGL DLTYQTLNVK NDLSHFPVPF FDPRDNRTNT LPMVFAGAPD
     VGLQQASAIV ASWFGSRSGW RGQNFPVLYN QLPDRNAIVF ATNDKRPDFL RDHPAVKAPV
     IEMINHPQNP YVKLLVVFGR DDKDLLQAAK GIAQGNILFR GESVVVNEVK PLLPRKPYDA
     PNWVRTDRPV TFGELKTYEE QLQSSGLEPA AINVSLNLPP DLYLMRSTGI DMDINYRYTM
     PPVKDSSRMD ISLNNQFLQS FNLSSKQEAN RLLLRIPVLQ GLLDGKTDVS IPALKLGATN
     QLRFDFEYMN PMPGGSVDNC ITFQPVQNHV VIGDDSTIDF SKYYHFIPMP DLRAFANAGF
     PFSRMADLSQ TITVMPKTPN EAQMETLLNT VGFIGAQTGF PAINLTVTDD GSTIQGKDAD
     IMIIGGIPDK LKDDKQIDLL VQATESWVKT PMRQTPFPGI VPDESDRAAE TQSTLTSSGA
     MAAVIGFQSP YNDQRSVIAL LADSPRGYEM LNDAVNDSGK RATMFGSVAV IRESGINSLR
     VGDVYYVGHL PWFERLWYAL ANHPILLAVL AVLAVLAAIS VILLAWVLWR LLRIISRRRL
     NPDNE