ID   BCSB_PSEFS              Reviewed;         755 AA.
AC   P58934; C3K5V5; Q8RSZ0;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Cyclic di-GMP-binding protein;
DE   AltName: Full=Cellulose synthase regulatory subunit;
GN   Name=bcsB; Synonyms=wssC; OrderedLocusNames=PFLU_0302;
OS   Pseudomonas fluorescens (strain SBW25).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=216595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12019221; DOI=10.1093/genetics/161.1.33;
RA   Spiers A.J., Kahn S.G., Bohannon J., Travisano M., Rainey P.B.;
RT   "Adaptive divergence in experimental populations of Pseudomonas
RT   fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness.";
RL   Genetics 161:33-46(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SBW25;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Binds the cellulose synthase activator, bis-(3'-5') cyclic
CC       diguanylic acid (c-di-GMP). {ECO:0000250}.
CC   -!- PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
CC   -!- SUBUNIT: Tightly associated with the cellulose synthase catalytic
CC       subunit. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AcsB/BcsB family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL71843.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AY074776; AAL71843.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AM181176; CAY46579.1; -; Genomic_DNA.
DR   RefSeq; WP_012721723.1; NC_012660.1.
DR   AlphaFoldDB; P58934; -.
DR   SMR; P58934; -.
DR   STRING; 216595.PFLU_0302; -.
DR   EnsemblBacteria; CAY46579; CAY46579; PFLU_0302.
DR   KEGG; pfs:PFLU_0302; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_003556_1_1_6; -.
DR   OMA; FQYMNPM; -.
DR   OrthoDB; 294767at2; -.
DR   UniPathway; UPA00694; -.
DR   Proteomes; UP000002332; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030244; P:cellulose biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006011; P:UDP-glucose metabolic process; IEA:InterPro.
DR   InterPro; IPR003920; Cell_synth_B.
DR   InterPro; IPR018513; Cell_synthase_bac.
DR   PANTHER; PTHR39083; PTHR39083; 1.
DR   Pfam; PF03170; BcsB; 1.
DR   PRINTS; PR01440; CELLSNTHASEB.
PE   3: Inferred from homology;
KW   c-di-GMP; Cell inner membrane; Cell membrane; Cellulose biosynthesis;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..755
FT                   /note="Cyclic di-GMP-binding protein"
FT                   /id="PRO_0000072822"
FT   TRANSMEM        724..744
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        306..318
FT                   /note="NAVVLGNPVLAGN -> TRWCWQPGIGRY (in Ref. 1; AAL71843)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   755 AA;  81763 MW;  F3784BC923225346 CRC64;
     MTSNIFARPH PRRALALMIA SLMGFNTLAQ AAEQAVATVP VQSTDTGYSL TLKQLGRRDT
     MNLQGVESSD SVNFDIRADE VVKGAQLLLK YSYSPALLAD LSQINVLVNG EVAASLPLPK
     EGAGTPQEQL VQIPAHLITE FNRLSLQFIG HYTMSCEDPL HSSLWAKISN SSELKVQVEP
     IVLKDDLAVL PLPFFDKRDA RQVSLPFVFA TAPDSAALEA AGALSSWIGG LASYRGATFP
     TTLGELPAKG NAIVLVQTAD AMDIHGVAVA KPAGPTLTLI ANPNDANGKL LIVTGRDGAE
     LKRAANAVVL GNPVLAGNSV VITKLDTLAP RRPYDAPNWL PSNRPVRLGE LIEQQKLSVS
     GYNPGAISVD MRLPPDLFNW REEGVPLKLK YRYTPQQVST NSSLLIGLND QFMKSVALPS
     VSNLGGGQTL LDQLKKDESL PREVTTLLPI SSASPKSKLQ VRFMYDYIKE GECRDIIVDN
     MRGSVDPDST LDVTGYQHYI AMPNLGVFND SGFPFTRLAD LSESAVVMPD NYGTDELTAY
     LTVLGRFGEA TGYPATAVKV VQAKDVQSVA DKDLLVLATA ANQPLLKQWQ QYLPATSDGE
     QHQFLLSDLP RYVRSWISPD PAANQHPANT GITFKGLSNS TWLAGFQSPL KSGRSVVLIA
     SNQPQGLLEA TNALIGGDDY KDSIQGSLAV VQGTQISSLV GDEQYYVGKL NYFKFMQWQL
     SQNLGWMLLI TFLGLAVVTS LIYLSLRARA KRRLA