ID   RUTD_ECOSE              Reviewed;         266 AA.
AC   B6I985;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Putative carbamate hydrolase RutD {ECO:0000255|HAMAP-Rule:MF_00832};
DE            EC=3.5.1.- {ECO:0000255|HAMAP-Rule:MF_00832};
DE   AltName: Full=Aminohydrolase {ECO:0000255|HAMAP-Rule:MF_00832};
GN   Name=rutD {ECO:0000255|HAMAP-Rule:MF_00832}; OrderedLocusNames=ECSE_1071;
OS   Escherichia coli (strain SE11).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=409438;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SE11;
RX   PubMed=18931093; DOI=10.1093/dnares/dsn026;
RA   Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H., Ooka T.,
RA   Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M.;
RT   "Complete genome sequence and comparative analysis of the wild-type
RT   commensal Escherichia coli strain SE11 isolated from a healthy adult.";
RL   DNA Res. 15:375-386(2008).
CC   -!- FUNCTION: Involved in pyrimidine catabolism. May facilitate the
CC       hydrolysis of carbamate, a reaction that can also occur spontaneously.
CC       {ECO:0000255|HAMAP-Rule:MF_00832}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=carbamate + 2 H(+) = CO2 + NH4(+); Xref=Rhea:RHEA:15649,
CC         ChEBI:CHEBI:13941, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:28938; Evidence={ECO:0000255|HAMAP-Rule:MF_00832};
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Hydrolase RutD
CC       family. {ECO:0000255|HAMAP-Rule:MF_00832}.
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DR   EMBL; AP009240; BAG76595.1; -; Genomic_DNA.
DR   RefSeq; WP_000777653.1; NC_011415.1.
DR   PDB; 3V48; X-ray; 2.10 A; A/B=1-266.
DR   PDBsum; 3V48; -.
DR   AlphaFoldDB; B6I985; -.
DR   SMR; B6I985; -.
DR   ESTHER; ecoli-rutD; RutD.
DR   EnsemblBacteria; BAG76595; BAG76595; ECSE_1071.
DR   KEGG; ecy:ECSE_1071; -.
DR   HOGENOM; CLU_020336_50_1_6; -.
DR   OMA; HAMSVTD; -.
DR   Proteomes; UP000008199; Chromosome.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR   GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   HAMAP; MF_00832; RutD; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR019913; Pyrimidine_utilisation_RutD.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR03611; RutD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase.
FT   CHAIN           1..266
FT                   /note="Putative carbamate hydrolase RutD"
FT                   /id="PRO_0000402944"
FT   STRAND          15..19
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           26..29
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           30..37
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   STRAND          40..44
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           63..76
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   STRAND          81..87
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           89..100
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   TURN            102..104
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   STRAND          105..112
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           119..148
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           151..155
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           158..170
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           175..187
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   TURN            191..193
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           194..196
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   STRAND          201..206
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   STRAND          210..212
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           215..223
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   STRAND          225..234
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           239..242
FT                   /evidence="ECO:0007829|PDB:3V48"
FT   HELIX           244..265
FT                   /evidence="ECO:0007829|PDB:3V48"
SQ   SEQUENCE   266 AA;  28898 MW;  378E13994425E9E2 CRC64;
     MKLSLSPPPY ADAPVVVLIS GLGGSGSYWL PQLAVLEQEY QVVCYDQRGT GNNPDTLAED
     YSIAQMAAEL HQALVAAGIE HYAVVGHALG ALVGMQLALD YPASVTVLIS VNGWLRINAH
     TRRCFQVRER LLYSGGAQAW VEAQPLFLYP ADWMAARAPR LEAEDALALA HFQGKNNLLR
     RLNALKRADF SHHADRIRCP VQIICASDDL LVPTACSSEL HAALPDSQKM VMPYGGHACN
     VTDPETFNAL LLNGLASLLH HREAAL